Exosomes from adipose-derived stem cells overexpressing microRNA-671–3p promote fat graft angiogenesis and adipogenic differentiation

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY Tissue & cell Pub Date : 2024-12-01 Epub Date: 2024-10-01 DOI:10.1016/j.tice.2024.102575

Xiaoyan Hao, Yuan Guo, Xueyuan Yu, Lin He, Youcheng He, Maoguo Shu

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Abstract

Exosomes from adipose-derived stem cells (ADSCs) have been demonstrated to benefit angiogenesis, wound healing, and fat grafting. Small noncoding RNAs such as microRNA (miRNA) and circular RNA play critical roles in mediating the function of ADSCs-derived exosomes. However, the underlying mechanisms have not been fully elucidated. In this study, we investigated the function and mechanism of human ADSCs-derived exosomes (hADSCs-Exo) in promoting fat graft angiogenesis and adipogenic differentiation. hADSCs-Exo were isolated and identified, and treatment with hADSCs-Exo enhanced fat graft angiogenesis and adipogenic differentiation in a mouse fat graft implantation model. We found that hADSCs-Exo overexpressed miR-671–3p and promoted human umbilical vein endothelial cell (HUVEC) proliferation, migration, and invasion. Bioinformatics analysis and luciferase reporter assay validated that TMEM127 is a direct target of miR-671–3p. Rescue experiments demonstrated that TMEM127 overexpression partially antagonized the function of hADSCs-Exo in vitro, such as suppressing HUVEC cell proliferation, migration, and invasion. Moreover, TMEM127 overexpression abrogated the function of hADSCs-Exo on fat graft angiogenesis and adipogenic differentiation. Taken together, our findings demonstrate that miR-671–3p-overexpressing exosomes from ADSC promote fat graft angiogenesis and adipogenic differentiation, which highlights the potential of targeting the ADSC-Exosomes-miR-671–3p/TMEM127 axis to improve outcome of fat graft and tissue engineering regenerative medicine.

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过表达 microRNA-671-3p 的脂肪来源干细胞外泌体可促进脂肪移植血管生成和成脂分化。

脂肪来源干细胞（ADSCs）的外泌体已被证明有利于血管生成、伤口愈合和脂肪移植。微RNA（miRNA）和环状RNA等小分子非编码RNA在介导脂肪干细胞外泌体功能方面发挥着关键作用。然而，其潜在机制尚未完全阐明。在这项研究中，我们研究了人ADSCs衍生外泌体（hADSCs-Exo）在促进脂肪移植血管生成和成脂分化方面的功能和机制。我们分离并鉴定了hADSCs-Exo，用hADSCs-Exo处理小鼠脂肪移植模型可增强脂肪移植血管生成和成脂分化。我们发现 hADSCs-Exo 过表达 miR-671-3p 并促进人脐静脉内皮细胞（HUVEC）的增殖、迁移和侵袭。生物信息学分析和荧光素酶报告实验验证了 TMEM127 是 miR-671-3p 的直接靶标。拯救实验表明，TMEM127 的过表达在体外部分拮抗了 hADSCs-Exo 的功能，如抑制 HUVEC 细胞的增殖、迁移和侵袭。此外，TMEM127 的过表达还削弱了 hADSCs-Exo 在脂肪移植血管生成和成脂分化方面的功能。综上所述，我们的研究结果表明，miR-671-3p过表达的ADSC外泌体可促进脂肪移植的血管生成和成脂分化，这凸显了靶向ADSC-外泌体-miR-671-3p/TMEM127轴改善脂肪移植和组织工程再生医学效果的潜力。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.