Comparative Study of Gleason 7 (3+4) and (4+3) Prostatic Adenocarcinomas with Prognostic Criteria and Immunohistochemical Profiles of AMACR, PSA and Ki-67.

IF 3.1 3区 医学 Q1 UROLOGY & NEPHROLOGY International Braz J Urol Pub Date : 2025-01-01 DOI:10.1590/S1677-5538.IBJU.2024.9922
Clarice F E M Osório, Waldemar S Costa, Carla B M Gallo, Luciano A Favorito, Francisco J B Sampaio
{"title":"Comparative Study of Gleason 7 (3+4) and (4+3) Prostatic Adenocarcinomas with Prognostic Criteria and Immunohistochemical Profiles of AMACR, PSA and Ki-67.","authors":"Clarice F E M Osório, Waldemar S Costa, Carla B M Gallo, Luciano A Favorito, Francisco J B Sampaio","doi":"10.1590/S1677-5538.IBJU.2024.9922","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>To compare Gleason 7 (3+4) and (4+3) prostatic adenocarcinoma (PC) with different prognostic criteria through immunohistochemical analysis with anti-PSA, anti-Ki 67 and anti-AMARC antibodies.</p><p><strong>Methods: </strong>We analyzed 221 surgical specimens from patients between 40 and 86 years-old (mean=63) with PC. The immunohistochemical study was performed with anti-PSA, anti-Ki 67 and anti-AMARC. The microscopic fields were photographed with an Olympus DP70 digital camera coupled to an Olympus BX51 microscope and archived in TIFF. Proportion and intensity criteria were used to quantify the anti-PSA antibody and for the anti-Ki 67 antibody, the quantification by similarity of this antibody in breast carcinomas. Anti-AMACR protein expression was based on four scores: negative, weak, moderate and strong. The statistical analysis was performed with the Graph Pad Prism 5 program.</p><p><strong>Results: </strong>In the Gleason score 7 (3+4) we had 91.72% in pT2 and 8.27% in the pT3 group; 8.27% recurrences, of which 90.90% in the pT2 group. In the Gleason score 7 (4+3) we had 77.27% in the pT2 group and 22.72% in the pT3 group and 10.22% of relapses, of which 66.66% in the pT2 group and 33.33% in the pT3 group. In 6.81% of cases there was an increase in the anti-Ki 67 index and in 2.27% of the cases, there was an increase in the immunoexpression of anti-p53 when comparing Gleason score 7 (3+4) with Gleason score 7 (4+3).</p><p><strong>Conclusion: </strong>Our study confirmed differences in the Gleason score 7 (3+4) and Gleason score 7 (4+3) of PC when comparing prognostic criteria. Anti-Ki 67 and anti-PSA antibody immunostaining showed a positive correlation as the Gleason score 7 increased from (3+4) to (4+3).</p>","PeriodicalId":49283,"journal":{"name":"International Braz J Urol","volume":"50 ","pages":""},"PeriodicalIF":3.1000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Braz J Urol","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1590/S1677-5538.IBJU.2024.9922","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"UROLOGY & NEPHROLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: To compare Gleason 7 (3+4) and (4+3) prostatic adenocarcinoma (PC) with different prognostic criteria through immunohistochemical analysis with anti-PSA, anti-Ki 67 and anti-AMARC antibodies.

Methods: We analyzed 221 surgical specimens from patients between 40 and 86 years-old (mean=63) with PC. The immunohistochemical study was performed with anti-PSA, anti-Ki 67 and anti-AMARC. The microscopic fields were photographed with an Olympus DP70 digital camera coupled to an Olympus BX51 microscope and archived in TIFF. Proportion and intensity criteria were used to quantify the anti-PSA antibody and for the anti-Ki 67 antibody, the quantification by similarity of this antibody in breast carcinomas. Anti-AMACR protein expression was based on four scores: negative, weak, moderate and strong. The statistical analysis was performed with the Graph Pad Prism 5 program.

Results: In the Gleason score 7 (3+4) we had 91.72% in pT2 and 8.27% in the pT3 group; 8.27% recurrences, of which 90.90% in the pT2 group. In the Gleason score 7 (4+3) we had 77.27% in the pT2 group and 22.72% in the pT3 group and 10.22% of relapses, of which 66.66% in the pT2 group and 33.33% in the pT3 group. In 6.81% of cases there was an increase in the anti-Ki 67 index and in 2.27% of the cases, there was an increase in the immunoexpression of anti-p53 when comparing Gleason score 7 (3+4) with Gleason score 7 (4+3).

Conclusion: Our study confirmed differences in the Gleason score 7 (3+4) and Gleason score 7 (4+3) of PC when comparing prognostic criteria. Anti-Ki 67 and anti-PSA antibody immunostaining showed a positive correlation as the Gleason score 7 increased from (3+4) to (4+3).

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Gleason 7 (3+4) 和 (4+3) 前列腺腺癌的预后标准与 AMACR、PSA 和 Ki-67 免疫组化图谱的比较研究。
背景:通过抗PSA、抗Ki 67和抗AMARC抗体的免疫组化分析,比较不同预后标准的Gleason 7 (3+4)和(4+3)前列腺腺癌(PC):我们分析了221例40至86岁(平均=63岁)PC患者的手术标本。用抗PSA、抗Ki 67和抗AMARC抗体进行免疫组化研究。显微镜视野用奥林巴斯 DP70 数码相机和奥林巴斯 BX51 显微镜拍摄,并以 TIFF 格式存档。采用比例和强度标准对抗-PSA 抗体进行量化,对抗-Ki 67 抗体则通过乳腺癌中该抗体的相似性进行量化。抗AMACR蛋白表达基于四种评分:阴性、弱、中等和强。统计分析使用 Graph Pad Prism 5 程序进行:在格里森评分 7(3+4)中,pT2 组占 91.72%,pT3 组占 8.27%;复发率为 8.27%,其中 pT2 组占 90.90%。在 Gleason 评分 7(4+3)的病例中,pT2 组占 77.27%,pT3 组占 22.72%;复发率为 10.22%,其中 pT2 组占 66.66%,pT3 组占 33.33%。将 Gleason 评分 7(3+4)与 Gleason 评分 7(4+3)相比较,6.81% 的病例抗 Ki 67 指数增加,2.27% 的病例抗 p53 免疫表达增加:我们的研究证实,在比较PC的预后标准时,Gleason评分7(3+4)和Gleason评分7(4+3)存在差异。抗Ki 67和抗PSA抗体免疫染色显示,随着Gleason评分7从(3+4)增加到(4+3),两者呈正相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
International Braz J Urol
International Braz J Urol UROLOGY & NEPHROLOGY-
CiteScore
4.60
自引率
21.60%
发文量
246
审稿时长
6-12 weeks
期刊介绍: Information not localized
期刊最新文献
Comparative Study of Gleason 7 (3+4) and (4+3) Prostatic Adenocarcinomas with Prognostic Criteria and Immunohistochemical Profiles of AMACR, PSA and Ki-67. Effects of Chronic Stress and Comfort Food in Testicular Morphology in Adult Wistar Rats. The Management of Children and Adolescents with Overactive Bladder Refractory to Treatment with Parasacral Transcutaneous Electrical Nerve Stimulation. 2025: The year in which the International Brazilian Journal of Urology will be able to consolidate itself as one of the most important in urology. Advancing Telesurgery Connectivity Between North and South America: the first Remote Surgery Conducted Between Orlando and São Paulo in Animal Models.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1