A Pair of Indicators for Characterizing Cerebral Microbleeds Based on Raman Spectrum and Two-Photon Imaging.

Journal of biophotonics Pub Date : 2024-10-10 DOI:10.1002/jbio.202400247

Xin Su, Jianhui Wan, Zixi Zheng, Xinyue Xing, Shengde Liu, Shuxian Yang, Liyun Zhong, Xiaoxu Lu

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Abstract

Cerebral microbleeds (CMBs) lead to cognitive decline, linked to the axonal structure composed of phospholipid bilayers. Current methods are difficult to obtain in situ changes of biochemical component concentration during CMB. In this study, by Raman spectrum and two-photon imaging, we achieve in situ changes in the information of biochemical components concentration during CMB. The overall concentration of phospholipids in the damaged tissue significantly decreases after CMB, forming a large region of low concentration, but the relative concentration of phosphatidylinositol (PI) increases, reflecting the inhibition role of the phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt) pathway. Accordingly, two-photon images of neurons show a clear decrease in the number of axons, indicating a close correlation between phospholipid hydrolysis and axon damage, as well as cognitive impairment. Therefore, the decrease in phospholipid concentration and the increase in the PI concentration might serve as a pair of indicators for characterizing CMB and its relationship with cognitive decline.

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基于拉曼光谱和双光子成像的一对脑微出血特征指标

脑微出血（CMB）会导致认知能力下降，这与由磷脂双分子层组成的轴突结构有关。目前的方法难以获得 CMB 期间生化成分浓度的原位变化。在本研究中，我们通过拉曼光谱和双光子成像技术实现了 CMB 过程中生化成分浓度信息的原位变化。CMB后，受损组织中磷脂的整体浓度明显降低，形成大片低浓度区域，但磷脂酰肌醇（PI）的相对浓度增加，反映了磷脂酰肌醇3激酶/蛋白激酶B（PI3K/Akt）通路的抑制作用。相应地，神经元的双光子图像显示轴突数量明显减少，表明磷脂水解和轴突损伤以及认知障碍之间存在密切联系。因此，磷脂浓度的降低和 PI 浓度的升高可作为描述 CMB 及其与认知功能衰退关系的一对指标。

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Journal of biophotonics

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