UV-B light (radiation) affects the metabolism of pigments and fatty acids in green algae Edaphochlorella mirabilis and Klebsormidium flaccidum in vitro

Abstract

Algae offer a rich source of bioactive compounds suitable for food products and bioenergy. Environmental challenges such as nutrient scarcity, extreme pH and temperature, high light intensity, and UV radiation usually trigger algae to produce excess of lipids, antioxidants, and other bioprotective molecules as part of their adaptations for survival. Algal cultivation provides proteins, lipids, carbohydrates, vitamins, antioxidants, and trace elements. This study focused on understanding how UV-B irradiance, as an abiotic stressor, can influence the growth and metabolite production of two green algal species, Edaphochlorella mirabilis (Chlorophyta) and Klebsormidium flaccidum (Charophyta). Using a temporary immersion system bioreactor for in-vitro algal growth, the results showed no significant difference in biomass for both algal species after the exposure to UV-B rays. However, the assessment of malondialdehyde levels revealed a significantly higher tendency towards lipid peroxidation in treated E. mirabilis (+ 90 %) compared to control. Conversely, K. flaccidum did not display significant differences, thereby highlighting a more advanced adaptive capacity against UV-B radiation. Overall, both algal species treated with UV-B showed increased pigment accumulation. K. flaccidum exhibited an average pigment increase of over 53 %, while E. mirabilis showed a lower increase, over 30 % on average. The notable rise in antioxidant compounds (lutein, β-carotene, and chlorophyll a) in UV-B exposed K. flaccidum samples also suggested a more suitable adaptive strategy to mitigate oxidative stress in Charophyta. In K. flaccidum, the increase in polyunsaturated fatty acids can be associated with increased production of antioxidant compounds. Conversely, E. mirabilis appeared to protect itself by decreasing polyunsaturated fatty acids in favor of saturated ones. In both algal species, the increase in secondary metabolites under UV stress highlighted potential as a novel food source for human consumption, deserving further investigation.