Development of root rot in Zanthoxylum bungeanum is closely linked to changes in soil microbial communities, enzyme activities, and physicochemical factors

Abstract

Due to the emergence of root rot (RR), it can cause harm to the health of Zanthoxylum bungeanum (Z. bungeanum) plants. However, the reason for this is not clear, and further research is needed. We selected healthy and RR-affected 6-year-old Z. bungeanum trees (infected for 1 year), collected their rhizosphere and bulk soils, and analyzed soil microbial communities, enzyme activities, and physicochemical properties. When the plants were diseased, soil water content (SWC), available potassium (AK), organic matter (OM) contents, β-1,4-glucosidase (β-G) activity, and fungal Shannon index significantly decreased, while bacterial Shannon index significantly increased in the rhizosphere soil. Soil total nitrogen (TN), SWC, AK, OM contents, and β-G and N-acetyl-glucosamine (NAG) activity significantly reduced, while fungal Simpson index significantly increased in the bulk soil. There were significant differences in the bacterial communities of bulk and rhizosphere soil. However, there were no obvious differences in the fungal communities. Pearson's correlation analysis showed that changes in soil microbial communities were significantly correlated with changes in the contents of TN, AK, OM, and SWC, as well as the activities of β-G and NAG. In summary, changes in soil AK, OM, SWC, and TN resulted in changes in microbial community composition, as well as changes in β-G and NAG activities, and these alterations may lead to RR in Z. bungeanum.