Chemophenetic significance and antibacterial activity of extract, various fractions and secondary metabolites from the stem bark of Ochna afzelii R. Br. (ex Oliv.)
Morel Hervé D. Kakabi , Arno R. Donfack Nanfack , Jean-De-Dieu Tamokou , James D. Simo Mpetga , Romeo Désiré T. Nago , Muhammad Shaiq Ali , Mathieu Tene , Augustin Silvère Ngouela
{"title":"Chemophenetic significance and antibacterial activity of extract, various fractions and secondary metabolites from the stem bark of Ochna afzelii R. Br. (ex Oliv.)","authors":"Morel Hervé D. Kakabi , Arno R. Donfack Nanfack , Jean-De-Dieu Tamokou , James D. Simo Mpetga , Romeo Désiré T. Nago , Muhammad Shaiq Ali , Mathieu Tene , Augustin Silvère Ngouela","doi":"10.1016/j.bse.2024.104909","DOIUrl":null,"url":null,"abstract":"<div><div>Chemical investigation of the acetone-soluble sub-fraction of the methanolic extract of the stem bark of <em>Ochna afzelii</em> R. Br. (ex Oliv.) led to the isolation of fourteen known secondary metabolites comprising two dimeric chalcones namely dihydrolophirone M <strong>(</strong><strong>1</strong><strong>)</strong> and lophirone L (<strong>2</strong>), two rearranged biflavonoids: lophirone A (<strong>3</strong>) and 4‴-methoxylophirone A (<strong>4</strong>), two biflavanone derivatives: biflavanone I (<strong>5</strong>) and biflavanone II (<strong>6</strong>), two isoflavonoids: methylirisolin (<strong>7</strong>) and biochanin A (<strong>8</strong>), one flavanol: epicatechin (<strong>9</strong>), one phenolic acid: 3,4-dihydroxybenzoic acid (<strong>10</strong>), one anthraquinone: physcion (<strong>11</strong>), two triterpenoids: lupeol (<strong>12</strong>) and oleanolic acid (<strong>13</strong>), and one steroid: sitosterol-3-<em>O</em>-<em>β</em>-<span>d</span>-glucopyranoside (<strong>14</strong>). Biochanin A (<strong>8</strong>) and 3,4-dihydroxybenzoic acid (<strong>10</strong>) are herein reported for the first time from the family Ochnaceae. The antibacterial activity was assessed <em>in vitro</em> against pathogenic strains of <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em>, <em>Staphylococcus aureus</em> and <em>Enterococcus faecalis</em>. The crude methanolic extract as well as its acetone-soluble and insoluble fractions exhibited high to significant antibacterial activity, while isolated compounds mostly displayed moderate antibacterial activity (MIC = 16–64 μg/mL).</div></div>","PeriodicalId":8799,"journal":{"name":"Biochemical Systematics and Ecology","volume":"117 ","pages":"Article 104909"},"PeriodicalIF":1.4000,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Systematics and Ecology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0305197824001273","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Chemical investigation of the acetone-soluble sub-fraction of the methanolic extract of the stem bark of Ochna afzelii R. Br. (ex Oliv.) led to the isolation of fourteen known secondary metabolites comprising two dimeric chalcones namely dihydrolophirone M (1) and lophirone L (2), two rearranged biflavonoids: lophirone A (3) and 4‴-methoxylophirone A (4), two biflavanone derivatives: biflavanone I (5) and biflavanone II (6), two isoflavonoids: methylirisolin (7) and biochanin A (8), one flavanol: epicatechin (9), one phenolic acid: 3,4-dihydroxybenzoic acid (10), one anthraquinone: physcion (11), two triterpenoids: lupeol (12) and oleanolic acid (13), and one steroid: sitosterol-3-O-β-d-glucopyranoside (14). Biochanin A (8) and 3,4-dihydroxybenzoic acid (10) are herein reported for the first time from the family Ochnaceae. The antibacterial activity was assessed in vitro against pathogenic strains of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis. The crude methanolic extract as well as its acetone-soluble and insoluble fractions exhibited high to significant antibacterial activity, while isolated compounds mostly displayed moderate antibacterial activity (MIC = 16–64 μg/mL).
期刊介绍：
Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology).
In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
