Chemophenetic significance and antibacterial activity of extract, various fractions and secondary metabolites from the stem bark of Ochna afzelii R. Br. (ex Oliv.)
Morel Hervé D. Kakabi , Arno R. Donfack Nanfack , Jean-De-Dieu Tamokou , James D. Simo Mpetga , Romeo Désiré T. Nago , Muhammad Shaiq Ali , Mathieu Tene , Augustin Silvère Ngouela
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Abstract
Chemical investigation of the acetone-soluble sub-fraction of the methanolic extract of the stem bark of Ochna afzelii R. Br. (ex Oliv.) led to the isolation of fourteen known secondary metabolites comprising two dimeric chalcones namely dihydrolophirone M (1) and lophirone L (2), two rearranged biflavonoids: lophirone A (3) and 4‴-methoxylophirone A (4), two biflavanone derivatives: biflavanone I (5) and biflavanone II (6), two isoflavonoids: methylirisolin (7) and biochanin A (8), one flavanol: epicatechin (9), one phenolic acid: 3,4-dihydroxybenzoic acid (10), one anthraquinone: physcion (11), two triterpenoids: lupeol (12) and oleanolic acid (13), and one steroid: sitosterol-3-O-β-d-glucopyranoside (14). Biochanin A (8) and 3,4-dihydroxybenzoic acid (10) are herein reported for the first time from the family Ochnaceae. The antibacterial activity was assessed in vitro against pathogenic strains of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis. The crude methanolic extract as well as its acetone-soluble and insoluble fractions exhibited high to significant antibacterial activity, while isolated compounds mostly displayed moderate antibacterial activity (MIC = 16–64 μg/mL).
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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