Molecular characterization of Fasciola hepatica obtained from cattle and horse in Central Chile

IF 1.4 Q3 PARASITOLOGY Veterinary parasitology, regional studies and reports Pub Date : 2024-09-27 DOI:10.1016/j.vprsr.2024.101130

Gonzalo Cabrera , Carolina Cabezas , Daniela Estay-Olea , Caroll Stoore , María Soledad Baquedano , Rodolfo Paredes , Christian Hidalgo

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Abstract

Liver fluke infection, caused by the trematode Fasciola hepatica, is a parasitic zoonotic disease affecting various mammals, including humans, and has significant implications for public, animal, and ecosystem health. This study provides the first genetic characterization of F. hepatica in Chile, focusing on the complete mitochondrial gene cox1. Samples were collected from two different host species: cattle and horses. Our findings revealed that 70 % of detected haplotypes were found in either cattle or horses, which coincides with their geographical origin. Interestingly, the use of full-length sequences resulted in the identification of 80 % unique sequences, whereas this reduced to 45 % when analyzing the traditionally used short sequences. This underestimation of genetic diversity suggests that broader sequencing efforts might be essential for a more accurate understanding of F. hepatica genetic landscape. This research underscores the importance of understanding the genetic variability in parasites to improve strategies for disease control and treatment.

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智利中部从牛和马身上获得的肝包虫病的分子特征

由肝吸虫引起的肝吸虫感染是一种影响包括人类在内的各种哺乳动物的寄生性人畜共患疾病，对公众、动物和生态系统健康有重大影响。本研究首次对智利的肝吸虫进行了基因鉴定，重点研究了完整的线粒体基因 cox1。样本采集自两种不同的宿主物种：牛和马。我们的研究结果表明，所检测到的单倍型中有 70% 是在牛或马身上发现的，这与它们的地理来源相吻合。有趣的是，使用全长序列可鉴定出 80% 的独特序列，而分析传统使用的短序列时，这一比例降至 45%。这种对遗传多样性的低估表明，更广泛的测序工作对于更准确地了解肝吸虫遗传景观可能是至关重要的。这项研究强调了了解寄生虫基因变异性对改进疾病控制和治疗策略的重要性。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).