Quantification measurement of hemoglobin with large dynamic range and low detection limit via an optical fiber optofluidic laser with enzyme-catalyzed reaction

IF 4.1 3区工程技术 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC Sensors and Actuators A-physical Pub Date : 2024-10-01 DOI:10.1016/j.sna.2024.115936

Hongrui Zhang , Ya-nan Zhang , Mingyue Wang , Yong Zhao , Bo Han

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Abstract

Quantitative measurement of hemoglobin (Hb) levels is an essential part of routine medical examinations, disease diagnosis and health status monitoring. In this study, we introduced optical fiber optofluidic laser (FOFL) technology combined with catalytic oxidation reaction to design a laser sensor for sensitive Hb detection. For the H₂O₂-rhodamine B (RhB) oxidation system, radially emitting FOFL was achieved with a thin-walled hollow optical fiber (HOF) as an optical microcavity and amplified the concentration change of RhB during oxidation by H₂O₂. Hb was employed as peroxide-mimicking enzyme to catalyze the oxidation system, which sped up the reaction, resulting in an earlier laser extinction time. With the laser extinction time as sensing signal, the Hb sensor achieved a dynamic range of five orders of magnitude and a limit of detection (LOD) of 46.0 pM at an assay time of 40 min. The developed method provides ideas for the exploitation of FOFL biosensing based on catalytic oxidation reactions.

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通过光纤光流体激光器与酶催化反应定量测量血红蛋白，动态范围大，检测限低

血红蛋白（Hb）水平的定量测量是常规体检、疾病诊断和健康状况监测的重要组成部分。在这项研究中，我们引入了光纤光流体激光器（FOFL）技术，并结合催化氧化反应设计了一种用于灵敏检测 Hb 的激光传感器。在 H2O2-罗丹明 B（RhB）氧化体系中，利用薄壁中空光纤（HOF）作为光学微腔，实现了径向发射的 FOFL，并放大了 RhB 在 H2O2 氧化过程中的浓度变化。利用 Hb 作为过氧化物模拟酶催化氧化系统，加快了反应速度，从而提前了激光消光时间。以激光熄灭时间作为传感信号，Hb 传感器的动态范围达到五个数量级，检测时间为 40 分钟，检测限（LOD）为 46.0 pM。所开发的方法为利用基于催化氧化反应的 FOFL 生物传感提供了思路。

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来源期刊

Sensors and Actuators A-physical 工程技术-工程：电子与电气

CiteScore

8.10

自引率

6.50%

发文量

630

审稿时长

49 days

期刊介绍： Sensors and Actuators A: Physical brings together multidisciplinary interests in one journal entirely devoted to disseminating information on all aspects of research and development of solid-state devices for transducing physical signals. Sensors and Actuators A: Physical regularly publishes original papers, letters to the Editors and from time to time invited review articles within the following device areas: • Fundamentals and Physics, such as: classification of effects, physical effects, measurement theory, modelling of sensors, measurement standards, measurement errors, units and constants, time and frequency measurement. Modeling papers should bring new modeling techniques to the field and be supported by experimental results. • Materials and their Processing, such as: piezoelectric materials, polymers, metal oxides, III-V and II-VI semiconductors, thick and thin films, optical glass fibres, amorphous, polycrystalline and monocrystalline silicon. • Optoelectronic sensors, such as: photovoltaic diodes, photoconductors, photodiodes, phototransistors, positron-sensitive photodetectors, optoisolators, photodiode arrays, charge-coupled devices, light-emitting diodes, injection lasers and liquid-crystal displays. • Mechanical sensors, such as: metallic, thin-film and semiconductor strain gauges, diffused silicon pressure sensors, silicon accelerometers, solid-state displacement transducers, piezo junction devices, piezoelectric field-effect transducers (PiFETs), tunnel-diode strain sensors, surface acoustic wave devices, silicon micromechanical switches, solid-state flow meters and electronic flow controllers. Etc...