ViCE: An automated and quantitative program to assess intestinal tissue morphology

Q2 Medicine Journal of Pathology Informatics Pub Date : 2024-09-13 DOI:10.1016/j.jpi.2024.100397

Jeffrey La , Krishnan Raghunathan , Jocelyn A. Silvester , Jay R. Thiagarajah

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Abstract

Background and objective

The tissue morphology of the intestinal surface is architecturally complex with finger-like projections called villi, and glandular structures called crypts. The ratio of villus height-to-crypt depth ratio (Vh:Cd) is used to quantitatively assess disease severity and response to therapy for intestinal enteropathies, such as celiac disease and is currently quantified manually. Given the time required, manual Vh:Cd measurements have largely been limited to clinical trials and are not used widely in clinical practice. We developed ViCE (Villus Crypt Evaluator), a user-friendly software that automatically quantifies histological parameters in standard hematoxylin and eosin-stained intestinal biopsies.

Methods

ViCE is based on mathematical morphology operations and is scale and staining agnostic. It evaluates tissue orientation, identifies geometrical structure, and outputs key tissue measurements.

Results

The output measurements of Vh:Cd are concordant with manual quantifications across multiple datasets.

Conclusions

The underlying mathematical morphological approach for ViCE is robust, and reproducible and easily adaptable for measurement of morphological features in other tissues.

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ViCE：自动定量评估肠道组织形态的程序

背景和目的肠表面的组织形态结构复杂，有称为绒毛的指状突起和称为隐窝的腺体结构。绒毛高度与隐窝深度之比（Vh:Cd）用于定量评估疾病的严重程度以及对乳糜泻等肠道疾病的治疗反应，目前采用人工定量的方法。由于需要花费大量时间，手动 Vh:Cd 测量在很大程度上仅限于临床试验，并未广泛应用于临床实践。我们开发了 ViCE（绒毛隐窝评价器），这是一款用户友好型软件，可自动量化标准苏木精和伊红染色肠活检组织学参数。结果在多个数据集中，Vh:Cd 的输出测量结果与人工量化结果一致。结论ViCE 的基本数学形态学方法是稳健的、可重复的，并且很容易适应于其他组织形态特征的测量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Pathology Informatics Medicine-Pathology and Forensic Medicine

CiteScore

3.70

自引率

0.00%

发文量

审稿时长

18 weeks

期刊介绍： The Journal of Pathology Informatics (JPI) is an open access peer-reviewed journal dedicated to the advancement of pathology informatics. This is the official journal of the Association for Pathology Informatics (API). The journal aims to publish broadly about pathology informatics and freely disseminate all articles worldwide. This journal is of interest to pathologists, informaticians, academics, researchers, health IT specialists, information officers, IT staff, vendors, and anyone with an interest in informatics. We encourage submissions from anyone with an interest in the field of pathology informatics. We publish all types of papers related to pathology informatics including original research articles, technical notes, reviews, viewpoints, commentaries, editorials, symposia, meeting abstracts, book reviews, and correspondence to the editors. All submissions are subject to rigorous peer review by the well-regarded editorial board and by expert referees in appropriate specialties.