Impact of alkaline solution, carbohydrase and biomass:solvent on extraction efficiency, and protein and amino acid profiles of Palmaria palmata protein extracts
Anthony Temitope Idowu , Miryam Amigo-Benavent , Richard.J. FitzGerald
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引用次数: 0
Abstract
The impact of using different alkaline solutions, carbohydrases and changing the biomass to solvent volume ratio (biomass:solvent) on protein content, protein and biomass yield recovery in protein enriched extracts from Palmaria palmata (red seaweed) was studied. Protein extraction with alkaline solutions, i.e., NaOH, KOH, Ca(OH)2 and Na2CO3 (at 0.12 M) along with (and without) carbohydrases B, BD and L (containing xylanase, a mixture of xylanase and cellulase, and a combination of cellulase, xylanase and polygalacturonase activities, respectively) was assessed. Principal component analysis was used to assess correlations (if any) between the different alkaline solvents, carbohydrases and changing biomass:solvent on extraction efficiency. The highest protein content (56.64 ± 0.43 % (w/w)) was obtained using KOH assisted extraction with carbohydrase BD. The highest protein and biomass yield recoveries (70.65 ± 1.38 and 21.99 ± 0.43 % (w/w), respectively) were obtained on extraction using NaOH assisted with carbohydrases BD. Electrophoretic, amino acid profile and amino acid score analyses displayed differences depending on the alkaline solvent used for protein extraction. Appropriate selection of the alkaline solvent, carbohydrase and biomass:volume combination during protein extraction can contribute to the production of compositionally enhanced high-quality protein concentrates from P. palmata.
期刊介绍：
Official Journal of the European Federation of Chemical Engineering:
Part C
FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering.
Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing.
The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those:
• Primarily concerned with food formulation
• That use experimental design techniques to obtain response surfaces but gain little insight from them
• That are empirical and ignore established mechanistic models, e.g., empirical drying curves
• That are primarily concerned about sensory evaluation and colour
• Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material,
• Containing only chemical analyses of biological materials.
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