Metabolomics analysis of Cucumis melo var. flexuosus organs in correlation to its anti-inflammatory activity aided by chemometrics

IF 3.1 3区医学 Q2 CHEMISTRY, ANALYTICAL Journal of pharmaceutical and biomedical analysis Pub Date : 2024-10-09 DOI:10.1016/j.jpba.2024.116512

Hesham M. El-Sayed , Dalia M. Rasheed , Engy A. Mahrous , Basma M. Eltanany , Zeinab M. Goda , Laura Pont , Fernando Benavente , Essam Abdel-Sattar

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Abstract

Snake melon (Cucumis melo var. flexuosus, CM) is a gourd with health-promoting nutritional traits and unexplored phytochemicals. This study aims to comprehensively investigate the phytoconstituents in the fruits, leaves, roots, seeds, and stems of CM, using liquid chromatography-quadrupole time-of-flight tandem mass spectrometry. Consequently, 118 metabolites were identified, encompassing phenolic compounds, flavonoids, megastigmanes, lignans, cucurbitacins, and fatty acids. Multivariate data analysis revealed differences in the metabolite composition of CM organs and correlated these variations with the potential in-vitro anti-inflammatory properties assessed against RAW 264.7 macrophages through the down-regulation of cyclo-oxygenase-Ⅱ, nuclear factor-kappa B, and tumor necrosis factor-α. The results indicated that leaf and seed extracts showed the highest anti-inflammatory activity due to their enrichment in several flavonoids, phenolic glycosides, and a megastigmane. These findings emphasize the health benefits of CM organs as potential functional foods and functional food by-products, serving as a natural source for developing new anti-inflammatory agents.

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利用化学计量学分析甜瓜变种（Cucumis melo var. flexuosus）器官的代谢组学与其抗炎活性的相关性

蛇瓜（Cucumis melo var. flexuosus，CM）是一种具有促进健康的营养特性和尚未开发的植物化学物质的瓜类。本研究旨在利用液相色谱-四极杆飞行时间串联质谱法全面研究蛇瓜果实、叶、根、种子和茎中的植物成分。结果共鉴定出 118 种代谢物，包括酚类化合物、黄酮类化合物、巨豆素、木脂素、葫芦素和脂肪酸。多变量数据分析揭示了中药各器官代谢物组成的差异，并将这些差异与通过下调环氧化酶-Ⅱ、核因子-kappa B 和肿瘤坏死因子-α对 RAW 264.7 巨噬细胞进行的体外抗炎特性评估相关联。结果表明，叶片和种子提取物的抗炎活性最高，这是因为它们富含几种黄酮类化合物、酚苷类化合物和一种巨甙。这些发现强调了中药器官作为潜在功能食品和功能食品副产品的健康益处，是开发新型抗炎剂的天然来源。

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来源期刊

Journal of pharmaceutical and biomedical analysis 医学-分析化学

CiteScore

6.70

自引率

5.90%

发文量

588

审稿时长

37 days

期刊介绍： This journal is an international medium directed towards the needs of academic, clinical, government and industrial analysis by publishing original research reports and critical reviews on pharmaceutical and biomedical analysis. It covers the interdisciplinary aspects of analysis in the pharmaceutical, biomedical and clinical sciences, including developments in analytical methodology, instrumentation, computation and interpretation. Submissions on novel applications focusing on drug purity and stability studies, pharmacokinetics, therapeutic monitoring, metabolic profiling; drug-related aspects of analytical biochemistry and forensic toxicology; quality assurance in the pharmaceutical industry are also welcome. Studies from areas of well established and poorly selective methods, such as UV-VIS spectrophotometry (including derivative and multi-wavelength measurements), basic electroanalytical (potentiometric, polarographic and voltammetric) methods, fluorimetry, flow-injection analysis, etc. are accepted for publication in exceptional cases only, if a unique and substantial advantage over presently known systems is demonstrated. The same applies to the assay of simple drug formulations by any kind of methods and the determination of drugs in biological samples based merely on spiked samples. Drug purity/stability studies should contain information on the structure elucidation of the impurities/degradants.