Supercritical CO2 impregnation of pink pepper essential oil in gelatin-siloxane cryogel for biomedical applications

IF 3.4 3区工程技术 Q2 CHEMISTRY, PHYSICAL Journal of Supercritical Fluids Pub Date : 2024-09-30 DOI:10.1016/j.supflu.2024.106419

Ulisses Silva do Nascimento , Thayná dos Santos Borges , Carolina Kafka Neves , Ariane Moracci Yoshitake , Elisete Vieira do Nascimento , Christiane Bertachini Lombello , Bruno Guzzo da Silva , Lígia Passos Maia-Obi

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Abstract

The supercritical CO₂ impregnation of pink pepper essential oil (EO) can bring its bioactive properties to a biomaterial in a green approach, making it multifunctional. Aiming to obtain a gelatin-siloxane cryogel loaded with pink pepper EO for biomedical applications and to understand the impact of the impregnation conditions, gelatin was crosslinked with (3-glycidoxypropyl)trimethoxysilane and lyophilized, and the impregnation was studied using a design of experiments with conditions from 10 to 30 MPa and 35–60 °C. The cryogel synthesis was confirmed by FTIR, %gel and TGA. Its biocompatibility was demonstrated through SEM, water uptake and cytotoxicity studies. It was shown that, due to the influence on the solubility of the EO in scCO₂, varying pressure and temperature influences the impregnation, which showed {20 MPa; 35 °C} as its best condition. Therefore, a cryogel suitable for implantation with potential to reduce side effects related with infection and inflammation was obtained.

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超临界二氧化碳浸渍粉红胡椒精油在明胶-硅氧烷低温凝胶中的生物医学应用

对粉红胡椒精油（EO）进行超临界二氧化碳浸渍可将其生物活性特性以绿色方法注入生物材料，使其具有多功能性。为了获得负载粉红胡椒精油的明胶-硅氧烷低温凝胶用于生物医学应用，并了解浸渍条件的影响，研究人员用（3-缩水甘油氧丙基）三甲氧基硅烷交联明胶并将其冻干，然后在 10 至 30 兆帕和 35 至 60 °C的条件下采用实验设计法研究了浸渍过程。傅立叶变换红外光谱（FTIR）、凝胶百分比（%gel）和热重分析（TGA）证实了冷冻凝胶的合成。通过扫描电子显微镜、吸水率和细胞毒性研究证明了其生物相容性。研究表明，由于环氧乙烷在 scCO2 中的溶解度会受到影响，因此不同的压力和温度会影响浸渍效果。因此，我们获得了一种适合植入的低温凝胶，它有可能减少与感染和炎症有关的副作用。

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来源期刊

Journal of Supercritical Fluids 工程技术-工程：化工

CiteScore

7.60

自引率

10.30%

发文量

236

审稿时长

56 days

期刊介绍： The Journal of Supercritical Fluids is an international journal devoted to the fundamental and applied aspects of supercritical fluids and processes. Its aim is to provide a focused platform for academic and industrial researchers to report their findings and to have ready access to the advances in this rapidly growing field. Its coverage is multidisciplinary and includes both basic and applied topics. Thermodynamics and phase equilibria, reaction kinetics and rate processes, thermal and transport properties, and all topics related to processing such as separations (extraction, fractionation, purification, chromatography) nucleation and impregnation are within the scope. Accounts of specific engineering applications such as those encountered in food, fuel, natural products, minerals, pharmaceuticals and polymer industries are included. Topics related to high pressure equipment design, analytical techniques, sensors, and process control methodologies are also within the scope of the journal.