A readily accessible quaternized cellulose filter paper with high permeability for IgG separation

IF 6.5 2区 材料科学 Q1 MATERIALS SCIENCE, COMPOSITES Composites Communications Pub Date : 2024-10-05 DOI:10.1016/j.coco.2024.102112
Tiange Chen , Pan Cheng , Mingyue Li , Yuxi Wang , Peiling Tang , Yinuo Zhang , Qihao Guo , Qin Cheng , Tao Mei , Ke Liu , Dong Wang
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Abstract

Anion-exchange chromatography (AEC) is recognized as a highly effective approach for the purification of immunoglobulin G (IgG). This study introduces an innovative strategy that employs waste cellulose filter paper in the production of AEC media. A quaternized cellulose fiber membrane (CFM-QCS) was successfully fabricated that cellulose fibers were as a structural framework, glutaraldehyde (GA) as a crosslinking agent, and quaternary chitosan (QCS) as a modifying agent. Morphological and chemical characterization revealed that GA and QCS were uniformly crosslinked on the surface of the cellulose fibers, resulting in excellent mechanical properties in both dry and wet states. Benefiting from its 3D network scaffold structure, CFM-QCS demonstrated a high adsorption capacity for bovine serum albumin (BSA), with static and dynamic adsorption capacities of 605.15 mg/g and 88.63 mg/ml, respectively. After treated with extreme conditions and 10 cyclic adsorption and elution, the adsorption capacity of CFM-QCS remains almost unchanged, highlighting its excellent stability. Additionally, a CFM-QCS packed chromatography column exhibited high flux of 10.38 L/h at 0.1 MPa, which can efficiently separate IgG from a mixed solution in the presence of BSA and IgG by gravity-driven. This work presents a straightforward approach for preparing high-performance ion-exchange chromatography membranes for IgG separation.

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一种易于获得的高渗透性季铵化纤维素滤纸,可用于分离 IgG
阴离子交换色谱法(AEC)是公认的纯化免疫球蛋白 G(IgG)的高效方法。本研究介绍了一种利用废纤维素滤纸生产 AEC 培养基的创新策略。以纤维素纤维为结构框架,戊二醛(GA)为交联剂,季甲壳素(QCS)为改性剂,成功制备了季铵化纤维素纤维膜(CFM-QCS)。形态和化学特性分析表明,GA 和 QCS 在纤维素纤维表面均匀交联,因此在干态和湿态下都具有优异的机械性能。得益于其三维网络支架结构,CFM-QCS 对牛血清白蛋白(BSA)具有很高的吸附能力,静态和动态吸附能力分别为 605.15 mg/g 和 88.63 mg/ml。在经过极端条件处理和 10 次循环吸附和洗脱后,CFM-QCS 的吸附容量几乎保持不变,这突出表明了其出色的稳定性。此外,CFM-QCS 填料色谱柱在 0.1 MPa 压力下的通量高达 10.38 L/h,可在重力驱动下从含有 BSA 和 IgG 的混合溶液中有效分离出 IgG。本研究提出了一种制备用于分离 IgG 的高性能离子交换色谱膜的直接方法。
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来源期刊
Composites Communications
Composites Communications Materials Science-Ceramics and Composites
CiteScore
12.10
自引率
10.00%
发文量
340
审稿时长
36 days
期刊介绍: Composites Communications (Compos. Commun.) is a peer-reviewed journal publishing short communications and letters on the latest advances in composites science and technology. With a rapid review and publication process, its goal is to disseminate new knowledge promptly within the composites community. The journal welcomes manuscripts presenting creative concepts and new findings in design, state-of-the-art approaches in processing, synthesis, characterization, and mechanics modeling. In addition to traditional fiber-/particulate-reinforced engineering composites, it encourages submissions on composites with exceptional physical, mechanical, and fracture properties, as well as those with unique functions and significant application potential. This includes biomimetic and bio-inspired composites for biomedical applications, functional nano-composites for thermal management and energy applications, and composites designed for extreme service environments.
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