Physiological and molecular responses of juvenile silver crucian carp (Carassius gibelio) to long-term high alkaline stress: Growth performance, histopathology, and transcriptomic analysis

Abstract

Salty-alkaline waters are widely distributed globally, and how to effectively develop saline-alkaline water to improve the utilization rate of water resources has become a global challenge. This study examined the survival performance of juvenile silver crucian carp (Carassius gibelio) under alkaline gradient acclimation conditions and explored the regulatory mechanisms for their adaptation to high alkalinity. Compared to directly immersing in high concentrations, alkaline gradient acclimation significantly improved the survival rate of juvenile silver crucian carp. Long-term high alkaline stress caused significant reduction in the growth performance of juvenile silver crucian carp. After alkaline stress, the enzyme activities of Na⁺-K⁺-ATP (NKA) and Ca²⁺-Mg²⁺-ATP (CMA) in the gills were significantly decreased. Meanwhile, histopathology of the gills showed structural changes, indicating that the physiological functions of juvenile silver crucian carp were impaired under alkaline conditions. Transcriptomic analysis found that the expression of genes related to metabolism and immune response pathways in the gill and kidney underwent significant changes. Genes related to carbohydrate metabolism were upregulated while those related to protein metabolism were downregulated, indicating that alkaline stress caused the organism to utilize carbohydrates rather than proteins as much as possible to meet enhanced metabolism. The HIF pathway that maintains the body's survival was significantly up-regulated, at the expense of high energy consumption. Dysregulation of genes related to the formation of neutrophil extracellular traps (NETosis) pathway indicated that the immune function of the fish was suppressed. Overall, this study revealed the physiological and molecular responses of juvenile silver crucian carp to alkaline stress, deepening our understanding of their adaptation strategies and the potential impact of alkaline environments on aquatic life.