Differences in succession of bacterial communities during co-cultivation of corn straw with different soils

IF 3.7 2区 农林科学 Q1 ECOLOGY European Journal of Soil Biology Pub Date : 2024-10-13 DOI:10.1016/j.ejsobi.2024.103683
Shuang Liu, Qingxin Meng, Yujia Li, Zhigang Wang, Weihui Xu, Yingning Sun, Zhidan Yu, Yunlong Hu
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Abstract

Managing carbon inputs from straw can pave the way towards carbon neutrality and climate change mitigation. Straw decomposition by cooperative microbial actions is an important process of carbon cycling in nature, and in this process, microbial communities are constantly in succession. Soil is rich in microorganisms and can be a source of microbial for straw degradation. In this study, corn straw was mixed with different soil types and incubated in conical flasks for 70 days. Bacterial diversity and community structure were determined using 16S rRNA sequencing. Then, the effects of physicochemical parameters and enzyme activities on the composition of bacterial communities at different stages were evaluated. The results showed that bacterial diversity decreased during co-cultivation. The differences in bacterial communities between all treatments were greater in the later stages, with Pseudomonadota, Actinomycetota, and Bacillota as the major phyla. Among them, the biomarkers at different times for different treatments included Sphingomonas, Mycobacterium, Oceanobacillus, Streptomyces, Pseudomonas, Flavobacterium, and Saccharomonospora. All of them showed cellulose degradation capacity; thus, the organic matter gradually decreased during the co-cultivation. Canonical correspondence analysis (CCA) showed that pH, organic matter (OM), electrical conductivity (EC), cellulase, β-glucosidase, and filter paper (FPase) activities had a significant effect on bacterial communities at different stages. Our findings suggested that soil microbial communities can be an effective source of cellulose-degrading microorganisms, and corn straw co-cultivation with different soil types increased the abundance of cellulose-degrading bacteria, which provides the theoretical basis for efficient cellulose-degrading agent screening.
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玉米秸秆与不同土壤共耕期间细菌群落演替的差异
管理来自秸秆的碳输入可以为实现碳中和和减缓气候变化铺平道路。微生物合作分解秸秆是自然界碳循环的一个重要过程,在此过程中,微生物群落不断更替。土壤中含有丰富的微生物,可以作为秸秆降解的微生物源。本研究将玉米秸秆与不同类型的土壤混合,并在锥形瓶中培养 70 天。使用 16S rRNA 测序测定了细菌多样性和群落结构。然后,评估了不同阶段理化参数和酶活性对细菌群落组成的影响。结果表明,细菌多样性在共培养过程中有所下降。所有处理之间的细菌群落差异在后期阶段都较大,主要以假单胞菌、放线菌和芽孢杆菌为主。其中,不同处理不同时间的生物标志物包括鞘氨单胞菌、分枝杆菌、海洋杆菌、链霉菌、假单胞菌、黄杆菌和酵母菌。它们都具有降解纤维素的能力,因此在共培养过程中有机物质逐渐减少。典型对应分析(CCA)表明,pH 值、有机质(OM)、电导率(EC)、纤维素酶、β-葡萄糖苷酶和滤纸酶(FPase)活性对不同阶段的细菌群落有显著影响。我们的研究结果表明,土壤微生物群落可作为纤维素降解微生物的有效来源,玉米秸秆与不同类型土壤共培养可提高纤维素降解菌的丰度,这为高效纤维素降解剂的筛选提供了理论依据。
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来源期刊
European Journal of Soil Biology
European Journal of Soil Biology 环境科学-生态学
CiteScore
6.90
自引率
0.00%
发文量
51
审稿时长
27 days
期刊介绍: The European Journal of Soil Biology covers all aspects of soil biology which deal with microbial and faunal ecology and activity in soils, as well as natural ecosystems or biomes connected to ecological interests: biodiversity, biological conservation, adaptation, impact of global changes on soil biodiversity and ecosystem functioning and effects and fate of pollutants as influenced by soil organisms. Different levels in ecosystem structure are taken into account: individuals, populations, communities and ecosystems themselves. At each level, different disciplinary approaches are welcomed: molecular biology, genetics, ecophysiology, ecology, biogeography and landscape ecology.
期刊最新文献
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