European Journal of Soil Biology最新文献

{"title":"Regulation of nutrient homeostasis by soil microbial functional differentiation and metabolic limitations under interactive forest type and Stropharia rugosoannulata cultivation","authors":"Suli Ke ,&nbsp;Tingyan Liu ,&nbsp;Longfei Hao ,&nbsp;Tuya Sharen ,&nbsp;Dongmei Ye ,&nbsp;Yuan Wang ,&nbsp;Yuanlin Wang ,&nbsp;Xiaoqiang Wang","doi":"10.1016/j.ejsobi.2026.103804","DOIUrl":"10.1016/j.ejsobi.2026.103804","url":null,"abstract":"<div><div>The forest-mushroom symbiosis model is a sustainable agroforestry practice; however, the microbial mechanisms driving nutrient cycling under different cultivation patterns remain unclear. This study systematically compared the effects of single (2022) versus continuous (2022 + 2024) cultivation of <em>Stropharia rugosoannulata</em> on soil microbial communities, metabolic limitations, and nutrient dynamics in three contrasting forest types: <em>Larix gmelinii</em> plantation, <em>Populus davidiana</em> plantation, and a natural secondary forest. Key findings revealed that (1) continuous cultivation universally enhanced microbial biomass carbon (+29.2 % to +83.2 %) but exacerbated carbon limitation, while shifting the primary nutrient limitation from nitrogen in plantations to phosphorus in the secondary forest. (2) Cultivation reshaped microbial communities, enriching copiotrophic bacteria (Proteobacteria and Firmicutes) and differentially stimulating fungal decomposers (Ascomycota in single-cultivation <em>P. davidiana</em> and Basidiomycota in continuous-cultivation secondary forest) (3) These microbial shifts drove divergent nutrient pathways: continuous cultivation boosted available phosphorus and total nutrients in <em>L. gmelinii</em> plantations, whereas single cultivation optimized available nitrogen in <em>P. davidiana</em> plantations. (4) Integrated path analysis revealed that forest type governs total nutrient cycling via metabolic limitations on microbial structure, whereas cultivation mode regulates available nutrients through metabolic constraints, with bacterial abundance as the core positive driver. Unlike previous studies focusing on single environments or substrates, the comprehensive pathway model proposed in this research reveals that continuously cultivated <em>S. rugosoannulata</em> regulates soil nutrient cycling through forest-type-dependent microbial pathways. This finding elucidates the underlying mechanisms behind its differential effects in plantation and secondary forest soils, thereby providing a theoretical foundation for the precision management of forest fungal systems.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"128 ","pages":"Article 103804"},"PeriodicalIF":3.3,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of understory degradation induced by sika deer on soil microarthropods in beech forests: comparison between two different climatic conditions","authors":"Erika Kawakami ,&nbsp;Takuo Hishi ,&nbsp;Ayumi Katayama","doi":"10.1016/j.ejsobi.2026.103803","DOIUrl":"10.1016/j.ejsobi.2026.103803","url":null,"abstract":"<div><div>Understory vegetation dominated by dwarf bamboo (<em>Sasa</em> spp.) has been decreasing in Japan because of deer overgrazing. Declining understory vegetation may reduce soil microarthropods abundance. However, the effects of understory degradation on soil microarthropods under various climatic conditions remain poorly understood. We compared soil microarthropods, soil properties, and erosion rates (cm year⁻¹) between rich understory plots (<em>Sasa</em>) and no understory plots (No<em>Sasa</em>) in six beech forests in Kyushu and San-in. The Kyushu region experiences more rainfall and less snow depth than the San-in region. In Kyushu, the abundance of soil microarthropods was 40 % significantly lower in the No<em>Sasa</em> plot than in the <em>Sasa</em> plot, whereas there were no significant differences in San-in. In both regions, soil bulk density was significantly higher in the No<em>Sasa</em> plot, but the effects of understory degradation were larger in Kyushu. These results suggest that understory degradation leads to soil runoff and reduces habitable pore spaces for soil microarthropods. Additionally, structural equation modelling analysis indicated that soil erosion following understory degradation was influenced by climatic conditions, and the increase in bulk density induced by soil erosion reduced the abundance of soil microarthropods. This finding suggests that the effects of understory degradation on soil microarthropods via changes in bulk density vary depending on climatic conditions and are stronger in erosion-prone areas. Our results imply that prevention measures for understory vegetation are urgently needed to maintain soil biodiversity, particularly in regions with climatic conditions that are highly susceptible to soil erosion.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"128 ","pages":"Article 103803"},"PeriodicalIF":3.3,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145938884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochar amendment slowed down the decomposition of wheat residue by suppressing the population of wheat residue-decomposers in bacterial community","authors":"Benjuan Liu ,&nbsp;Zijie Liu ,&nbsp;Wanqin Yang ,&nbsp;Dan Wang ,&nbsp;Qi Liu ,&nbsp;Yifan Li ,&nbsp;Jianfeng Hou ,&nbsp;Zubin Xie","doi":"10.1016/j.ejsobi.2025.103802","DOIUrl":"10.1016/j.ejsobi.2025.103802","url":null,"abstract":"<div><div>Straw returning is vital for supplying available carbon to maintain microbial functions. The effects of irreversible biochar accumulation, resulting from long-term continuous biochar application or one-time high-dose application, on straw decomposition are of great importance to agricultural practices, yet they remain poorly understood. Therefore, a 30-day laboratory incubation experiment was conducted to investigate the straw decomposition rate and the functional bacteria involved in the decomposition process under high-dose biochar amendment (9 %, w/w), employing ¹³C-labeled wheat residue and ¹³C-DNA stable isotope probing technique coupled with high-throughput sequencing, following three treatments: soil only, soil added with wheat residue, and soil added with both wheat residue and biochar. Results showed that biochar markedly reduced the decomposition rate of wheat residue (<em>p</em> &lt; 0.05), which was attributed to its suppression of the population size of wheat-residue-decomposing bacteria (<em>p</em> &lt; 0.05). However, both the Shannon-Wiener diversity index and the Pielou evenness index of the wheat residue-decomposer community were significantly elevated, and neither showed a significantly positive relationship with the decomposition rate. The families <em>Oxalobacteraceae</em>, <em>Burkholderiaceae</em>, and <em>Xanthomonadaceae</em> were the dominant wheat-residue decomposers, and their population sizes were markedly suppressed by biochar, particularly during the early stages of decomposition. <em>Paenibacillaceae 1</em>, mainly comprising the genera <em>Paenibacillus</em> and <em>Cohnella</em>, was a unique plant residue-decomposer and stimulated in the presence of biochar, suggesting its preference for the high pH conditions created by biochar. This study concluded that large amounts of accumulated biochar will aggravate the difficulty in straw decomposition, potentially harming the straw returning measure.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"128 ","pages":"Article 103802"},"PeriodicalIF":3.3,"publicationDate":"2025-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Responses of the earthworms Lumbricus terrestris and Aporrectodea caliginosa to wheat straw provision across a range of residue sizes","authors":"Peter Bentley,&nbsp;Kevin R. Butt","doi":"10.1016/j.ejsobi.2025.103799","DOIUrl":"10.1016/j.ejsobi.2025.103799","url":null,"abstract":"<div><div>Earthworm mediated incorporation of soil surface applied crop residues could provide benefits to belowground ecosystem services, such as an increased rate of soil formation and carbon sequestration. In addition, increased soil organic matter within the upper soil profile can increase food availability for other soil fauna and microorganisms, with potential benefits for soil structure and health. Previous research has assessed the potential mass of surface applied organic matter that can be assimilated by earthworms; however, particle size of material may limit the rate of bioturbation and influence earthworm function and behaviour. The aims of the present study were to investigate the preference and utilisation of wheat (<em>Triticum aestivum</em>) straw residues at different particle sizes by two common, temperate earthworm species, <em>Lumbricus terrestris</em> and <em>Aporrectodea caliginosa.</em> These were addressed within controlled laboratory experiments, where two different scales were tested: Expt 1; with 3 modal straw lengths, as determined from the field post-harvest (40, 20 and 1 cm); and Expt 2; micro particle sizes (1 cm and 1 mm). The effect of straw length on earthworm utilisation was tested by earthworm incubation experiments in plastic bags, where removal from the soil surface was measured over a period of 8 weeks in monocultures and mixed species treatments. Litter removal was investigated by mass depletion over time and depth of incorporation. Choice chambers were used to quantify straw selection and removal at micro particle size. Expt 1 showed straw removal (63 ± 6 %) was significantly higher with a <em>L. terrestris</em> monoculture and 1 cm length. The largest masses of straw were incorporated at 0–60 mm depth of soil. There was no evidence to support a facilitation effect of <em>L. terrestris</em> on <em>A. caliginosa</em>, and increased earthworm mortality was detected in mixed species treatments. The choice chambers of Expt 2 indicated a preference for 1 mm particle size by both earthworm species with a more rapid use by <em>L. terrestris</em> than <em>A. caliginosa</em>. These experiments highlight how retention of straw residues on the field, linked with tillage practices and further straw management post-harvest could have significant implications for plant protection and earthworm populations.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"128 ","pages":"Article 103799"},"PeriodicalIF":3.3,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of forest management intensity on soil microbial diversity and community assembly in Carya cathayensis plantations","authors":"Wei Fang ,&nbsp;jing Gao ,&nbsp;Shuai Shao ,&nbsp;Chenfei Liang ,&nbsp;Junhui Chen ,&nbsp;Hua Qin ,&nbsp;Qiufang Xu","doi":"10.1016/j.ejsobi.2025.103798","DOIUrl":"10.1016/j.ejsobi.2025.103798","url":null,"abstract":"<div><div>Intensive management of <em>Carya cathayensis</em> (<em>C. cathayensis</em>) plantations has been linked to soil degradation and increased disease incidence, yet the underlying shifts in rhizosphere microbial communities remain poorly understood. We compared rhizosphere soils from non-managed forest(NF), reduced-management forest(RF), and intensive-management forest(IF) stands across two towns in Zhejiang, China. With increasing management intensity, soil fertility and enzyme activities declined, bacterial diversity and network stability decreased, whereas fungal diversity tended to increase. Bacterial community assembly was predominantly deterministic and became more so under intensive management, whereas fungal assembly remained largely stochastic. Management intensity did not directly regulate microbial assembly; instead, soil chemical properties and enzyme activities mediated these patterns. Several microbial taxa responded strongly to management intensity and were significantly correlated with community assembly processes. These findings reveal consistent associations between management intensity and rhizosphere microbial patterns across two landscapes, despite potential site-related variation. Reducing management intensity favours microbial network complexity and stability, offering microbiome-based avenues for sustainable <em>C. cathayensis</em> forestry.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"128 ","pages":"Article 103798"},"PeriodicalIF":3.3,"publicationDate":"2025-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term effect of changing water regimes on the soil nematode community in rice-duckweed system under water-saving irrigation","authors":"Zeeshan Ahmed ,&nbsp;Junzeng Xu ,&nbsp;Weixuan Liu ,&nbsp;Xiaoyin Liu ,&nbsp;Yawei Li ,&nbsp;Hang Guo ,&nbsp;Shengyu Chen","doi":"10.1016/j.ejsobi.2025.103791","DOIUrl":"10.1016/j.ejsobi.2025.103791","url":null,"abstract":"<div><div>Rice agroecosystems have garnered global attention owing to a unique transition from traditional flooding to water-saving practices, such as alternate wetting and drying (AWD) irrigation, which features a dual-habitat ecosystem with high biodiversity throughout the growing period. Nematodes' taxonomic bioindication potential could reveal perturbations in the soil environment and changes in food web status under AWD. However, the influence of changing dual modes (dry and wet habitats) with duckweed (D) on nematodes was often overlooked. Therefore, a field experiment was conducted to quantify the impact of flooding irrigation, and alternate wetting and drying irrigation on nematode abundance across four treatments: flooding irrigation with (FI + D) and without duckweed (FI), alternate wetting and drying irrigation with (AWD + D) and without (AWD) duckweed. For flooding irrigation, soil samples were taken at tillering, pre-mid-season drainage (MD), and at flowering post-MD. Similarly, for alternate wetting and drying irrigation at tillering and flowering for respective dry and wet cycles. The first AWD dry-cycle with duckweed (AWD + D) significantly reduced nematode abundance compared to the wet cycle (AWD + D) (<em>P</em> &lt; 0.033). In FI, MD significantly reduced nematode abundance (<em>P</em> &lt; 0.007) compared to FI + D. Factor prediction analytics showed that pH in the FI post-MD and <span><math><mrow><mo>[</mo><mrow><msubsup><mtext>NH</mtext><mn>4</mn><mo>+</mo></msubsup><mo>−</mo><mi>N</mi></mrow><mo>]</mo></mrow></math></span> in flooded and AWD-integrated treatments were the most influential abiotic drivers governing nematode taxonomic and functional diversity. AWD dry-wet cycles with duckweed, fuel colonizer CP (1–2, r-strategists) nematodes, indicating better soil health and higher microbial turnover than without duckweed. The AWD drying cycle with duckweed produced more mature, fertile soils with balanced bacterivores/fungivores and moderate C:N ratios, and demonstrated suppressive properties, which further seek an investigation into the link between soil biochemical enrichment from decomposing duckweed.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"128 ","pages":"Article 103791"},"PeriodicalIF":3.3,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145665357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of vermicompost from urban organic waste leads to a sustainable increase of earthworm abundance: a three-year study in a crop field converting to organic farming","authors":"Vincent Ducasse ,&nbsp;Vincent Tolon ,&nbsp;Apolline Auclerc ,&nbsp;Line Capowiez ,&nbsp;Joséphine Peigné ,&nbsp;Yvan Capowiez","doi":"10.1016/j.ejsobi.2025.103789","DOIUrl":"10.1016/j.ejsobi.2025.103789","url":null,"abstract":"<div><div>The management of organic matter in sustainable agriculture remains challenging in the 21st century. For farms located close to large cities, the organic fraction of municipal solid waste (OFMSW) is a potential resource to improve soil biological quality. However, this fraction can be processed in very different ways: compost, digestate, or vermicompost. We carried out a three-year trial to compare the effects on earthworm communities of two successive applications of such products at two doses (except for digestate) in a field crop recently converted to organic farming and no tillage. The abandonment of tillage led to a marked increase in the soil bulk density (from 1.25 to 1.45 g cm⁻³) and an associated and continuous decrease in the number of juvenile earthworms in all plots. For abundance of adult earthworms, compost had no effect, whereas digestate led to a large and rapid increase immediately after each application. By contrast, vermicompost steadily increased adult earthworm abundance (+33 to + 90 % compared to the control, depending on the sampling dates). Similar results were observed for earthworm biomass. In this trial, highly dominated by endogeics (80 % of the abundance), no difference between treatments was observed for the temporal variation of each species. However, <em>Aporrectodea caliginosa</em> abundance greatly increased after the first application, whereas <em>Aporrectodea icterica</em> only increased after the second application. The impact of vermicompost on earthworm communities has never been studied under field conditions so far, but this product seems promising for farmers who may seek to benefit from the services provided by earthworms.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103789"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leaf litter quantity shapes oribatid mite assemblages five years following forestry interventions in a European deciduous forest","authors":"Veronika Gergócs-Winkler ,&nbsp;Norbert Flórián ,&nbsp;Bence Kovács ,&nbsp;Réka Aszalós ,&nbsp;András Bidló ,&nbsp;Péter Ódor","doi":"10.1016/j.ejsobi.2025.103790","DOIUrl":"10.1016/j.ejsobi.2025.103790","url":null,"abstract":"<div><div>Continuous cover forestry is widely regarded as a more ecologically sustainable alternative to traditional rotation-based systems in Europe. However, the short- and long-term impacts of different forestry interventions on soil biota remain poorly understood. We investigated the effects of four forest management treatments on soil-dwelling oribatid mites in a European oak–hornbeam forest, five years post-intervention. Assemblages in clear-cut, gap-cut, preparation-cut, and retention tree group plots were compared with controls. Soil fauna were sampled seasonally; climatic conditions were continuously monitored, and vegetation and soil properties measured annually. Mites were identified to species level, and both taxonomic and trait-based community metrics were analysed to assess ecological responses to the treatments. Microclimatic and soil variables did not differ significantly among treatments, but leaf litter quantity was highest in the control, preparation-cutting, and retention tree group plots. Oribatid mite density and species richness were lowest in clear-cutting and gap-cutting plots, highest in the control, and intermediate in the preparation-cutting and retention tree group plots. The most abundant species, mainly from the family Oppiidae, had reduced densities in the more intensively disturbed treatments, contributing to higher evenness in those plots. Less disturbed habitats were dominated by omnivorous, predatory, and scavenger species, with higher proportions of parthenogenetic individuals. In contrast, sexual and predominantly detritivorous species were more prevalent in the clear-cutting and gap-cutting plots. Furthermore, seasonal variation in species composition was more pronounced in these disturbed plots, whereas species composition remained more stable and homogeneous in the control plots. In conclusion, higher amount of leaf litter in less disturbed plots supported the dominance of parthenogenetic and omnivorous species. Oribatid mites proved to be sensitive indicators of long-term ecological effects, highlighting the lasting impact of forestry interventions in oak–hornbeam forests, even five years after disturbance.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103790"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial necromass carbon mediates nonlinear soil carbon response to nitrogen application in the greenhouse vegetable production system","authors":"Zhou Jia ,&nbsp;Lifeng Zhang ,&nbsp;Chengzhang Wang ,&nbsp;Longgang Jiang ,&nbsp;Erxiong Zhu ,&nbsp;Meng Li ,&nbsp;Ruonan Li ,&nbsp;Li Guo ,&nbsp;Yihong Li ,&nbsp;Liying Wang ,&nbsp;Jianshuo Shi","doi":"10.1016/j.ejsobi.2025.103788","DOIUrl":"10.1016/j.ejsobi.2025.103788","url":null,"abstract":"<div><div>Soil organic carbon (SOC) constitutes the largest terrestrial C reservoir, playing a pivotal role in global C cycling. Nitrogen (N) fertilization has been widely recognized as a major driver of SOC transformation and accumulation. However, the specific mechanistic pathways by which long-term differential N application rates modulate microbially derived SOC components and thereby influence SOC persistence in greenhouse vegetable production (GVP) systems remain to be elucidated. We implemented a 12-year field trial assessing five chemical N application rates (cucumber/tomato season: 0/0 (N0), 200/200 (N200), 300/225 (N300), 600/450 (N600), and 900/675 (N900) kg N ha⁻¹ yr⁻¹) in an intensive GVP system. This study integrates measurements of microbial necromass carbon (MNC), kinetics of C- and N-acquiring hydrolases, soil chemical properties and phospholipid fatty acids (PLFAs) to unravel the impacts of N application rates on SOC dynamics and persistence. Our results demonstrate that MNC constitutes 54.5–77.0 % of SOC across all N addition treatments, with fungal necromass C (FNC) averaging 4.40 times higher than bacterial necromass C (BNC). Interestingly, our findings revealed that the MNC/SOC was nonlinearly correlated with elevated N addition rates. Specifically, the BNC/SOC peaked under the N600 treatment, whereas the FNC/SOC was maximized under the N300 treatment and then showed a downward trend. Hierarchical partitioning results indicated that enzymatic kinetics as the predominant regulator of both MNC/SOC and FNC/SOC, whereas soil nutrient attributes exerted stronger control over BNC/SOC. Moreover, the PLS-PM results identified soil properties (e.g., Total N, C:N, NO₃⁻-N, dissolved organic C, pH, and electrical conductivity) as the primary positive regulators of BNC/SOC. However, FNC/SOC and MNC/SOC were predominantly driven by the kinetics of C- (α-glucosidase, β-glucosidase, β-xylosidase) and N-acquiring (N-acetyl-glucosaminidase) enzymes. Therefore, these drivers exhibited diametrically opposing effects on BNC/SOC relative to FNC/SOC (MNC/SOC). Our findings establish that optimized N management is critical for sustaining SOC sequestration in GVP systems, providing a mechanistic basis for linking N application to microbial-mediated C stabilization in the intensive agriculture systems.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103788"},"PeriodicalIF":3.3,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145575912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low mycovirus presence in soil saprotrophic fungi in Dutch sandy soils","authors":"Ruiqi Wang ,&nbsp;Hazal Kandemir ,&nbsp;Jing Zhang ,&nbsp;T. Martijn Bezemer ,&nbsp;Peter M. van Bodegom ,&nbsp;S. Emilia Hannula","doi":"10.1016/j.ejsobi.2025.103787","DOIUrl":"10.1016/j.ejsobi.2025.103787","url":null,"abstract":"<div><div>Saprotrophic fungi play a fundamental role in soil ecosystems as primary decomposers, driving nutrient and carbon turnover on a global scale. Mycoviruses are considered widespread and can affect saprotrophic fungi by altering their properties, such as growth rate, stress resistance, and metabolite production. To advance the understanding of the prevalence and diversity of mycoviruses of soil saprotrophic fungi, we conducted parallel mycovirus screening in 28 saprotrophic fungi representing major groups of soil fungi and concomitantly explored soil metatranscriptomic data. <em>De novo</em> assembly of RNA-sequencing data uncovered two viruses from two fungal strains. One of these viruses, a ssRNA virus from <em>Mucor</em> sp., appears to represent a putative novel species. Analysis of eukaryotic RNA viruses in soil metatranscriptomes indicated that even though fungal and oomycete hosts made up 39.6 % of the classified eukaryotic viruses, there was no saprotrophic host assignment. Both methods showed that there were few mycoviruses in the Dutch sandy soils with different land uses. This led to the conclusion that mycoviruses affecting saprotrophic fungi may either be rare in these types of soils or more difficult to detect compared to other fungal groups. These results offer new insights into the ecological dynamics and viral associations of soil saprotrophic fungi, highlighting the need for broader sampling and improved approaches to assess mycovirus diversity and its potential ecological significance.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103787"},"PeriodicalIF":3.3,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145575906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}