European Journal of Soil Biology最新文献

Functional divergence in the rhizosphere microbiome of Codonopsis pilosula drives a soil carbon allocation trade-off 党参根际微生物组功能分化驱动土壤碳分配权衡

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2026-03-01 Epub Date: 2026-02-23 DOI: 10.1016/j.ejsobi.2026.103811

Wen Luo , Yali Guo , Zhihu Wang , Wenying Wang , Yuanli Li , Lili Liu , Wenjuan Wang , Ming Luo , Yonggang Wang

Rhizosphere microorganisms play pivotal roles in soil organic carbon dynamics; yet, their relationship with soil carbon cycling remains unclear under plant intraspecific variation, particularly for medicinal species with distinct metabolic traits. Here, we investigate how six varieties of Codonopsis pilosula (BT, CD, WD, WY1, WY3, WY4) influence the trade-off between soil organic matter (SOM) and microbial biomass carbon (MBC) through modulation of rhizosphere microbial communities. The results showed that a significant negative correlation was observed between SOM and MBC across varieties (P = 0.0025). The BT variety exhibited a rapid carbon turnover phenotype, marked by low SOM, high MBC, and enhanced peroxidase activity. In contrast, WD and WY3 adopted a carbon-accumulating strategy, sustaining high SOM with moderate to low MBC. Plant variety emerged as the dominant factor structuring rhizosphere bacterial and fungal communities. The BT variety specifically enriched taxa involved in recalcitrant carbon degradation, such as Nitrospira and Chryseolinea. Functional prediction further revealed enrichment of nitrification and lignin degradation pathways in BT microbiomes, whereas denitrification was prominent in WY4. Network analyses underscored strong associations among SOM, MBC, and carbon-cycling enzymes with microbial network modules, suggesting that environmental factors modulate carbon processes via microbiome interactions. Our findings unveil a mechanism by which plant genetic variation mediates soil carbon allocation through rhizosphere community restructuring, providing a foundation for genotype-specific breeding and microbiome management to optimize soil carbon sequestration.

根际微生物在土壤有机碳动态中起关键作用；然而，它们与土壤碳循环的关系在植物种内变异下仍不清楚，特别是对于具有不同代谢性状的药用物种。本文研究了6个党参品种（BT、CD、WD、WY1、WY3、WY4）如何通过调节根际微生物群落对土壤有机质（SOM）和微生物生物量碳（MBC）平衡的影响。结果表明，不同品种间SOM与MBC呈显著负相关（P = 0.0025）。BT品种表现出快速的碳转换表型，表现为低SOM、高MBC和过氧化物酶活性增强。相比之下，WD和WY3采用碳积累策略，保持高SOM和中低MBC。植物品种是构成根际细菌和真菌群落的主导因素。BT品种特别富集了参与顽固性碳降解的分类群，如硝基螺旋藻和chrysseolinea。功能预测进一步揭示了BT菌群中硝化和木质素降解途径的富集，而反硝化作用在WY4中突出。网络分析强调了SOM、MBC和碳循环酶与微生物网络模块之间的强烈关联，表明环境因素通过微生物组相互作用调节碳过程。本研究揭示了植物遗传变异通过根际群落重组介导土壤碳分配的机制，为基因型特异性育种和微生物组管理优化土壤固碳提供了基础。

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引用次数: 0

Nematode predation mitigates microbial competition to sustain the plant growth-promoting effect of Streptomyces 线虫的捕食减轻了微生物的竞争，以维持链霉菌促进植物生长的作用

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2026-03-01 Epub Date: 2026-01-23 DOI: 10.1016/j.ejsobi.2026.103806

Ke-Fan Huo , Jing-Nan Zhang , Elly Morriën , Ping Liu , Neil B. McLaughlin , Kai-Hong Bai , Zhen-Long Wang , Shi-Xiu Zhang , Lu-Jun Li

Streptomyces, a well-known plant growth-promoting rhizobacterium (PGPR), often shows inconsistent efficacy in soil due to poorly understood interactions with native microbes and soil fauna. This study used factorial pot experiments to investigate how microbial competition and nematode predation influence the growth-promoting effect of Streptomyces on Arabidopsis thaliana (A. thaliana), through co-inoculation with indigenous soil microbes or/and nematodes. Compared to inoculation with Streptomyces alone, co-inoculation with either indigenous microbes or nematodes significantly reduced the aboveground dry biomass of A. thaliana, and Streptomyces relative abundance declined by 61.29 % and 79.68 %, respectively. Sequencing showed that Streptomyces introduction altered the indigenous resident community and significantly increased the abundance of functional genes for nutrient competition and antibiotic synthesis, resulting in strong competitive exclusion that impaired its growth-promoting effect. Nematode identification and feeding assays revealed that over 90 % of the nematode community were bacterivores grazing on Streptomyces spores, thereby preventing population recovery and growth-promoting function. However, when Streptomyces, microbes, and nematodes coexisted, the plant growth promotion was restored. This recovery was primarily driven by nematodes selectively grazing on competing taxa, reshaping the resident community and alleviating competitive constraints on Streptomyces, thereby sustaining its plant growth-promoting effect. These findings highlight trophic buffering as a key mechanism maintaining PGPR function in complex soils, which informs future strategies that design and apply PGPR inoculants around multitrophic interactions to achieve more stable field performance.

链霉菌（Streptomyces）是一种众所周知的促进植物生长的根瘤菌（PGPR），由于对其与本地微生物和土壤动物的相互作用知之甚少，其在土壤中的作用往往不一致。本研究采用因子盆栽试验，通过与当地土壤微生物或/和线虫共接种，研究链霉菌对拟南芥（拟南芥）的促生长作用是如何影响微生物竞争和线虫捕食的。与单独接种链霉菌相比，与本地微生物或线虫共接种均显著降低拟南螺旋藻地上干生物量，链霉菌相对丰度分别下降61.29%和79.68%。测序结果表明，链霉菌的引入改变了当地居民群落，显著增加了营养竞争和抗生素合成功能基因的丰度，导致强烈的竞争排斥，削弱了其促进生长的作用。线虫鉴定和取食实验表明，90%以上的线虫群落以链霉菌孢子为食菌，从而阻碍了种群恢复和促进生长的功能。然而，当链霉菌、微生物和线虫共存时，植物生长促进恢复。这种恢复主要是由于线虫选择性地放牧竞争类群，重塑了居民群落，减轻了链霉菌的竞争限制，从而维持了其促进植物生长的作用。这些发现强调了营养缓冲是在复杂土壤中维持PGPR功能的关键机制，这为未来设计和应用PGPR接种剂的策略提供了信息，以实现更稳定的田间性能。

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引用次数: 0

Corrigendum to “Carbon and energy utilization in microbial cell extracts from soil” [Eur. J. Soil Biol. 124 (2025) 103713] “土壤中微生物细胞提取物的碳和能量利用”的勘误[欧洲]。[j] .水土保持学报，2016,27(5):377 - 378。

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2026-03-01 Epub Date: 2025-12-29 DOI: 10.1016/j.ejsobi.2025.103801

Milan Varsadiya , Fatemeh Dehghani , Shiyue Yang , Felix Beulig , Evgenia Blagodatskaya , Thomas Maskow , Dimitri V. Meier , Tillmann Lueders

引用次数: 0

Responses of the earthworms Lumbricus terrestris and Aporrectodea caliginosa to wheat straw provision across a range of residue sizes 不同秸秆大小下地蚓和土石蚓对麦秸供应的响应

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2026-03-01 Epub Date: 2025-12-17 DOI: 10.1016/j.ejsobi.2025.103799

Peter Bentley, Kevin R. Butt

Earthworm mediated incorporation of soil surface applied crop residues could provide benefits to belowground ecosystem services, such as an increased rate of soil formation and carbon sequestration. In addition, increased soil organic matter within the upper soil profile can increase food availability for other soil fauna and microorganisms, with potential benefits for soil structure and health. Previous research has assessed the potential mass of surface applied organic matter that can be assimilated by earthworms; however, particle size of material may limit the rate of bioturbation and influence earthworm function and behaviour. The aims of the present study were to investigate the preference and utilisation of wheat (Triticum aestivum) straw residues at different particle sizes by two common, temperate earthworm species, Lumbricus terrestris and Aporrectodea caliginosa. These were addressed within controlled laboratory experiments, where two different scales were tested: Expt 1; with 3 modal straw lengths, as determined from the field post-harvest (40, 20 and 1 cm); and Expt 2; micro particle sizes (1 cm and 1 mm). The effect of straw length on earthworm utilisation was tested by earthworm incubation experiments in plastic bags, where removal from the soil surface was measured over a period of 8 weeks in monocultures and mixed species treatments. Litter removal was investigated by mass depletion over time and depth of incorporation. Choice chambers were used to quantify straw selection and removal at micro particle size. Expt 1 showed straw removal (63 ± 6 %) was significantly higher with a L. terrestris monoculture and 1 cm length. The largest masses of straw were incorporated at 0–60 mm depth of soil. There was no evidence to support a facilitation effect of L. terrestris on A. caliginosa, and increased earthworm mortality was detected in mixed species treatments. The choice chambers of Expt 2 indicated a preference for 1 mm particle size by both earthworm species with a more rapid use by L. terrestris than A. caliginosa. These experiments highlight how retention of straw residues on the field, linked with tillage practices and further straw management post-harvest could have significant implications for plant protection and earthworm populations.

蚯蚓介导的地表作物残茬土壤掺入可以为地下生态系统服务提供益处，例如增加土壤形成和碳固存的速度。此外，上层土壤剖面中土壤有机质的增加可以增加其他土壤动物和微生物的食物供应，对土壤结构和健康有潜在的好处。以前的研究已经评估了蚯蚓可以吸收的表面施用有机物的潜在质量；然而，材料的粒径可能会限制生物扰动的速度，并影响蚯蚓的功能和行为。本研究的目的是研究两种常见的温带蚯蚓蚓蚓对不同粒径的小麦秸秆秸秆秸秆的偏好和利用。这些都是在受控实验室实验中解决的，其中测试了两种不同的量表：出口1；采用3种秸秆模态长度，由收获后田间测定（40、20和1厘米）；和出口2；微颗粒尺寸（1厘米和1毫米）。通过在塑料袋中进行蚯蚓孵化实验，测试了秸秆长度对蚯蚓利用的影响，在单一栽培和混合物种处理中，在8周的时间内测量了蚯蚓从土壤表面的去除量。通过随时间和掺入深度的质量损耗来研究垃圾去除。选择室用于量化秸秆在微观粒度上的选择和去除。实验1显示，1 cm长度的单株地屈草秸秆去除率（63±6%）显著高于单株地屈草。在0-60 mm深度的土壤中加入了最大质量的稻草。没有证据支持土生l.s terrestris对caliginosa的促进作用，并且在混合种处理中发现蚯蚓死亡率增加。结果表明，两种蚯蚓均对粒径为1 mm的蚯蚓有较大的偏好，其中陆生蚯蚓对1 mm粒径蚯蚓的利用速度较快。这些实验突出了秸秆秸秆在田间的保留，与耕作方法和收获后进一步的秸秆管理相联系，如何对植物保护和蚯蚓种群产生重大影响。

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引用次数: 0

Soil microbial beta diversity is correlated positively with ecosystem multifunctionality of alpine meadows on the Tibetan plateau 青藏高原高寒草甸土壤微生物多样性与生态系统多功能性呈正相关

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2026-03-01 Epub Date: 2026-01-31 DOI: 10.1016/j.ejsobi.2026.103809

Lu-ming Ding , Tian-yang Zhou , Miao Yang , Abraham Allan Degen , Chang-ting Wang

Alpine grasslands are characterized by high environmental heterogeneity, with multiple ecological processes operating simultaneously. Capturing ecosystem functioning, therefore, requires approaches that consider several functions together rather than relying on single indicators. Ecosystem multifunctionality (EMF) provides a framework to integrate this functional diversity across spatial scales, while acknowledging that its interpretation depends on the functions considered. Generally, soil microorganisms are regarded as the main drivers of grassland EMF, but the relations between them in the alpine meadow ecosystem remain unknown. The main aim of this study was to fill this gap by examining the impacts of soil bacteria and fungi on EMF. From a field study of 90 sites across the southeastern part of the Tibetan Plateau, soil bacterial and fungal β diversities were correlated positively with EMF, and the relationships were mediated mainly by soil pH. The soil fungal, but not bacterial, community network stability also correlated positively with EMF. In addition, plant Pielou evenness and soil bacterial Shannon diversity were correlated negatively with EMF, while the Shannon diversities of soil fungi and plant species were not correlated with EMF. The current results emphasized that: 1) soil bacteria and fungi had different impacts on EMF; and 2) bacterial and fungal β diversities were important drivers of EMF in alpine meadows of the Tibetan plateau.

高寒草原具有高度的环境异质性，多种生态过程同时发生。因此，捕捉生态系统功能需要综合考虑多种功能的方法，而不是依赖单一指标。生态系统多功能（EMF）提供了一个跨空间尺度整合这种功能多样性的框架，同时承认其解释取决于所考虑的功能。土壤微生物通常被认为是草地电磁场的主要驱动因素，但在高寒草甸生态系统中，土壤微生物与草地电磁场的关系尚不清楚。这项研究的主要目的是通过研究土壤细菌和真菌对电磁场的影响来填补这一空白。通过对青藏高原东南部90个样点的野外调查，发现土壤细菌和真菌β多样性与EMF呈显著正相关，且主要受土壤ph的调节。土壤真菌群落网络稳定性与EMF呈显著正相关，而细菌群落网络稳定性与EMF无显著正相关。此外，植物Pielou均匀度和土壤细菌Shannon多样性与EMF呈负相关，而土壤真菌和植物物种的Shannon多样性与EMF不相关。目前的研究结果强调：1)土壤细菌和真菌对电磁场有不同的影响；2)细菌和真菌β多样性是青藏高原高寒草甸电磁场的重要驱动因子。

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引用次数: 0

Bio-organic fertilization promotes soil nutrient cycling and reduces CO2 emissions via regulating bacterial communities in vegetable production system 生物有机肥通过调节蔬菜生产系统中的细菌群落，促进土壤养分循环，减少二氧化碳排放

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2026-03-01 Epub Date: 2026-02-04 DOI: 10.1016/j.ejsobi.2026.103807

Lijun Ren , Lili Dong , Yanyu Han , Jiaqi Li , Qingfeng Fan , Dan Wei , Hongtao Zou , Yulong Zhang

Organic fertilizer substitution plays a crucial role in improving soil quality and reducing carbon emissions. However, the microbial mechanisms underlying effects on soil nutrient cycling and carbon emissions, especially in vegetable cultivation systems, remain poorly understood. A five-year field experiment (2019–2023) was conducted to evaluate the effects of fertilization strategies, including no fertilization (CK), inorganic fertilization (CF), bio-organic fertilization (OF), and their combination (COF). The impact of bio-organic fertilization on soil nutrient cycling, carbon emissions, and carbon-cycling microbial communities was analyzed. The highest multi-nutrient cycling index was observed in COF treatment. Compared to the CF treatment, the bio-organic fertilization treatments (OF, 35.9 %; COF, 12.1 %) significantly (P<0.05) reduced soil CO₂ emissions. Bacterial diversity (Chao1 index and Shannon index) in the OF and COF treatments was significantly (P<0.05) higher than in the CF treatment, whereas fungal diversity (Shannon index) showed the opposite trend. Bacterial diversity played a crucial role in regulating soil nutrient status, as bacterial co-occurrence network exhibited a highly significant positive correlation with the multinutrient cycling index. Bio-organic fertilizer enhanced the activity of genes related to multiple systems (pccA), rTCA cycle (icd), reductive acetyl-CoA pathway (cooC), and Calvin cycle (cbbL/R), thereby promoting the conversion of CO₂ into stable organic compounds. Furthermore, structural equation modeling confirmed that soil carbon fixation genes, bacterial diversity, and network stability were key factors influencing CO₂ emissions. Overall, from a long-term perspective, organic fertilizer substitution can mitigate carbon emissions and promote nutrient cycling in greenhouse vegetable cultivation systems, representing a greener and more sustainable agricultural approach.

有机肥替代对改善土壤质量和减少碳排放具有至关重要的作用。然而，微生物对土壤养分循环和碳排放的潜在影响机制，特别是在蔬菜栽培系统中，仍然知之甚少。为评价不施肥（CK）、无机施肥（CF）、生物有机肥（of）及其组合施肥（COF）的效果，进行了为期5年（2019-2023）的田间试验。分析了生物有机肥对土壤养分循环、碳排放和碳循环微生物群落的影响。COF处理的多养分循环指数最高。与CF处理相比，生物有机肥处理（OF, 35.9%； COF, 12.1%）显著降低了土壤CO2排放（P<0.05）。有机肥和有机肥处理的细菌多样性（Chao1指数和Shannon指数）显著高于有机肥处理（P<0.05），真菌多样性（Shannon指数）则相反。细菌多样性对土壤养分状况起着至关重要的调节作用，细菌共生网络与多养分循环指数呈极显著正相关。生物有机肥增强了多系统（pccA）、rTCA循环（icd）、还原性乙酰辅酶a途径（cooC）和卡尔文循环（cbbL/R）相关基因的活性，从而促进了CO2向稳定有机化合物的转化。此外，结构方程模型证实了土壤固碳基因、细菌多样性和网络稳定性是影响CO2排放的关键因素。总体而言，从长期来看，有机肥替代可以减少温室蔬菜种植系统的碳排放，促进养分循环，是一种更绿色、更可持续的农业方式。

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引用次数: 0

Cattle excreta on Lotus tenuis-promoted grasslands: Exploring soil microbiome and nitrous oxide emissions in the Salado River Basin, Argentina 荷花种植草地上的牛排泄物：探索阿根廷萨拉多河流域土壤微生物群和一氧化二氮排放

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2026-03-01 Epub Date: 2026-01-24 DOI: 10.1016/j.ejsobi.2026.103805

Amira Susana del V. Nieva , Mónica G. Pérez , Cristian J. Antonelli , Alejandro O. Costantini , Oscar A. Ruiz

Cattle production generates inputs such as urine and dung that increase soil microbial diversity by adding organic matter and nitrogen. Legume pastures, such as Lotus tenuis improve soil quality by incorporating nitrogen, influencing microbial communities, and contributing to nitrous oxide (N₂O) emissions. This study aimed to investigate the impact of livestock and pasture systems on soil microbiomes and N₂O emissions within grassland ecosystems. We analysed microbial communities in soils of natural and L. tenuis-promoted grasslands, using Next Generation Sequencing (NGS) and simulated urine and dung depositions in field mesocosms. The results revealed significant differences in microbial diversity between natural and L. tenuis-promoted grasslands across cattle input treatments. Biomarker analysis identified distinct phyla in each type of excreta and grassland system. In natural grasslands, Proteobacteria and Actinobacteriota were prevalent with urine, whereas Acidobacteriota and Verrucomicrobiota characterised the soils promoted by L. tenuis. The indicators of the dung-treated soils were Pseudonocardia, Flavobacterium, NP-4, Adhaeribacter, Pseudoxanthomonas, and Novosphingobium, while Brevundimonas, Defluviicoccus, Sphingomonas, Sphingomicrobium, REEP01, Chthoniobacter, Hyphomicrobium, and PSRF01 were microbial indicators of the urine-treated soils. The N₂O emissions were significantly lower in fields with L. tenuis and urine addition compared to natural grasslands under similar conditions, with genera like Brevundimonas, Hyphomicrobium, and Nitrosocosmicus positively correlated with emissions. These findings underscore the role of pasture composition in shaping soil microbiomes and highlight the benefits of legumes, such as L. tenuis, in reducing N₂O emissions, providing an alternative for more sustainable livestock management practices.

牛生产产生的尿液和粪便等投入物通过添加有机物和氮来增加土壤微生物的多样性。豆科牧草，如荷叶草，通过吸收氮、影响微生物群落和促进一氧化二氮（N2O）排放来改善土壤质量。本研究旨在探讨畜牧场系统对草地生态系统土壤微生物组和N2O排放的影响。利用新一代测序技术（NGS）和模拟野外中生态系统的尿液和粪便沉积，对天然草地和羊草种植草地土壤中的微生物群落进行了分析。结果表明，在不同的牛投入处理下，自然草地和羊草促进草地的微生物多样性存在显著差异。生物标志物分析在每种类型的排泄物和草地系统中鉴定出不同的门。在天然草地上，尿中主要存在变形菌群和放线菌群，而羊草菌促进土壤中主要存在酸性菌群和Verrucomicrobiota。粪处理土壤的微生物指标为pseudondimonas、Flavobacterium、NP-4、Adhaeribacter、Pseudoxanthomonas和Novosphingobium；尿处理土壤的微生物指标为Brevundimonas、defluviiccoccus、Sphingomonas、Sphingomicrobium、REEP01、Chthoniobacter、hyphomicroum和PSRF01。在相似条件下，与天然草地相比，添加了羊草和尿液的农田N2O排放量显著降低，Brevundimonas、菌丝微生物和亚硝基菌等属与排放量呈正相关。这些发现强调了牧草组成在塑造土壤微生物组中的作用，并强调了豆科植物（如L. tenuis）在减少一氧化二氮排放方面的益处，为更可持续的牲畜管理实践提供了一种替代方案。

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引用次数: 0

Effects of grassland enclosure on phosphorus bioavailability and microbial nutrient limitations in the karst region of southwest China 草地围封对西南喀斯特地区磷生物有效性和微生物养分限制的影响

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2026-03-01 Epub Date: 2026-01-30 DOI: 10.1016/j.ejsobi.2026.103808

Wangjun Li , Xiaolong Bai , Shun Zou , Bin He , Yurong Yang

Phosphorus (P) availability is a critical factor limiting the restoration of degraded ecosystems in nutrient-poor karst regions. Enclosure has been considered an effective strategy to restore degraded grasslands. However, the mechanisms through which grazing enclosure affects soil P fractions and microbial nutrient limitations in these fragile regions remain unclear. We investigated soil P fractions (resin-P, NaHCO₃–P, NaOH–P, HCl–P, and residual-P) and microbial nutrient limitations along a subalpine grassland restoration chronosequence (3, 9, 15, 23 years enclosure) in the karst area of southwest China. The nutrient limitations of microbes were quantified by enzymatic vector analysis. The results showed that vector length initially increased, then declined, while the angle decreased with the restoration stage, indicating a microbial community shift from P to nitrogen (N) limitation. Residual-P was the dominant fraction, followed by HCl-P, highlighting stable P pool prevalence. Despite this, over the restoration chronosequence, resin-P content increased, and residual-P decreased progressively, indicating enhanced P bioavailability. Random forest model and structural equation model identified soil pH, extracellular enzymes related to carbon (C) and P cycle, and the relative abundance of microbial core taxa (i.e., Actinobacteriota, Glomeromycota) as primary drivers of P availability and microbial nutrient limitations. Overall, our results highlight that long-term enclosure is an effective management strategy for improving P availability and alleviating microbial P limitation in karst grasslands, and also emphasize the importance of specific microbial taxa (high relative abundance and high connectivity within the co-occurrence network) for soil nutrient cycling during grassland restoration.

在营养贫乏的喀斯特地区，磷有效性是限制退化生态系统恢复的关键因素。围护被认为是恢复退化草原的有效策略。然而，放牧对这些脆弱地区土壤磷组分和微生物养分限制的影响机制尚不清楚。研究了西南喀斯特地区亚高山草地恢复时间序列（3、9、15、23年）土壤P组分（树脂P、NaHCO3-P、NaOH-P、HCl-P和残余P）和微生物养分限制。利用酶载体分析法定量微生物的营养限制。结果表明，随着恢复阶段的延长，载体长度呈现先增大后减小的趋势，载体长度角度呈减小趋势，表明微生物群落由磷限制向氮限制转变。残差P占主导地位，其次是HCl-P，表明P池患病率稳定。尽管如此，在恢复的时间序列中，树脂磷含量逐渐增加，残余磷逐渐减少，表明磷的生物利用度增强。随机森林模型和结构方程模型表明，土壤pH、与碳(C)和磷循环相关的胞外酶以及核心微生物类群（放线菌群、肾小球菌群）的相对丰度是磷有效性和微生物养分限制的主要驱动因素。综上所述，长期围封是改善喀斯特草原磷有效性和缓解微生物磷限制的有效管理策略，同时也强调了特定微生物类群（高相对丰度和高共现网络内的连连性）对草地恢复过程中土壤养分循环的重要性。

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引用次数: 0

Effects of forest management intensity on soil microbial diversity and community assembly in Carya cathayensis plantations 森林经营强度对山核桃人工林土壤微生物多样性和群落聚集的影响

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2026-03-01 Epub Date: 2025-12-14 DOI: 10.1016/j.ejsobi.2025.103798

Wei Fang , jing Gao , Shuai Shao , Chenfei Liang , Junhui Chen , Hua Qin , Qiufang Xu

Intensive management of Carya cathayensis (C. cathayensis) plantations has been linked to soil degradation and increased disease incidence, yet the underlying shifts in rhizosphere microbial communities remain poorly understood. We compared rhizosphere soils from non-managed forest(NF), reduced-management forest(RF), and intensive-management forest(IF) stands across two towns in Zhejiang, China. With increasing management intensity, soil fertility and enzyme activities declined, bacterial diversity and network stability decreased, whereas fungal diversity tended to increase. Bacterial community assembly was predominantly deterministic and became more so under intensive management, whereas fungal assembly remained largely stochastic. Management intensity did not directly regulate microbial assembly; instead, soil chemical properties and enzyme activities mediated these patterns. Several microbial taxa responded strongly to management intensity and were significantly correlated with community assembly processes. These findings reveal consistent associations between management intensity and rhizosphere microbial patterns across two landscapes, despite potential site-related variation. Reducing management intensity favours microbial network complexity and stability, offering microbiome-based avenues for sustainable C. cathayensis forestry.

山核桃（C. cathayensis）人工林的集约化管理与土壤退化和疾病发病率增加有关，但根际微生物群落的潜在变化仍然知之甚少。我们比较了中国浙江两个城镇的非经营森林（NF）、减少经营森林（RF）和集约经营森林（IF）的根际土壤。随着管理强度的增加，土壤肥力和酶活性下降，细菌多样性和网络稳定性下降，真菌多样性有增加的趋势。细菌群落的聚集主要是确定性的，在集约化管理下变得更加明显，而真菌的聚集在很大程度上仍然是随机的。管理强度对微生物聚集没有直接调节作用；相反，土壤化学性质和酶活性介导了这些模式。一些微生物类群对管理强度响应强烈，并与群落组装过程显著相关。这些发现揭示了两种景观中管理强度和根际微生物模式之间的一致关联，尽管存在潜在的与地点相关的差异。降低管理强度有利于微生物网络的复杂性和稳定性，为杉木林业的可持续发展提供了基于微生物组学的途径。

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引用次数: 0

Contrasting strategies of two Camellia oleifera cultivars in shaping arbuscular mycorrhizal fungi communities under different phosphorus forms 两种油茶品种在不同磷形态下形成丛枝菌根真菌群落的策略比较

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2026-03-01 Epub Date: 2026-03-02 DOI: 10.1016/j.ejsobi.2026.103814

Yuxuan Huang , Xin You , Adam Frew , Fei Wu , Linping Zhang , Xinping Liu , Jiaoping Xing

Phosphorus (P) availability regulates the arbuscular mycorrhizal fungi (AMF) symbiosis, but the distinct effects of different P forms (soluble, insoluble, organic) and host plant genotypes on AMF communities remain underexplored. Using Camellia oleifera cultivars with contrasting P-use efficiencies (low-P-sensitive CL3 and low-P-tolerant CL40), we examined how P forms and cultivar identity shape AMF communities and their functional linkages to plant growth and soil nutrients. The results showed that soluble inorganic P (SP) maximized plant height and biomass but suppressed AMF diversity and simplified co-occurrence networks. In contrast, insoluble inorganic P (IP) enhanced AMF colonization rates and stabilized microbial interactions. The low-P-sensitive CL3 hosted a higher Chao1 index under P limitation, suggesting compensatory recruitment for P acquisition, while CL40 exhibited stronger soil P activation and maintained complex AMF networks. Glomus and Paraglomus were identified as core taxa in the rhizosphere AMF networks of C. oleifera. RDA and Mantel analyses showed that variation in plant growth and root traits was aligned with AMF characteristics, particularly colonization and core taxa (Glomus, Paraglomus), and strongly associated with soil nutrients, with SP treatment reducing mycorrhizal dependence and shifting associations toward Paraglomus. These insights inform targeted cultivar selection and phosphorus management to optimize C. oleifera production and maintain soil health.

磷(P)有效性调节丛枝菌根真菌（AMF）的共生关系，但不同磷形式（可溶性、不溶性、有机）和寄主植物基因型对AMF群落的显著影响尚不清楚。以不同磷利用效率（低磷敏感CL3和低磷耐受CL40）的油茶（Camellia oleifera）品种为研究对象，研究了磷形态和品种特性如何塑造AMF群落，以及它们与植物生长和土壤养分的功能联系。结果表明，可溶性无机磷（SP）可使植物株高和生物量最大化，但抑制AMF多样性，简化共生网络。相反，不溶性无机磷（IP）提高了AMF的定殖率，稳定了微生物间的相互作用。低磷敏感性CL3在磷限制下具有较高的Chao1指数，表明其对磷的补偿性招募，而CL40表现出更强的土壤磷激活，并维持复杂的AMF网络。球囊菌（Glomus）和Paraglomus是油桐根际AMF网络的核心类群。RDA和Mantel分析表明，植物生长和根系性状的变化与AMF特征一致，特别是定植和核心分类群（Glomus, Paraglomus），并与土壤养分密切相关，SP处理降低了菌根对Paraglomus的依赖，并将相关性转移到Paraglomus。这些见解为有针对性的品种选择和磷管理提供了信息，以优化油松产量并保持土壤健康。

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