Wentao Zhao , Fengli Su , Shulin Wang , Yujie Ma , Bingzhi Li , Xing Zhang
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引用次数: 0
Abstract
Exponential amplification reaction (EXPAR) is a synthetic biological circuit that can rapidly amplify short nucleic acid strands in an isothermal and exponential manner. EXPAR-enabled biosensors, developed through the integration of aptamers, nanomaterials, antibodies, and programmable nucleases, are widely applied in detecting both nucleic acid and non-nucleic acid targets critical in medical, environmental, and agricultural domains. This review summarizes recent advances in understanding and suppressing non-specific amplification in EXPAR, which is the most significant issue hampering the performance improvement of EXPAR-enabled biosensors. Subsequently, we introduce the application of EXPAR-enabled biosensors in detecting a broad range of analytes, with an emphasis on the strategies and their relevance to performance enhancement. Additionally, the trends in this area and the solutions associated with their challenges are discussed.
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TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.
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