MOF-mediated cascade reaction system for ultrasensitive detection of alkaline phosphatase activity and organophosphorus pesticides

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2024-10-15 DOI:10.1007/s00604-024-06757-8

Shengyuan Zhang, Ying Yang, Dezhi Yang, Yaling Yang

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Abstract

A metal–organic-framework (MOF) fluorescent sensor is reported based on NH₂-MIL-101(Fe) propelled pesticide and alkaline phosphatase (ALP) catalytic reaction. Different from previous reports, a cascade reaction system combined with MOF structural changes to generate fluorescence was employed. The rationale is that ALP can hydrolyze L-ascorbic acid 2-phosphate (AAP) into L-ascorbic acid (AA), which can reduce Fe³⁺ to decompose structurally NH₂-MIL-101(Fe), resulting in 2-aminoterephthalic acid (NH₂-BDC) with intense fluorescence. The fluorescence can be decreased to different degrees due to inhibition of organophosphate pesticides (OPPs) on the activity of ALP. By taking chlorpyrifos (CPY) as the model compound of an OPP pesticide and adding ALP and CPY into the NH₂-MIL-101(Fe) framework, the resulting cascade reaction fluorescence sensors exhibit a good sensitivity for CPY and ALP sensing. The working ranges are 0.02–2 μg/L and 0.2–20 mU/mL with detection limits (LOD) of 5.31 ng/L and 0.05 mU/mL, respectively. The proposed sensor has been actually applied to satisfactory detection of CPY and ALP in food and serum samples. This fluorescence-based assay may extend the application of MOF-based biosensors.

Graphical Abstract

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超灵敏检测碱性磷酸酶活性和有机磷农药的 MOF 介导级联反应系统

报告了一种基于 NH2-MIL-101(Fe)推进农药和碱性磷酸酶（ALP）催化反应的金属有机框架（MOF）荧光传感器。与以往报告不同的是，该研究采用了一个级联反应系统，结合 MOF 结构变化来产生荧光。其原理是 ALP 可将 L-抗坏血酸 2-磷酸酯（AAP）水解为 L-抗坏血酸（AA），而 L-抗坏血酸 2-磷酸酯（AAP）可还原 Fe3+，分解结构上的 NH2-MIL-101(Fe)，生成具有强烈荧光的 2-氨基对苯二甲酸（NH2-BDC）。由于有机磷农药（OPPs）对 ALP 活性的抑制，荧光会有不同程度的减弱。以毒死蜱（CPY）为有机磷农药模型化合物，在 NH2-MIL-101(Fe)框架中加入 ALP 和 CPY，得到的级联反应荧光传感器对 CPY 和 ALP 具有良好的灵敏度。其工作范围分别为 0.02-2 μg/L 和 0.2-20 mU/mL，检测限（LOD）分别为 5.31 ng/L 和 0.05 mU/mL。拟议的传感器已实际应用于食品和血清样品中 CPY 和 ALP 的检测，结果令人满意。这种基于荧光的检测方法可扩展基于 MOF 的生物传感器的应用范围。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.