Self-supported ultrathin PtRuMoCoNi high-entropy alloy nanowires (HEANWs) were synthesized by a one-pot co-reduction method, whose peroxidase (POD)-like activity and catalytic mechanism were elaborated in detail. As expected, the PtRuMoCoNi HEANWs showed excellent POD-like activity. It can quickly catalyze the oxidization of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue OXTMB through decomposition of H2O2 to superoxide radicals. Notably, isoniazid and hydrazine effectively scavenge the as-produced superoxide radicals and reduce the blue OXTMB, showing high reduction ability and antioxidant property. Thus, the PtRuMoCoNi HEANW-derived colorimetric method was developed for determination of isoniazid and hydrazine, which exhibited the linear ranges of 1.5 to 50 μM and 25 to 200 μM coupled with the lower detection limits of 2.3 and 12.6 μM for isoniazid and hydrazine, respectively. The excellent analytical performance mainly results from the synergistic catalytic effect of the multiple metals and distinctive ultra-thin nanowires. This work provides a simple and rapid colorimetric method for the determination of isoniazid and hydrazine in actual samples.
Graphical Abstract
{"title":"Electronic structure modulation of ultrathin PtRuMoCoNi high-entropy alloy nanowires for boosting peroxidase-like activity and sensitive colorimetric determination of isoniazid and hydrazine","authors":"Jia-Qi Li, Ai-Jun Wang, Pei Song, Jiu-Ju Feng, Qi Zhou, Tuck Yun Cheang","doi":"10.1007/s00604-024-06892-2","DOIUrl":"10.1007/s00604-024-06892-2","url":null,"abstract":"<div><p>Self-supported ultrathin PtRuMoCoNi high-entropy alloy nanowires (HEANWs) were synthesized by a one-pot co-reduction method, whose peroxidase (POD)-like activity and catalytic mechanism were elaborated in detail. As expected, the PtRuMoCoNi HEANWs showed excellent POD-like activity. It can quickly catalyze the oxidization of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue <sub>OX</sub>TMB through decomposition of H<sub>2</sub>O<sub>2</sub> to superoxide radicals. Notably, isoniazid and hydrazine effectively scavenge the as-produced superoxide radicals and reduce the blue <sub>OX</sub>TMB, showing high reduction ability and antioxidant property. Thus, the PtRuMoCoNi HEANW-derived colorimetric method was developed for determination of isoniazid and hydrazine, which exhibited the linear ranges of 1.5 to 50 μM and 25 to 200 μM coupled with the lower detection limits of 2.3 and 12.6 μM for isoniazid and hydrazine, respectively. The excellent analytical performance mainly results from the synergistic catalytic effect of the multiple metals and distinctive ultra-thin nanowires. This work provides a simple and rapid colorimetric method for the determination of isoniazid and hydrazine in actual samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-15DOI: 10.1007/s00604-024-06938-5
Xueqin Sun, Yan Fan, Wenyuan Zhang, Xiaogang Yang, Jiacheng Liu
A pentafluoropropionic acid-functionalized fluorescent metal-organic framework material (UiO-66-NH2-PFPA) is prepared by a simple post-synthetic modification (PSM) strategy for the sensitive and selective detection of dichloran (DCN). The results of fluorescence experiments demonstrate that the sensitivity of UiO-66-NH2-PFPA (limit of detection, LOD = 0.478 μM) to DCN is nearly 10.93 times higher than that of UiO-66-NH2 (LOD = 5.225 μM) and the material has good selectivity and anti-interference ability. After the addition of DCN, the blue fluorescence of UiO-66-NH2-PFPA is obviously quenched. Therefore, the possible quenching mechanism is further discussed in combination with relevant experiments and density functional theory calculations. Moreover, the sensor is applied to the detection of DCN in fruit samples with a satisfactory recovery of 101.1-107.9%, which implies that UiO-66-NH2-PFPA is expected to be a candidate material for the detection of DCN in food.
{"title":"A fluorescent sensor based on pentafluoropropanoic acid-functionalized UiO-66-NH<sub>2</sub> for enhanced selectivity and sensitivity of dicloran detection.","authors":"Xueqin Sun, Yan Fan, Wenyuan Zhang, Xiaogang Yang, Jiacheng Liu","doi":"10.1007/s00604-024-06938-5","DOIUrl":"https://doi.org/10.1007/s00604-024-06938-5","url":null,"abstract":"<p><p>A pentafluoropropionic acid-functionalized fluorescent metal-organic framework material (UiO-66-NH<sub>2</sub>-PFPA) is prepared by a simple post-synthetic modification (PSM) strategy for the sensitive and selective detection of dichloran (DCN). The results of fluorescence experiments demonstrate that the sensitivity of UiO-66-NH<sub>2</sub>-PFPA (limit of detection, LOD = 0.478 μM) to DCN is nearly 10.93 times higher than that of UiO-66-NH<sub>2</sub> (LOD = 5.225 μM) and the material has good selectivity and anti-interference ability. After the addition of DCN, the blue fluorescence of UiO-66-NH<sub>2</sub>-PFPA is obviously quenched. Therefore, the possible quenching mechanism is further discussed in combination with relevant experiments and density functional theory calculations. Moreover, the sensor is applied to the detection of DCN in fruit samples with a satisfactory recovery of 101.1-107.9%, which implies that UiO-66-NH<sub>2</sub>-PFPA is expected to be a candidate material for the detection of DCN in food.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":"87"},"PeriodicalIF":5.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-15DOI: 10.1007/s00604-024-06933-w
Shengnan Yin, Changlun Tong
A sensitized dual-response ratiometric fluorescent sensor integrated smartphone platform for accurate discrimination and detection of tetracycline (TC) homologues was fabricated based on N-CDs-Eu3+ complex. In the sensing system, N-CDs act as a sensitizer of Eu3+ and significantly enhance the fluorescence of TC-Eu3+ complex approximate 40-fold owing to the synergistic effect of antenna effect (AE) and fluorescence resonance energy transfer (FRET). A paper sensor integrated with a smartphone platform is further fabricated for on-site measurement of TC. The proposed sensing platform exhibits an obvious color change from blue to red with limit of detection (LOD) of 1.5 and 63.2 nM for spectrofluorimetry and paper sensor, respectively. In addition, to eliminate the interference from TC homologues, a simple and effective sensor array was constructed by regulating the pH of the system. The different fluorescence responses to four tetracycline homologues used widely (TC, OTC, CTC, and DOX) were examined and dealt with principal component analysis, and accurate differentiation of TC homologues was therefore achieved. This work provides an integrated method for identification and synchronous quantitative detection of TCs, and a potential application for visual and on-site detection of TCs in environmental monitoring and food safety.
{"title":"A sensitized dual-response ratiometric fluorescent sensor integrated smartphone platform for accurate discrimination and detection of tetracycline homologues based on N-CDs‒Eu<sup>3+</sup> complex.","authors":"Shengnan Yin, Changlun Tong","doi":"10.1007/s00604-024-06933-w","DOIUrl":"https://doi.org/10.1007/s00604-024-06933-w","url":null,"abstract":"<p><p>A sensitized dual-response ratiometric fluorescent sensor integrated smartphone platform for accurate discrimination and detection of tetracycline (TC) homologues was fabricated based on N-CDs-Eu<sup>3+</sup> complex. In the sensing system, N-CDs act as a sensitizer of Eu<sup>3+</sup> and significantly enhance the fluorescence of TC-Eu<sup>3+</sup> complex approximate 40-fold owing to the synergistic effect of antenna effect (AE) and fluorescence resonance energy transfer (FRET). A paper sensor integrated with a smartphone platform is further fabricated for on-site measurement of TC. The proposed sensing platform exhibits an obvious color change from blue to red with limit of detection (LOD) of 1.5 and 63.2 nM for spectrofluorimetry and paper sensor, respectively. In addition, to eliminate the interference from TC homologues, a simple and effective sensor array was constructed by regulating the pH of the system. The different fluorescence responses to four tetracycline homologues used widely (TC, OTC, CTC, and DOX) were examined and dealt with principal component analysis, and accurate differentiation of TC homologues was therefore achieved. This work provides an integrated method for identification and synchronous quantitative detection of TCs, and a potential application for visual and on-site detection of TCs in environmental monitoring and food safety.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":"86"},"PeriodicalIF":5.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A Cr-doped VO2 nanobelt (Cr/VO2) with remarkable peroxidase-like activity was synthesized and coupled with uricase to catalyze the cascade reaction for detection of uric acid. Notably, the affinity of Cr/VO2 for 3,3',5,5'-tetramethylbenzidine dihydrochloride hydrate (TMB) and hydrogen peroxide (H2O2) is tenfold and 20-fold higher, respectively, than that of horseradish peroxidase (HRP). The Cr/VO2 exhibits highly reactive and stable peroxidase activity at temperatures of 20-60 ℃. Thus, the reaction catalyzed by Cr/VO2 can be carried out at room temperature, greatly simplifying the testing process. This enzyme cascade nanozyme based colorimetric assay enables the selective and simple detection of uric acid within 30 min with a detection limit of only 0.34 μM, and has an observable potential in clinical applications.
Graphical abstract
{"title":"Enzyme cascade nanozyme based colorimetric sensor for detection of uric acid as a biomarker of hyperuricemia","authors":"Wenrui Ke, Guochen Kuang, Chuangqi Gu, Junhao Wang, Xiao Jiang, Rongjiao Zhu, Haiyuan Wang, Zhen Zhang","doi":"10.1007/s00604-024-06946-5","DOIUrl":"10.1007/s00604-024-06946-5","url":null,"abstract":"<div><p> A Cr-doped VO<sub>2</sub> nanobelt (Cr/VO<sub>2</sub>) with remarkable peroxidase-like activity was synthesized and coupled with uricase to catalyze the cascade reaction for detection of uric acid. Notably, the affinity of Cr/VO<sub>2</sub> for 3,3',5,5'-tetramethylbenzidine dihydrochloride hydrate (TMB) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is tenfold and 20-fold higher, respectively, than that of horseradish peroxidase (HRP). The Cr/VO<sub>2</sub> exhibits highly reactive and stable peroxidase activity at temperatures of 20-60 ℃. Thus, the reaction catalyzed by Cr/VO<sub>2</sub> can be carried out at room temperature, greatly simplifying the testing process. This enzyme cascade nanozyme based colorimetric assay enables the selective and simple detection of uric acid within 30 min with a detection limit of only 0.34 μM, and has an observable potential in clinical applications.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06946-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-15DOI: 10.1007/s00604-024-06921-0
Yajun Zhao, Ming Guan, Fang Mi, Yiyao Zhang, Pengfei Geng, Shan Zhang, Han Song, Guotong Chen
A AuNSs@PB@Ag-Apt surface-enhanced Raman scattering (SERS) probe has been developed by embedding Prussian blue (PB) between Au core and Ag shell. The PB SERS probe illustrates strong SERS activity in the Raman silent region of 2070 cm−1, and has a zero background signal, ensuring high sensitivity for the detection of Staphylococcus aureus (S.aureus). Moreover, the assemble SERS probe exhibits the catalytic function of peroxidase (POD), enabling the catalysis of 3,3′,5,5′-tetramethylbenzidine (TMB) for colorimetric detection. Additionally, we prepared lectin-functionalized magnetic nanoparticles (F-MNPs-ConA) that demonstrate superior adsorption capabilities towards S. aureus, achieving an impressive adsorption value of up to 98.62%. The F-MNPs-ConA/S. aureus/AuNSs@PB@Ag-Apt sandwich structure constructed by the above SERS probe and magnetic nanoparticles achieves highly sensitive detection of S. aureus. The detection range is 1 × 100 ~ 1 × 108 CFU·mL−1, and the detection limit (LOD) is 1 CFU·mL−1. Notably, this method can be integrated into smartphones, facilitating rapid and on-site detection of S. aureus. Compared with similar biosensors, this biosensor offers distinct advantages, including a wide detection range and a low detection limit. The reliability of the biosensor was confirmed by its application in milk, where the recovery ranged from 93.66 to 120.8%. The dual-mode SERS/colorimetric detection, non-interfering nature, magnetic enrichment, and reusability of the biosensor make it a promising tool for point-of-care testing (POCT).
Graphical Abstract
{"title":"A SERS/colorimetric biosensor based on AuNSs@Ag core–shell Prussian blue nanozyme for non-interference and rapid detection of Staphylococcus aureus in milk","authors":"Yajun Zhao, Ming Guan, Fang Mi, Yiyao Zhang, Pengfei Geng, Shan Zhang, Han Song, Guotong Chen","doi":"10.1007/s00604-024-06921-0","DOIUrl":"10.1007/s00604-024-06921-0","url":null,"abstract":"<div><p>A AuNSs@PB@Ag-Apt surface-enhanced Raman scattering (SERS) probe has been developed by embedding Prussian blue (PB) between Au core and Ag shell. The PB SERS probe illustrates strong SERS activity in the Raman silent region of 2070 cm<sup>−1</sup>, and has a zero background signal, ensuring high sensitivity for the detection of <i>Staphylococcus aureus </i>(<i>S.</i> <i>aureus</i>). Moreover, the assemble SERS probe exhibits the catalytic function of peroxidase (POD), enabling the catalysis of 3,3′,5,5′-tetramethylbenzidine (TMB) for colorimetric detection. Additionally, we prepared lectin-functionalized magnetic nanoparticles (F-MNPs-ConA) that demonstrate superior adsorption capabilities towards <i>S. aureus</i>, achieving an impressive adsorption value of up to 98.62%. The F-MNPs-ConA/<i>S. aureus</i>/AuNSs@PB@Ag-Apt sandwich structure constructed by the above SERS probe and magnetic nanoparticles achieves highly sensitive detection of <i>S. aureus</i>. The detection range is 1 × 10<sup>0</sup> ~ 1 × 10<sup>8</sup> CFU·mL<sup>−1</sup>, and the detection limit (LOD) is 1 CFU·mL<sup>−1</sup>. Notably, this method can be integrated into smartphones, facilitating rapid and on-site detection of <i>S. aureus</i>. Compared with similar biosensors, this biosensor offers distinct advantages, including a wide detection range and a low detection limit. The reliability of the biosensor was confirmed by its application in milk, where the recovery ranged from 93.66 to 120.8%. The dual-mode SERS/colorimetric detection, non-interfering nature, magnetic enrichment, and reusability of the biosensor make it a promising tool for point-of-care testing (POCT).</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06921-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A convenient method is proposed using a heat-treatable volatile template to prepare hierarchical porous biochar (HPB). Litsea cubeba leaves and ZIF-8 served as carbon source and volatile hard template, respectively. The good compatibility between ZIF-8 and biomass facilitated their uniform dispersion, and the thermal decomposition of ZIF-8 created abundant pores in the HPB. By using ZIF-8 with different sizes as “modulators”, the textural properties of HPB can be controlled. The HPB derived from 200 nm-sized ZIF-8 exhibited ultra-high specific surface area, rich macro-meso-micro porous structure, and excellent electrocatalytic activity, which makes HPB-200 an effective electrochemical sensing platform. The sensor demonstrated an ultralow detection limit (0.2 nM) of Ponceau 4R, showing significant application potential in the field of electroanalytical sensing.
Graphical Abstract
Schematic illustration of a convenient method using a heat-treatable volatile template to prepare hierarchical porous biochar (HPB)
{"title":"A novel hierarchical porous biochar based on ZIF-8 volatile hard template with high-efficiency electrochemical sensing performance for trace determination of Ponceau 4R","authors":"Qi Yu, Jing Zhou, Wenhai Wang, Rumin Fu, Wentao Yan, Hui Chen, Yansha Gao, Guorong Fan, Linyu Wang, Zongde Wang, Limin Lu","doi":"10.1007/s00604-025-06960-1","DOIUrl":"10.1007/s00604-025-06960-1","url":null,"abstract":"<div><p>A convenient method is proposed using a heat-treatable volatile template to prepare hierarchical porous biochar (HPB). <i>Litsea cubeba</i> leaves and ZIF-8 served as carbon source and volatile hard template, respectively. The good compatibility between ZIF-8 and biomass facilitated their uniform dispersion, and the thermal decomposition of ZIF-8 created abundant pores in the HPB. By using ZIF-8 with different sizes as “modulators”, the textural properties of HPB can be controlled. The HPB derived from 200 nm-sized ZIF-8 exhibited ultra-high specific surface area, rich macro-meso-micro porous structure, and excellent electrocatalytic activity, which makes HPB-200 an effective electrochemical sensing platform. The sensor demonstrated an ultralow detection limit (0.2 nM) of Ponceau 4R, showing significant application potential in the field of electroanalytical sensing.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div><div><p>Schematic illustration of a convenient method using a heat-treatable volatile template to prepare hierarchical porous biochar (HPB)</p></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-14DOI: 10.1007/s00604-025-06954-z
Xigui Liu, Danlian Huang, Cui Lai, Fei Wang
A simple and rapid colorimetric detection strategy, based on hydrogen bond identification of 6-thioguanine (6-TG) functionalized Au nanoparticles (AuNPs), is proposed for highly selective and sensitive determination of kanamycin (KA). In this strategy, the hydrogen bond interaction between 6-TG and kanamycin induces AuNPs to agglomerate, with a consequent color change of AuNPs from wine red to purple or even blue. The kanamycin concentrations can be quantified by employing UV–vis spectrophotometer. The results display that kanamycin concentrations (0.005 to 18 µM) are linearly related to A620/A520 (the absorbance ratio of 620 nm and 520 nm) with a LOD of 1.8 nM and a LOQ of 5.9 nM (S/N = 3). This strategy also reveals a high degree of selectivity among a series of common interfering species. Moreover, the strategy can be employed to detect trace amounts of kanamycin in real-life samples, and it shows satisfying results compared with high performance liquid chromatography. In general, this developed strategy is facile and inexpensive without the need for complex processing procedures and expensive instruments. In addition, this work may further exploit detection strategies for other organic contaminants, as well as make a strong contribution to the development of the colorimetric method.
Graphical Abstract
{"title":"Visual detection of kanamycin with functionalized Au nanoparticles","authors":"Xigui Liu, Danlian Huang, Cui Lai, Fei Wang","doi":"10.1007/s00604-025-06954-z","DOIUrl":"10.1007/s00604-025-06954-z","url":null,"abstract":"<div><p>A simple and rapid colorimetric detection strategy, based on hydrogen bond identification of 6-thioguanine (6-TG) functionalized Au nanoparticles (AuNPs), is proposed for highly selective and sensitive determination of kanamycin (KA). In this strategy, the hydrogen bond interaction between 6-TG and kanamycin induces AuNPs to agglomerate, with a consequent color change of AuNPs from wine red to purple or even blue. The kanamycin concentrations can be quantified by employing UV–vis spectrophotometer. The results display that kanamycin concentrations (0.005 to 18 µM) are linearly related to A<sub>620</sub>/A<sub>520</sub> (the absorbance ratio of 620 nm and 520 nm) with a LOD of 1.8 nM and a LOQ of 5.9 nM (S/N = 3). This strategy also reveals a high degree of selectivity among a series of common interfering species. Moreover, the strategy can be employed to detect trace amounts of kanamycin in real-life samples, and it shows satisfying results compared with high performance liquid chromatography. In general, this developed strategy is facile and inexpensive without the need for complex processing procedures and expensive instruments. In addition, this work may further exploit detection strategies for other organic contaminants, as well as make a strong contribution to the development of the colorimetric method.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-14DOI: 10.1007/s00604-024-06877-1
Niaz Jahed Tabrizi, Mohammad Amjadi, Tooba Hallaj
Fe, Ni, and Cu doped ceria nanoparticles (CeNPs) were prepared with a simple and one-pot hydrothermal synthesis method. We investigated the chemiluminescence (CL) interaction between these NPs and rhodamine B (Rh B) and found that the highest CL intensity was related to the Rh B- Cu doped CeNPs. We assigned that to the higher catalytic property of Cu doped NPs compared to the others. Cu doped CeNPs have been applied for the first time as a catalyst in chemiluminescence reactions. Rh B- Cu doped CeNPs reaction was introduced as a novel CL system, and its mechanism was studied. Considering the sensitivity, simplicity, portability, and rapidness of the CL methods, we applied the introduced reaction to the development of a CL sensor for nitrite monitoring. In the presence of nitrite, the CL intensity of the system decreased, and there was a linear relationship between the CL intensity and nitrite concentration in the range 0.5–100 µM. Based on this fact, a sensitive and selective CL sensor with the detection limit of 0.2 µM was established for nitrite detection in various food and water samples.
Graphical Abstract
{"title":"Cu doped ceria nanoparticles- rhodamine B as a novel chemiluminescence system and its application for nitrite detection in water and food samples","authors":"Niaz Jahed Tabrizi, Mohammad Amjadi, Tooba Hallaj","doi":"10.1007/s00604-024-06877-1","DOIUrl":"10.1007/s00604-024-06877-1","url":null,"abstract":"<div><p>Fe, Ni, and Cu doped ceria nanoparticles (CeNPs) were prepared with a simple and one-pot hydrothermal synthesis method. We investigated the chemiluminescence (CL) interaction between these NPs and rhodamine B (Rh B) and found that the highest CL intensity was related to the Rh B- Cu doped CeNPs. We assigned that to the higher catalytic property of Cu doped NPs compared to the others. Cu doped CeNPs have been applied for the first time as a catalyst in chemiluminescence reactions. Rh B- Cu doped CeNPs reaction was introduced as a novel CL system, and its mechanism was studied. Considering the sensitivity, simplicity, portability, and rapidness of the CL methods, we applied the introduced reaction to the development of a CL sensor for nitrite monitoring. In the presence of nitrite, the CL intensity of the system decreased, and there was a linear relationship between the CL intensity and nitrite concentration in the range 0.5–100 µM. Based on this fact, a sensitive and selective CL sensor with the detection limit of 0.2 µM was established for nitrite detection in various food and water samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A spherical nucleic acid (SNA, AuNPs-aptamer) into CRISPR/Cas12a system combined with poly T-template copper nanoparticles as fluorescence reporter was fabricated to establish an amplification-free sensitive method for Staphylococcus aureus (S. aureus) detection. This method, named PTCas12a, utilizes the concept that the bifunction of SNA recognizes the S. aureus and triggers the Cas12a cleavage activity. Then, the Cas12a enzyme cleaves the Poly T40 to generate a signal change in Poly T-Cu fluorescence, indicating the presence or absence of the target bacteria. The PTCas12a platform demonstrated a detection limit as low as 3.0 CFU/mL (3 N/S) in a wide response range of 1.0 × 101–1.0 × 106 CFU/mL for S. aureus detection, which holds significant potential in ensuring food safety and preventing the spread of diseases.
Graphical abstract
{"title":"Amplification-free sensitive detection of Staphylococcus aureus by spherical nucleic acid triggered CRISPR/Cas12a and Poly T-Cu reporter","authors":"Xiaoyu Zhang, Ruimeng Sun, Haoran Zheng, Yanfei Qi","doi":"10.1007/s00604-024-06931-y","DOIUrl":"10.1007/s00604-024-06931-y","url":null,"abstract":"<div><p>A spherical nucleic acid (SNA, AuNPs-aptamer) into CRISPR/Cas12a system combined with poly T-template copper nanoparticles as fluorescence reporter was fabricated to establish an amplification-free sensitive method for <i>Staphylococcus aureus</i> (<i>S. aureus</i>) detection. This method, named PTCas12a, utilizes the concept that the bifunction of SNA recognizes the <i>S. aureus</i> and triggers the Cas12a cleavage activity. Then, the Cas12a enzyme cleaves the Poly T40 to generate a signal change in Poly T-Cu fluorescence, indicating the presence or absence of the target bacteria. The PTCas12a platform demonstrated a detection limit as low as 3.0 CFU/mL (3 N/S) in a wide response range of 1.0 × 10<sup>1</sup>–1.0 × 10<sup>6</sup> CFU/mL for <i>S. aureus</i> detection, which holds significant potential in ensuring food safety and preventing the spread of diseases.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A novel electrochemical aptasensor based on bimetallic zirconium and copper oxides embedded within mesoporous carbon (denoted as ZrO2CuOx@mC) was constructed to detect miRNA. The porous ZrO2CuOx@mC was created through the pyrolysis of bimetallic zirconium/copper-based metal–organic framework (ZrCu-MOF). The substantial surface area and high porosity of ZrO2CuOx@mC nanocomposite along with its robust affinity toward aptamer strands, facilitated the effective anchoring of aptamer strands on the ZrO2CuOx@mC-modified electrode surface. This, coupled with the remarkable electrochemical performance arising from the presence of metal oxides and mesoporous carbon, resulted in the exceptional sensitivity of the ZrO2CuOx@mC-based aptasensor for the detection of miR-21. The prepared aptasensor not only demonstrated a broad detection range from 10 zM to 100 pM, featuring an exceptionally low detection limit of 0.52 zM, but also exhibited notable selectivity against interferences. Moreover, it displayed good stability, reproducibility, and acceptable applicability for miR-21 detection in human serum. The fabricated aptasensor offers a promising platform for ultrasensitive miR-21 detection, with potential applications in accurate and early diagnosis of diseases related to miRNA.
Graphical Abstract
{"title":"Electrochemical aptasensor based on zirconium/copper oxides embedded in mesoporous carbon derived from bimetallic metal–organic framework for ultrasensitive detection of miR-21","authors":"Leila Gorgani, Maedeh Mohammadi, Ghasem Najafpour Darzi, Jahan Bakhsh Raoof","doi":"10.1007/s00604-024-06925-w","DOIUrl":"10.1007/s00604-024-06925-w","url":null,"abstract":"<div><p>A novel electrochemical aptasensor based on bimetallic zirconium and copper oxides embedded within mesoporous carbon (denoted as ZrO<sub>2</sub>CuO<sub>x</sub>@mC) was constructed to detect miRNA. The porous ZrO<sub>2</sub>CuO<sub>x</sub>@mC was created through the pyrolysis of bimetallic zirconium/copper-based metal–organic framework (ZrCu-MOF). The substantial surface area and high porosity of ZrO<sub>2</sub>CuO<sub>x</sub>@mC nanocomposite along with its robust affinity toward aptamer strands, facilitated the effective anchoring of aptamer strands on the ZrO<sub>2</sub>CuO<sub>x</sub>@mC-modified electrode surface. This, coupled with the remarkable electrochemical performance arising from the presence of metal oxides and mesoporous carbon, resulted in the exceptional sensitivity of the ZrO<sub>2</sub>CuO<sub>x</sub>@mC-based aptasensor for the detection of miR-21. The prepared aptasensor not only demonstrated a broad detection range from 10 zM to 100 pM, featuring an exceptionally low detection limit of 0.52 zM, but also exhibited notable selectivity against interferences. Moreover, it displayed good stability, reproducibility, and acceptable applicability for miR-21 detection in human serum. The fabricated aptasensor offers a promising platform for ultrasensitive miR-21 detection, with potential applications in accurate and early diagnosis of diseases related to miRNA.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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