Pub Date : 2025-02-18DOI: 10.1007/s00604-024-06911-2
Yifan Yan, Pandeng Miao, Shuaijing Du, Yingxiang Du
A novel nanomaterial synthesized by chiral molecularly imprinted polymers (CMIPs) and achiral metal–organic frameworks (MOFs) was designed as stationary phase to prepare L-TRP@MIP(APTES-TEOS)@UiO-66-NH2@capillary for tryptophan enantioseparation in open tubular capillary electrochromatography. Compared with the capillary column coated only with CMIPs or achiral MOFs, this column remarkably improved the enantioseparation ability of tryptophan (resolution, 0.92/0 → 3.68). The chromatographic conditions (buffer pH, applied voltage, organic additive content) were optimized. Additionally, through static adsorption experiments, a conclusion was reached that the materials of stationary phase had stronger adsorption capacity for L-TRP than that for D-TRP, which revealed chiral separation mechanism of CEC system. This study opens up creative ideas for coating the novel nanomaterial in CEC system, which has good application prospects in the field of chiral separation.
Graphical Abstract
{"title":"Enantioseparation system based on a novel nanomaterial synthesized from chiral molecularly imprinted polymers and achiral metal–organic frameworks by capillary electrochromatography","authors":"Yifan Yan, Pandeng Miao, Shuaijing Du, Yingxiang Du","doi":"10.1007/s00604-024-06911-2","DOIUrl":"10.1007/s00604-024-06911-2","url":null,"abstract":"<div><p>A novel nanomaterial synthesized by chiral molecularly imprinted polymers (CMIPs) and achiral metal–organic frameworks (MOFs) was designed as stationary phase to prepare L-TRP@MIP(APTES-TEOS)@UiO-66-NH<sub>2</sub>@capillary for tryptophan enantioseparation in open tubular capillary electrochromatography. Compared with the capillary column coated only with CMIPs or achiral MOFs, this column remarkably improved the enantioseparation ability of tryptophan (resolution, 0.92/0 → 3.68). The chromatographic conditions (buffer pH, applied voltage, organic additive content) were optimized. Additionally, through static adsorption experiments, a conclusion was reached that the materials of stationary phase had stronger adsorption capacity for L-TRP than that for D-TRP, which revealed chiral separation mechanism of CEC system. This study opens up creative ideas for coating the novel nanomaterial in CEC system, which has good application prospects in the field of chiral separation.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-18DOI: 10.1007/s00604-025-07020-4
Tatiana G. Choleva, Vasiliki I. Karagianni, Dimosthenis L. Giokas
A paper-based UV dosimeter/indicator based on the solid-state photochemical reduction of gold-cationic surfactant complexes is described. Upon exposure to UV light, the photoreductive elimination of chloride ions from the CTA-AuCl4− complex leads to the reduction of Au ions and the formation of small Au nuclei. After hydration, the Au nuclei are washed away by capillary flow and produce gold nanoparticles by coalescence and aggregation, resulting in a blue-purple coloration on the paper surface. The reaction is initiated by UV light of variable wavelength (from UVA at 254 nm tο UVC at 365 nm) and over a wide range of UV doses (up to 3000 mJ/cm2 for UVB at 312 nm and UVC at 365 nm). This versatility allows for its application in monitoring light exposure in germicidal sterilization, UV phototherapy, and as a personal solar UV indicator. The user-friendly paper facilitates both the fabrication and use of the devices, which can be activated on demand by simply wetting the paper surface.
Graphical abstract
{"title":"Hydrochromic paper-based dosimeter for monitoring UV light exposure based on the photochemical formation of gold nanoparticles","authors":"Tatiana G. Choleva, Vasiliki I. Karagianni, Dimosthenis L. Giokas","doi":"10.1007/s00604-025-07020-4","DOIUrl":"10.1007/s00604-025-07020-4","url":null,"abstract":"<div><p>A paper-based UV dosimeter/indicator based on the solid-state photochemical reduction of gold-cationic surfactant complexes is described. Upon exposure to UV light, the photoreductive elimination of chloride ions from the CTA-AuCl<sub>4</sub><sup>−</sup> complex leads to the reduction of Au ions and the formation of small Au nuclei. After hydration, the Au nuclei are washed away by capillary flow and produce gold nanoparticles by coalescence and aggregation, resulting in a blue-purple coloration on the paper surface. The reaction is initiated by UV light of variable wavelength (from UVA at 254 nm tο UVC at 365 nm) and over a wide range of UV doses (up to 3000 mJ/cm<sup>2</sup> for UVB at 312 nm and UVC at 365 nm). This versatility allows for its application in monitoring light exposure in germicidal sterilization, UV phototherapy, and as a personal solar UV indicator. The user-friendly paper facilitates both the fabrication and use of the devices, which can be activated on demand by simply wetting the paper surface.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07020-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-18DOI: 10.1007/s00604-025-07031-1
Lingling Wang, Shengjun Bu, Heyuan Meng, Xiaoying Zhang, Hongyu Zhou, Chuanna Xu, Zhuo Hao, Yuchun Su, Jiayu Wan
A lateral flow biosensor was developed based on newly established primer extension nucleic acid isothermal amplification (PEIA) and M13mp18 single-stranded DNA (ssDNA) to visually identify the H1N1 virus. The PEIA process was primer based on a template to continuously extend multiple repeat sequences, using DNA polymerase, to form long ssDNAs. M13mp18 ssDNA was then coupled to multiple long ssDNAs from PEIA and functionalized in a signal tag DNA nanostructure (STDN) for the lateral flow biosensor. In the presence of target H1N1-RNA, the sandwich structure capture probe/H1N1-RNA/STDN is fabricated caught in the lateral flow biosensor test zone, and appears red. Our biosensor successfully identified H1N1 virus RNA over a broad range of concentrations (10–106 pM) with a minimum visual detection limit of 10 pM. In conclusion, our biosensor may be applied to point-of-care diagnostics in low-resource environments with non-specialist staff.
Graphical Abstract
{"title":"Lateral flow biosensor development for the visual identification of H1N1 virus based on primer extension nucleic acid isothermal amplification and M13mp18 single-stranded DNA","authors":"Lingling Wang, Shengjun Bu, Heyuan Meng, Xiaoying Zhang, Hongyu Zhou, Chuanna Xu, Zhuo Hao, Yuchun Su, Jiayu Wan","doi":"10.1007/s00604-025-07031-1","DOIUrl":"10.1007/s00604-025-07031-1","url":null,"abstract":"<div><p> A lateral flow biosensor was developed based on newly established primer extension nucleic acid isothermal amplification (PEIA) and M13mp18 single-stranded DNA (ssDNA) to visually identify the H1N1 virus. The PEIA process was primer based on a template to continuously extend multiple repeat sequences, using DNA polymerase, to form long ssDNAs. M13mp18 ssDNA was then coupled to multiple long ssDNAs from PEIA and functionalized in a signal tag DNA nanostructure (STDN) for the lateral flow biosensor. In the presence of target H1N1-RNA, the sandwich structure capture probe/H1N1-RNA/STDN is fabricated caught in the lateral flow biosensor test zone, and appears red. Our biosensor successfully identified H1N1 virus RNA over a broad range of concentrations (10–10<sup>6</sup> pM) with a minimum visual detection limit of 10 pM. In conclusion, our biosensor may be applied to point-of-care diagnostics in low-resource environments with non-specialist staff.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-18DOI: 10.1007/s00604-025-07038-8
Yutong Wang, Xiao Liang, Yaling Yang
Se-WCDs-Au-Janus Ag NPs with weak oxidase activity were synthesized using Se-W carbon dots (Se-WCDs) as stabilizers and reducing agents. These Janus nanoparticles (Janus NPs) effectively catalyze the reduction of mercury ions (Hg2+) to metallic mercury (Hg0), generating Au–Ag-Hg mercury alloys (Au–Ag@HgNPs). The aggregated Au–Ag@HgNPs on the nanoparticles improve the SERS activity of Se-WCDs-Au-Janus Ag NPs through a synergistic effect of electromagnetic enhancement and chemical enhancement. Interestingly, adding FB1 turns off the Raman signal by inhibiting the aggregation of Au–Ag@HgNPs. Based on these findings, a SERS “on–off” sensor utilizing the Se-WCDs-Au-Janus Ag NPs as the probe was successfully developed to determine fumonisin B1 (FB1) and Hg2+ in grains. The detection limits were as low as 0.005 μg·L−1 for Hg2+ and 0.006 μg·L−1 for FB1.
Graphical abstract
{"title":"A SERS “on–off” sensor for Hg2+ and fumonisin B1 determination in grains based on Au-Janus Ag NPs modified by carbon dots","authors":"Yutong Wang, Xiao Liang, Yaling Yang","doi":"10.1007/s00604-025-07038-8","DOIUrl":"10.1007/s00604-025-07038-8","url":null,"abstract":"<div><p>Se-WCDs-Au-Janus Ag NPs with weak oxidase activity were synthesized using Se-W carbon dots (Se-WCDs) as stabilizers and reducing agents. These Janus nanoparticles (Janus NPs) effectively catalyze the reduction of mercury ions (Hg<sup>2+</sup>) to metallic mercury (Hg<sup>0</sup>), generating Au–Ag-Hg mercury alloys (Au–Ag@HgNPs). The aggregated Au–Ag@HgNPs on the nanoparticles improve the SERS activity of Se-WCDs-Au-Janus Ag NPs through a synergistic effect of electromagnetic enhancement and chemical enhancement. Interestingly, adding FB1 turns off the Raman signal by inhibiting the aggregation of Au–Ag@HgNPs. Based on these findings, a SERS “on–off” sensor utilizing the Se-WCDs-Au-Janus Ag NPs as the probe was successfully developed to determine fumonisin B1 (FB1) and Hg<sup>2+</sup> in grains. The detection limits were as low as 0.005 μg·L<sup>−1</sup> for Hg<sup>2+</sup> and 0.006 μg·L<sup>−1</sup> for FB1.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-17DOI: 10.1007/s00604-024-06906-z
Ziwei Zou, Yi Chen, Rengang Sun, Boya Shi, Li Jiang, Feixiang Huang
A surface plasmon resonance imaging (SPRi) biosensor is presented which facilitates rapid and sensitive detection of the ovarian cancer biomarker carbohydrate antigens 125 (CA125). The CA125 protein was specifically captured and directly recognized by polyadenylic (polyA)-modified DNA aptamer on the surface of a Au chip. The biosensor demonstrated a linear range of direct detection of CA125 based on SPRi from 20 nM to 0.5 nM, with a detection limit of 0.47 nM. Furthermore, Fe3O4 nanoparticles were synthesized in situ on the carboxyl-functionalized two-dimensional (2D) material Ti3C2 to obtain MXC/Fe3O4 nanocomposites. The large surface area of Ti3C2 provides ample binding sites for the in situ generation of Fe3O4 and this facilitates subsequent immobilization of DNA aptamers. The amino-modified DNA aptamer interacted with carboxylate sites activated on the surface of Ti3C2, and the aptamer was immobilized on the MXC/Fe3O4 nanocomposite surface. Due to the magnetic properties of Fe3O4 nanoparticles within the composite material, CA125 protein in serum can be effectively captured and separated under an external magnetic field. The captured protein forms a sandwich amplification structure with the DNA adapter on the surface of the Au chip, thereby enhancing the detection signal of CA125. The biosensor demonstrates a linear detection range from 0.5 pM to 1000 pM, with a detection limit as low as 81 fM. The unique separation capability of the composite nanomaterials enables the biosensor to detect CA125 protein as low as 1 pM in complex liquid matrices such as serum, achieving exceptional selectivity, accuracy, and sensitivity in detecting the ovarian cancer marker. This capability significantly supports the early diagnosis of ovarian cancer.
Graphical abstract
{"title":"Ti3C2/Fe3O4 - based surface plasmon resonance imaging biosensor for efficient separation and sensitive detection of CA125 in serum","authors":"Ziwei Zou, Yi Chen, Rengang Sun, Boya Shi, Li Jiang, Feixiang Huang","doi":"10.1007/s00604-024-06906-z","DOIUrl":"10.1007/s00604-024-06906-z","url":null,"abstract":"<div><p> A surface plasmon resonance imaging (SPRi) biosensor is presented which facilitates rapid and sensitive detection of the ovarian cancer biomarker carbohydrate antigens 125 (CA125). The CA125 protein was specifically captured and directly recognized by polyadenylic (polyA)-modified DNA aptamer on the surface of a Au chip. The biosensor demonstrated a linear range of direct detection of CA125 based on SPRi from 20 nM to 0.5 nM, with a detection limit of 0.47 nM. Furthermore, Fe<sub>3</sub>O<sub>4</sub> nanoparticles were synthesized in situ on the carboxyl-functionalized two-dimensional (2D) material Ti<sub>3</sub>C<sub>2</sub> to obtain MXC/Fe<sub>3</sub>O<sub>4</sub> nanocomposites. The large surface area of Ti<sub>3</sub>C<sub>2</sub> provides ample binding sites for the in situ generation of Fe<sub>3</sub>O<sub>4</sub> and this facilitates subsequent immobilization of DNA aptamers. The amino-modified DNA aptamer interacted with carboxylate sites activated on the surface of Ti<sub>3</sub>C<sub>2</sub>, and the aptamer was immobilized on the MXC/Fe<sub>3</sub>O<sub>4</sub> nanocomposite surface. Due to the magnetic properties of Fe<sub>3</sub>O<sub>4</sub> nanoparticles within the composite material, CA125 protein in serum can be effectively captured and separated under an external magnetic field. The captured protein forms a sandwich amplification structure with the DNA adapter on the surface of the Au chip, thereby enhancing the detection signal of CA125. The biosensor demonstrates a linear detection range from 0.5 pM to 1000 pM, with a detection limit as low as 81 fM. The unique separation capability of the composite nanomaterials enables the biosensor to detect CA125 protein as low as 1 pM in complex liquid matrices such as serum, achieving exceptional selectivity, accuracy, and sensitivity in detecting the ovarian cancer marker. This capability significantly supports the early diagnosis of ovarian cancer.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-16DOI: 10.1007/s00604-024-06927-8
Zelong Fan, Lingling Yuan, Tingcheng Xia, Chengxian Zhao, Wenjuan Guo, Ahmed A. Ibrahim, Sajid Ali Ansari, Ahmad Umar
The development of a highly sensitive electrochemical aptasensor is reported that combines the unique properties of graphene, metallic oxides, and conducting polymers to overcome the limitations of sensitivity and specificity required for early diagnosis of Alzheimer's desease (AD). The sensor design utilizes a ternary composite of polypyrrole (PPy), reduced graphene oxide (rGO), and Fe₂O₃ nanoparticles, which synergistically enhance signal amplification, conductivity, and biocompatibility. The aptasensor leverages aptamers as biorecognition elements for the specific detection of Aβ1-40 oligomers. Electrochemical techniques, including cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS), were employed to characterize and evaluate the sensor's performance. The fabricated aptasensor demonstrated an extraordinary detection limit of 40 fM and a broad linear detection range from 0.1 pM to 200 nM, with exceptional sensitivity and selectivity. Furthermore, the sensor exhibited excellent reproducibility and stability, essential for real-world clinical applications. Real-sample testing using artificial serum further validated the aptasensor's reliability, showcasing its potential for early diagnosis and monitoring of AD. The robust platform and modular design of the sensor also pave the way for its adaptation to detect other target biomolecules by simply modifying the aptamer. This work represents a significant advancement in biosensor technology, offering a versatile, highly sensitive tool for biomedical diagnostics and personalized medicine.
Graphical Abstract
{"title":"Enhanced Alzheimer's biomarker detection using a ternary composite electrochemical aptasensor","authors":"Zelong Fan, Lingling Yuan, Tingcheng Xia, Chengxian Zhao, Wenjuan Guo, Ahmed A. Ibrahim, Sajid Ali Ansari, Ahmad Umar","doi":"10.1007/s00604-024-06927-8","DOIUrl":"10.1007/s00604-024-06927-8","url":null,"abstract":"<div><p>The development of a highly sensitive electrochemical aptasensor is reported that combines the unique properties of graphene, metallic oxides, and conducting polymers to overcome the limitations of sensitivity and specificity required for early diagnosis of Alzheimer's desease (AD). The sensor design utilizes a ternary composite of polypyrrole (PPy), reduced graphene oxide (rGO), and Fe₂O₃ nanoparticles, which synergistically enhance signal amplification, conductivity, and biocompatibility. The aptasensor leverages aptamers as biorecognition elements for the specific detection of Aβ1-40 oligomers. Electrochemical techniques, including cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS), were employed to characterize and evaluate the sensor's performance. The fabricated aptasensor demonstrated an extraordinary detection limit of 40 fM and a broad linear detection range from 0.1 pM to 200 nM, with exceptional sensitivity and selectivity. Furthermore, the sensor exhibited excellent reproducibility and stability, essential for real-world clinical applications. Real-sample testing using artificial serum further validated the aptasensor's reliability, showcasing its potential for early diagnosis and monitoring of AD. The robust platform and modular design of the sensor also pave the way for its adaptation to detect other target biomolecules by simply modifying the aptamer. This work represents a significant advancement in biosensor technology, offering a versatile, highly sensitive tool for biomedical diagnostics and personalized medicine.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A label-free photoelectrochemical (PEC) biosensing strategy was constructed based on a Cu2MoS4@Ti3C2Tx MXene heterojunction for the sensitive detection of tetracycline (TC) in milk. The Cu2MoS4@Ti3C2Tx MXene heterojunction substantially facilitates the separation of photogenerated electron–hole pairs, a key element in enhancing the photocurrent response. Cu2MoS4, renowned for its visible-light absorption and photocatalytic properties, is combined synergistically with Ti3C2Tx MXene, contributing a large surface area and outstanding electrical conductivity. The heterojunction not only elevates photocatalytic efficiency but also provides abundant π–π stacking sites on the surface of Ti3C2Tx MXene. These sites enable the direct immobilization of the TC aptamer without chemical modification, thereby minimizing marker interference and improving the detection accuracy. Furthermore, the heterojunction structure broadens the light-absorption spectrum and enhances charge-transfer efficiency, resulting in a higher photocurrent response. The proposed PEC aptasensor demonstrates a favorable linear response to TC within the concentration range 5 to 300 nM, with a detection limit of 1.24 nM. These results are comparable to those achieved by high-performance liquid chromatography. This study is expected to pioneer a novel approach for the design of a label-free PEC aptasensor applicable to the bioanalysis of food samples.
Graphical abstract
{"title":"Highly sensitive label-free photoelectrochemical aptasensor based on Cu2MoS4@Ti3C2Tx MXene heterojunction for tetracycline detection in milk","authors":"Yuying Xu, Lijuan Chen, Xiaohui Guo, Jian Zhang, Xiaobing Guo, Ziqian He, Jiani Zhang, Dongxue Han","doi":"10.1007/s00604-025-07016-0","DOIUrl":"10.1007/s00604-025-07016-0","url":null,"abstract":"<div><p> A label-free photoelectrochemical (PEC) biosensing strategy was constructed based on a Cu<sub>2</sub>MoS<sub>4</sub>@Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene heterojunction for the sensitive detection of tetracycline (TC) in milk. The Cu<sub>2</sub>MoS<sub>4</sub>@Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene heterojunction substantially facilitates the separation of photogenerated electron–hole pairs, a key element in enhancing the photocurrent response. Cu<sub>2</sub>MoS<sub>4</sub>, renowned for its visible-light absorption and photocatalytic properties, is combined synergistically with Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene, contributing a large surface area and outstanding electrical conductivity. The heterojunction not only elevates photocatalytic efficiency but also provides abundant π–π stacking sites on the surface of Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene. These sites enable the direct immobilization of the TC aptamer without chemical modification, thereby minimizing marker interference and improving the detection accuracy. Furthermore, the heterojunction structure broadens the light-absorption spectrum and enhances charge-transfer efficiency, resulting in a higher photocurrent response. The proposed PEC aptasensor demonstrates a favorable linear response to TC within the concentration range 5 to 300 nM, with a detection limit of 1.24 nM. These results are comparable to those achieved by high-performance liquid chromatography. This study is expected to pioneer a novel approach for the design of a label-free PEC aptasensor applicable to the bioanalysis of food samples.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A highly selective and sensitive silicon nanoparticles (Si NPs) biosensor was developed for dopamine (DA) detection utilizing synchronous fluorescence spectroscopy (SFS). Si NPs were synthesized via a one-step hydrothermal method, utilizing 3-aminopropyltriethoxysilane (APTES) as the silane precursor and polyethyleneimine (PEI) as the reducing agent. The Si NPs exhibited a spherical morphology, with an average diameter of 3.7 nm. Additionally, the Si NPs demonstrated remarkable fluorescent properties. When integrated into the DA detection process utilizing SFS, the synchronous fluorescence spectrum of the DA system exclusively exhibits a peak at 530 nm, whereas systems involving epinephrine and norepinephrine both display peaks at 484 nm and 530 nm. Consequently, this detection system exhibits a high capacity for effectively differentiating between DA and other catecholamines (epinephrine and norepinephrine), ensuring a high degree of specificity in the detection process. Additionally, this combined approach also presents a strong linear relationship between the enhancement of synchronous fluorescence intensity (F530/F484) and DA concentrations within the concentration ranges 1–10 μM and 10–50 μM, respectively. The limit of detection (LOD) was 0.22 μM (3σ/k). Finally, the biosensor was successfully employed for the detection of DA in the spiked serum samples. This work introduced a novel approach for DA detection and provided a valuable technical platform for rapid monitoring of DA levels in biological samples.
Graphical abstract
{"title":"A synchronous fluorescence biosensor mediated by hydrogen-bonding interaction for highly selective detection of dopamine","authors":"Lingling Li, Xinru Zhang, Xianglei Chang, Xianhua Meng, Weifeng Wang, Ying Zhang, Aimei Yang, Junli Yang","doi":"10.1007/s00604-025-06993-6","DOIUrl":"10.1007/s00604-025-06993-6","url":null,"abstract":"<div><p> A highly selective and sensitive silicon nanoparticles (Si NPs) biosensor was developed for dopamine (DA) detection utilizing synchronous fluorescence spectroscopy (SFS). Si NPs were synthesized via a one-step hydrothermal method, utilizing 3-aminopropyltriethoxysilane (APTES) as the silane precursor and polyethyleneimine (PEI) as the reducing agent. The Si NPs exhibited a spherical morphology, with an average diameter of 3.7 nm. Additionally, the Si NPs demonstrated remarkable fluorescent properties. When integrated into the DA detection process utilizing SFS, the synchronous fluorescence spectrum of the DA system exclusively exhibits a peak at 530 nm, whereas systems involving epinephrine and norepinephrine both display peaks at 484 nm and 530 nm. Consequently, this detection system exhibits a high capacity for effectively differentiating between DA and other catecholamines (epinephrine and norepinephrine), ensuring a high degree of specificity in the detection process. Additionally, this combined approach also presents a strong linear relationship between the enhancement of synchronous fluorescence intensity (F<sub>530</sub>/F<sub>484</sub>) and DA concentrations within the concentration ranges 1–10 <i>μ</i>M and 10–50 <i>μ</i>M, respectively. The limit of detection (LOD) was 0.22 <i>μ</i>M (3<i>σ</i>/<i>k</i>). Finally, the biosensor was successfully employed for the detection of DA in the spiked serum samples. This work introduced a novel approach for DA detection and provided a valuable technical platform for rapid monitoring of DA levels in biological samples.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MiRNA-208a is a molecular marker for the early stage of acute myocardial infarction (AMI), with satisfactory specificity and a relatively short time window, making it highly suitable for the early diagnosis of AMI. In this study, a CuNCs-cDNA-MoS2 fluorescent probe is designed and a Förster resonance energy transfer (FRET) enzyme-free fluorescent biosensor is established for “on-off-on” detection of miRNA-208a. The results showed that the detection limit for miRNA-208a was 900 pM, with a linear range of 1 to 10 nM. Interestingly, after the addition of RNase inhibitor, the detection time and limit for serum miRNA-208a were reduced to 5 min and 380 pM, respectively. It also has a wide linear range (1-20 nM), with a recovery of 98.13 to 101.20%. Compared to traditional methods, this strategy is simple, rapid, efficient, and cost-effective. In summary, the FRET-based fluorescent sensor provides a high-performance method for detecting miRNA-208a.
Graphical Abstract
{"title":"Luminescence “on-off-on” sensing of acute myocardial infarction biomarker miRNA-208a based on copper nanoclusters-MoS2 FRET system","authors":"Suzhen Zhang, Danling Mei, Hongming Fang, Quanwei Li, Xiumei Zhou, Xueqin Zhao, Zhencang Zheng, Biao Huang","doi":"10.1007/s00604-024-06940-x","DOIUrl":"10.1007/s00604-024-06940-x","url":null,"abstract":"<div><p>MiRNA-208a is a molecular marker for the early stage of acute myocardial infarction (AMI), with satisfactory specificity and a relatively short time window, making it highly suitable for the early diagnosis of AMI. In this study, a CuNCs-cDNA-MoS<sub>2</sub> fluorescent probe is designed and a Förster resonance energy transfer (FRET) enzyme-free fluorescent biosensor is established for “on-off-on” detection of miRNA-208a. The results showed that the detection limit for miRNA-208a was 900 pM, with a linear range of 1 to 10 nM. Interestingly, after the addition of RNase inhibitor, the detection time and limit for serum miRNA-208a were reduced to 5 min and 380 pM, respectively. It also has a wide linear range (1-20 nM), with a recovery of 98.13 to 101.20%. Compared to traditional methods, this strategy is simple, rapid, efficient, and cost-effective. In summary, the FRET-based fluorescent sensor provides a high-performance method for detecting miRNA-208a.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-14DOI: 10.1007/s00604-025-07015-1
Zhencui Wang, Jie Du, Xianman Zhang, Zhizhong Guo, Zhenyu Zhang, Wei Wen
Three-dimensional TiO2 hierarchical nanowire arrays were synthesized featuring nanocavities via a hydrogen peroxide–assisted wet chemical reaction, followed by a simple liquid-phase deposition process. We subsequently decorated the TiO2 arrays with dopamine, leveraging its enediol ligand for chelation. Reduced nicotinamide adenine dinucleotide (NADH), a cofactor for over 300 dehydrogenases, serves as the targeted analyte to assess the PEC sensing performance of the sensor. The developed sensor exhibited a linear concentration range for NADH detection of 0.05 to 50 µM, achieving a high sensitivity of 0.29 μA∙μM−1∙cm−2 and a low detection limit of 0.03 μM (signal-to-noise ratio of 3). Notably, PEC performance of the resulting sensor surpassed that of commercial TiO2 (P25)-based sensors. The enhanced sensing capabilities can be attributed to several factors: the TiO2 hierarchical nanowire arrays with nanocavities provide a larger surface area, abundant active sites, and shorter molecular diffusion lengths for surface adsorption and reaction, thereby reducing response times and improving sensitivity. Additionally, the coupling of TiO2 with dopamine enhances visible light absorption and optimizes charge transfer dynamics. These findings open a new avenue for designing high-performance three-dimensional nanoarchitectures for PEC sensing applications.
Graphical Abstract
{"title":"Three-dimensional titania arrays coupled with dopamine for visible-light-induced photoelectrochemical sensing","authors":"Zhencui Wang, Jie Du, Xianman Zhang, Zhizhong Guo, Zhenyu Zhang, Wei Wen","doi":"10.1007/s00604-025-07015-1","DOIUrl":"10.1007/s00604-025-07015-1","url":null,"abstract":"<div><p> Three-dimensional TiO<sub>2</sub> hierarchical nanowire arrays were synthesized featuring nanocavities via a hydrogen peroxide–assisted wet chemical reaction, followed by a simple liquid-phase deposition process. We subsequently decorated the TiO<sub>2</sub> arrays with dopamine, leveraging its enediol ligand for chelation. Reduced nicotinamide adenine dinucleotide (NADH), a cofactor for over 300 dehydrogenases, serves as the targeted analyte to assess the PEC sensing performance of the sensor. The developed sensor exhibited a linear concentration range for NADH detection of 0.05 to 50 µM, achieving a high sensitivity of 0.29 μA∙μM<sup>−1</sup>∙cm<sup>−2</sup> and a low detection limit of 0.03 μM (signal-to-noise ratio of 3). Notably, PEC performance of the resulting sensor surpassed that of commercial TiO<sub>2</sub> (P25)-based sensors. The enhanced sensing capabilities can be attributed to several factors: the TiO<sub>2</sub> hierarchical nanowire arrays with nanocavities provide a larger surface area, abundant active sites, and shorter molecular diffusion lengths for surface adsorption and reaction, thereby reducing response times and improving sensitivity. Additionally, the coupling of TiO<sub>2</sub> with dopamine enhances visible light absorption and optimizes charge transfer dynamics. These findings open a new avenue for designing high-performance three-dimensional nanoarchitectures for PEC sensing applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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