Pub Date : 2025-04-23DOI: 10.1007/s00604-025-07176-z
Yalda Haghighi Shishavan, Mohammad Amjadi
{"title":"Correction: Highly sensitive chemiluminometric and colorimetric probes for acetamiprid assay based on Cu2SnS3 quantum dots with peroxide-mimicking activity","authors":"Yalda Haghighi Shishavan, Mohammad Amjadi","doi":"10.1007/s00604-025-07176-z","DOIUrl":"10.1007/s00604-025-07176-z","url":null,"abstract":"","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-22DOI: 10.1007/s00604-025-07143-8
Yaru Liu, Yang Li, Hao Gong, Yao Liu, Yijie Wang, Cuiping Ma, Yuxi Wei, Chao Shi
An ultra-fast, easy-to-use and non-amplification electrochemical detection platform was constructed for microRNA (miRNA) detection. A set of label-free hairpin probes and capture probes were introduced to form a ternary complex, which could enhance the selectivity and stability of miRNA detection due to the ability of reducing non-specific bind with non-target and enhancing accessibility of target to probes. Moreover, the capture probe immobilization and hybridization process were accelerated by the external electric field, shortening the detection time from 2 h to 5 min. The platform showed a detection limit of 1.28 fM under ideal experimental control conditions and had ability to identify 1- or 2-nucleotide (nt) difference. In addition, the designed sensor achieved the sensitive determination of miRNA-21 in serum samples. The excellent anti-interference capability of this detection method indicated its potential for clinical application. Its simplicity and high specificity made this method a promising tool for detecting different miRNA to assist the diagnosis of diverse cancers.
Graphical Abstract
{"title":"A rapid, specific, and simple-to-use biosensor for amplification-free determination of microRNA based on electrical potential-assisted and ternary hybridization","authors":"Yaru Liu, Yang Li, Hao Gong, Yao Liu, Yijie Wang, Cuiping Ma, Yuxi Wei, Chao Shi","doi":"10.1007/s00604-025-07143-8","DOIUrl":"10.1007/s00604-025-07143-8","url":null,"abstract":"<div><p> An ultra-fast, easy-to-use and non-amplification electrochemical detection platform was constructed for microRNA (miRNA) detection. A set of label-free hairpin probes and capture probes were introduced to form a ternary complex, which could enhance the selectivity and stability of miRNA detection due to the ability of reducing non-specific bind with non-target and enhancing accessibility of target to probes. Moreover, the capture probe immobilization and hybridization process were accelerated by the external electric field, shortening the detection time from 2 h to 5 min. The platform showed a detection limit of 1.28 fM under ideal experimental control conditions and had ability to identify 1- or 2-nucleotide (nt) difference. In addition, the designed sensor achieved the sensitive determination of miRNA-21 in serum samples. The excellent anti-interference capability of this detection method indicated its potential for clinical application. Its simplicity and high specificity made this method a promising tool for detecting different miRNA to assist the diagnosis of diverse cancers.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-22DOI: 10.1007/s00604-025-07150-9
Siyu Wu, Hongyun Ma, Lin Song, Wen Zhong, Yingying Gu, Yuqing Miao, Yarui An
Carbohydrate antigen 19–9 (CA19 - 9) can be used as a biomarker for pancreatic cancer. Measuring the concentration of CA19 - 9 in serum is essential for screening pancreatic cancer patients. In this paper, a dual-electric signal outputs biosensor based on ping pong chrysanthemum-like Bi-BiOI and ternary core–shell structured Pd@AuPt was constructed for ultra-sensitive detection of tumor marker CA19 - 9 using differential pulse voltammetry (DPV) and chronoamperometry (i-t). Ping pong Chrysanthemum-like Bi-BIOI was prepared via one-pot hydrothermal method. To realize the covalent bonding of bismuth-based materials with MWCNT, bismuth-based materials were functionalized by amino groups. MWCNT-NH2-Bi-BIOI with large specific surface area and remarkable electrical conductivity was used as the sensing platform. Ternary core–shell structured Pd@AuPt with peroxide-like activity and enhanced biocompatibility immobilized massive antibodies through covalent Au–N and Pt–N bonds, thus broadening the linear range of the immunosensor. Based on the above materials, a dual-electric signal outputs biosensor was constructed for detecting CA19 - 9. Under optimal conditions, the detection range of DPV and i-t is 0.001–150 U/mL, the detection limit of DPV is 0.0003 U/mL, and that of i-t is 0.00024 U/mL. In addition, the dual-electric signal outputs immunoassay excels in anti-interference, splendid reproducibility and high recovery in actual sample detection, indicating that the immunosensor is a promising approach to be applied to the detection of CA19 - 9 in clinical diagnosis.
Graphical abstract
{"title":"Ping pong chrysanthemum-like Bi-BiOI and ternary core–shell structured Pd@AuPt based dual-electric signal outputs biosensor for accurate detection of CA19 - 9","authors":"Siyu Wu, Hongyun Ma, Lin Song, Wen Zhong, Yingying Gu, Yuqing Miao, Yarui An","doi":"10.1007/s00604-025-07150-9","DOIUrl":"10.1007/s00604-025-07150-9","url":null,"abstract":"<div><p>Carbohydrate antigen 19–9 (CA19 - 9) can be used as a biomarker for pancreatic cancer. Measuring the concentration of CA19 - 9 in serum is essential for screening pancreatic cancer patients. In this paper, a dual-electric signal outputs biosensor based on ping pong chrysanthemum-like Bi-BiOI and ternary core–shell structured Pd@AuPt was constructed for ultra-sensitive detection of tumor marker CA19 - 9 using differential pulse voltammetry (DPV) and chronoamperometry (i-t). Ping pong Chrysanthemum-like Bi-BIOI was prepared via one-pot hydrothermal method. To realize the covalent bonding of bismuth-based materials with MWCNT, bismuth-based materials were functionalized by amino groups. MWCNT-NH<sub>2</sub>-Bi-BIOI with large specific surface area and remarkable electrical conductivity was used as the sensing platform. Ternary core–shell structured Pd@AuPt with peroxide-like activity and enhanced biocompatibility immobilized massive antibodies through covalent Au–N and Pt–N bonds, thus broadening the linear range of the immunosensor. Based on the above materials, a dual-electric signal outputs biosensor was constructed for detecting CA19 - 9. Under optimal conditions, the detection range of DPV and i-t is 0.001–150 U/mL, the detection limit of DPV is 0.0003 U/mL, and that of i-t is 0.00024 U/mL. In addition, the dual-electric signal outputs immunoassay excels in anti-interference, splendid reproducibility and high recovery in actual sample detection, indicating that the immunosensor is a promising approach to be applied to the detection of CA19 - 9 in clinical diagnosis.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-22DOI: 10.1007/s00604-025-07175-0
Yi-Xian Liu, Bao-Zhu Jia, Xu-Chao Zhang, Fei Hou, Jun-Peng Liu, Wei-Yun Guo, Yuan Meng, Zhen-Lin Xu, Lin Luo
Chronic dietary intake of 17β-estradiol (E2) poses significant health risks, necessitating rapid and accurate analytical techniques for effective surveillance. This study presents a magnetic particle (MP)-assisted non-competitive chemiluminescent immunoassay (NCLIA) designed for E2 quantification in milk. The NCLIA employs anti-E2 monoclonal antibody (mAb)-coated MPs to capture E2 target molecules, followed by the recognition of acridinium ester (AE)-conjugated anti-E2@mAb immunocomplex antibody. Magnetic separation facilitates efficient isolation of immunocomplexes before automated luminescence measurement. This NCLIA demonstrated superior performance, without the need for complex pretreatment of milk samples, significantly simplifying the analysis process. The detection limit was as low as 1.57 pg/mL, and total detection time was 8 min. Additionally, NCLIA’s results were highly consistent with those obtained by liquid chromatography-tandem mass spectrometry (LC–MS/MS), validating their reliability in analyzing real samples. Therefore, this study presents an efficient and reliable tool for the rapid screening and detection of 17β-estradiol in milk, which holds significant value for food safety monitoring.
Graphical abstract
{"title":"Development of an ultrasensitive magnetic bead-based non-competitive chemiluminescent immunoassay for 17β-estradiol in milk","authors":"Yi-Xian Liu, Bao-Zhu Jia, Xu-Chao Zhang, Fei Hou, Jun-Peng Liu, Wei-Yun Guo, Yuan Meng, Zhen-Lin Xu, Lin Luo","doi":"10.1007/s00604-025-07175-0","DOIUrl":"10.1007/s00604-025-07175-0","url":null,"abstract":"<div><p>Chronic dietary intake of 17β-estradiol (E<sub>2</sub>) poses significant health risks, necessitating rapid and accurate analytical techniques for effective surveillance. This study presents a magnetic particle (MP)-assisted non-competitive chemiluminescent immunoassay (NCLIA) designed for E<sub>2</sub> quantification in milk. The NCLIA employs anti-E<sub>2</sub> monoclonal antibody (mAb)-coated MPs to capture E<sub>2</sub> target molecules, followed by the recognition of acridinium ester (AE)-conjugated anti-E<sub>2</sub>@mAb immunocomplex antibody. Magnetic separation facilitates efficient isolation of immunocomplexes before automated luminescence measurement. This NCLIA demonstrated superior performance, without the need for complex pretreatment of milk samples, significantly simplifying the analysis process. The detection limit was as low as 1.57 pg/mL, and total detection time was 8 min. Additionally, NCLIA’s results were highly consistent with those obtained by liquid chromatography-tandem mass spectrometry (LC–MS/MS), validating their reliability in analyzing real samples. Therefore, this study presents an efficient and reliable tool for the rapid screening and detection of 17β-estradiol in milk, which holds significant value for food safety monitoring.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-21DOI: 10.1007/s00604-025-07147-4
Sara Escudero-Cernuda, Noemi Eiro, María Fraile, Francisco J. Vizoso, Belén Fernández-Colomer, María Luisa Fernández-Sánchez
Exosomes are a subpopulation of nanosized extracellular vesicles (EVs), formed by a lipid bilayer and naturally secreted by cells. They transport RNA, microRNAs, bioactive proteins, and lipids and play an important role in intercellular communication. Exosomes are a promising alternative cell-free therapy in regenerative medicine, immunotherapy, and drug delivery. The implementation of new exosomes treatments requires knowledge of its concentration, purity, and full characterization. However, comparing different studies is highly challenging due to the lack of validated methodologies for isolation and determination, as well as a lack of well-characterized exosomes reference standards. In this work, human uterine cervical mesenchymal stem cell (hUCESC) EVs have been isolated by ultracentrifugation and characterized, discussing the current limitations of characterization methods. First, total protein assays are heavily influenced by free-protein and lipid contaminations which confirm the need of employing several methods for vesicular protein determination. This purity variation seems heavily influenced by the vesicles origin as more complex mediums originate more matrix interferences. Size exclusion high performance liquid chromatography has been demonstrated as a new methodology for purity assessment of hUCESC-EVs and commercial EVs (adipose stem cells and human serum). The results found low purity in the commercial exosomes highlighting that protein and lipid purity must be included in the commercial EVs. Finally, the combination of this chromatography method with total protein assays proved that particle concentration could be estimated using vesicular protein concentration.
Graphical abstract
{"title":"Limitations and challenges in the characterization of extracellular vesicles from stem cells and serum","authors":"Sara Escudero-Cernuda, Noemi Eiro, María Fraile, Francisco J. Vizoso, Belén Fernández-Colomer, María Luisa Fernández-Sánchez","doi":"10.1007/s00604-025-07147-4","DOIUrl":"10.1007/s00604-025-07147-4","url":null,"abstract":"<div><p>Exosomes are a subpopulation of nanosized extracellular vesicles (EVs), formed by a lipid bilayer and naturally secreted by cells. They transport RNA, microRNAs, bioactive proteins, and lipids and play an important role in intercellular communication. Exosomes are a promising alternative cell-free therapy in regenerative medicine, immunotherapy, and drug delivery. The implementation of new exosomes treatments requires knowledge of its concentration, purity, and full characterization. However, comparing different studies is highly challenging due to the lack of validated methodologies for isolation and determination, as well as a lack of well-characterized exosomes reference standards. In this work, human uterine cervical mesenchymal stem cell (hUCESC) EVs have been isolated by ultracentrifugation and characterized, discussing the current limitations of characterization methods. First, total protein assays are heavily influenced by free-protein and lipid contaminations which confirm the need of employing several methods for vesicular protein determination. This purity variation seems heavily influenced by the vesicles origin as more complex mediums originate more matrix interferences. Size exclusion high performance liquid chromatography has been demonstrated as a new methodology for purity assessment of hUCESC-EVs and commercial EVs (adipose stem cells and human serum). The results found low purity in the commercial exosomes highlighting that protein and lipid purity must be included in the commercial EVs. Finally, the combination of this chromatography method with total protein assays proved that particle concentration could be estimated using vesicular protein concentration.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07147-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-21DOI: 10.1007/s00604-025-07151-8
Ruxia Zhang, Qi Wang, Zhefeng Fan
Malachite green (MG), one of the most toxic veterinary drug residues, is of great significance to establish a rapid, accurate, and highly selective on-site quantitative detection method for food safety. In this study, a new strategy for the detection of MG in aquatic products is developed, which is based on orange fluorescent carbon dots (O-CDs) as nanoswitches for rapid and reversible detection of MG content. The overlap between the strong absorption of MG at 615 nm and the fluorescence emission spectra of O-CDs at 564 nm provides effective support for the internal filter effect (IFE) leading to fluorescence quenching of the system. Under the attack of negative hydrogen ions in NaBH4, MG is effectively reduced to weakly absorb leucomalachite green (LMG), blocking the IFE process and leading to the recovery of system fluorescence, thereby achieving reversible detection of MG within 30 s with up to 9 cycles. In solution, the strategy demonstrates a wide linear range of 1–120 μM for MG with a detection limit of 0.37 μM. In addition, a smartphone-assisted O-CDs agar slice sensing platform has been developed, which can accurately quantify and visually determine MG. Furthermore, it is also utilized for fluorescence imaging of exogenous MG in zebrafish. In summary, the establishment of sensing strategy provides a solution for constructing qualitative and visual semi quantitative sensors on site, which has potential application value in food safety assessment.
Graphical abstract
{"title":"Reversible and ultrafast fluorescent nanoswitch for on-site visual determination of malachite green and in vivo zebrafish imaging","authors":"Ruxia Zhang, Qi Wang, Zhefeng Fan","doi":"10.1007/s00604-025-07151-8","DOIUrl":"10.1007/s00604-025-07151-8","url":null,"abstract":"<div><p>Malachite green (MG), one of the most toxic veterinary drug residues, is of great significance to establish a rapid, accurate, and highly selective on-site quantitative detection method for food safety. In this study, a new strategy for the detection of MG in aquatic products is developed, which is based on orange fluorescent carbon dots (O-CDs) as nanoswitches for rapid and reversible detection of MG content. The overlap between the strong absorption of MG at 615 nm and the fluorescence emission spectra of O-CDs at 564 nm provides effective support for the internal filter effect (IFE) leading to fluorescence quenching of the system. Under the attack of negative hydrogen ions in NaBH<sub>4</sub>, MG is effectively reduced to weakly absorb leucomalachite green (LMG), blocking the IFE process and leading to the recovery of system fluorescence, thereby achieving reversible detection of MG within 30 s with up to 9 cycles. In solution, the strategy demonstrates a wide linear range of 1–120 μM for MG with a detection limit of 0.37 μM. In addition, a smartphone-assisted O-CDs agar slice sensing platform has been developed, which can accurately quantify and visually determine MG. Furthermore, it is also utilized for fluorescence imaging of exogenous MG in zebrafish. In summary, the establishment of sensing strategy provides a solution for constructing qualitative and visual semi quantitative sensors on site, which has potential application value in food safety assessment.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-21DOI: 10.1007/s00604-025-07170-5
Na Li, Yiqun Guo, Mengmeng Wu, Aiying Wang, Yuna Guo, Yuancheng Li
A highly specific and ultrasensitive electrochemical immunosensor for malachite green (MG) detection in complex food matrices is presented. The sensor was constructed through a stepwise assembly process incorporating gold nanoparticles, antibodies, and metal–organic framework (MOF)-derived Pd/CuO@NiO nanocomposites. Its structure and electrochemical performance were thoroughly validated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Key detection parameters, including pH and nanocomposite concentration, were systematically optimized to maximize sensor performance. The sensor demonstrated a broad linear detection range (10−4 to 100 ng/mL) and an ultralow detection limit (42 pg/L). Specificity tests confirmed the immunosensor’s ability to selectively detect MG without interference from structurally similar compounds such as crystal violet, nitrofuran drugs, and chloramphenicol. Its practical applicability was verified using pretreated freshwater fish samples spiked with MG, yielding recoveries of 95%–105%. Furthermore, the results showed strong agreement with those of the conventional enzyme-linked immunosorbent assays (ELISA) method, with minimal difference ratios observed. These findings establish the immunosensor as a reliable, accurate, and rapid tool for detecting MG in food safety applications. Looking ahead, the platform’s modular design and versatility provide opportunities to extend its application to other harmful contaminants in food and environmental monitoring, contributing to broader advancements in public health and safety.
Graphical abstract
{"title":"High-performance electrochemical immunosensor for ultrasensitive detection of malachite green in food matrices using MOF-derived nanocomposites","authors":"Na Li, Yiqun Guo, Mengmeng Wu, Aiying Wang, Yuna Guo, Yuancheng Li","doi":"10.1007/s00604-025-07170-5","DOIUrl":"10.1007/s00604-025-07170-5","url":null,"abstract":"<div><p> A highly specific and ultrasensitive electrochemical immunosensor for malachite green (MG) detection in complex food matrices is presented. The sensor was constructed through a stepwise assembly process incorporating gold nanoparticles, antibodies, and metal–organic framework (MOF)-derived Pd/CuO@NiO nanocomposites. Its structure and electrochemical performance were thoroughly validated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Key detection parameters, including pH and nanocomposite concentration, were systematically optimized to maximize sensor performance. The sensor demonstrated a broad linear detection range (10<sup>−4</sup> to 100 ng/mL) and an ultralow detection limit (42 pg/L). Specificity tests confirmed the immunosensor’s ability to selectively detect MG without interference from structurally similar compounds such as crystal violet, nitrofuran drugs, and chloramphenicol. Its practical applicability was verified using pretreated freshwater fish samples spiked with MG, yielding recoveries of 95%–105%. Furthermore, the results showed strong agreement with those of the conventional enzyme-linked immunosorbent assays (ELISA) method, with minimal difference ratios observed. These findings establish the immunosensor as a reliable, accurate, and rapid tool for detecting MG in food safety applications. Looking ahead, the platform’s modular design and versatility provide opportunities to extend its application to other harmful contaminants in food and environmental monitoring, contributing to broader advancements in public health and safety.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-21DOI: 10.1007/s00604-025-07166-1
Nisha Jain, Navneet Kaur
A sulfonamide derivative synthesized from 1,8-naphthalimide-based amine and 4-methoxy-benzenesulfonyl chloride has been enwrapped into the micelles of a neutral surfactant TX-100 to form highly emissive nanomicelles MXL@TX- 100. The proposed sensor offered highly sensitive turn-off detection of Allura red (linear range = 0.66–224 µM) with the limit of detection (LOD) of 0.2 µM (R2 = 0.999) due to fluorescence resonance energy transfer from MXL@TX- 100 to Allura red. To the best of our knowledge, no organic compound-based sensor has been reported for the determination of Allura red so far. Additionally, nanomicelles MXL@TX- 100 were utilized for the sensing of picric acid (LOD = 0.92 µM, R2 = 0.998) in the linear range 3.03–928 µM. The binding constant between MXL@TX- 100-Allura red and MXL@TX- 100-picric were calculated to be 1.45 × 105 M−1 and 1.97 × 104 M−1, respectively. Moreover, nanomicelles MXL@TX- 100 conveniently detected Allura red in real samples, viz. candy, soft drink, gulkand, and pharmaceutical syrup.
Graphical Abstract
{"title":"Construction of highly emissive Schiff base containing TX- 100 nanomicelles for monitoring the levels of devil in disguise Allura red and picric acid in real samples","authors":"Nisha Jain, Navneet Kaur","doi":"10.1007/s00604-025-07166-1","DOIUrl":"10.1007/s00604-025-07166-1","url":null,"abstract":"<div><p>A sulfonamide derivative synthesized from 1,8-naphthalimide-based amine and 4-methoxy-benzenesulfonyl chloride has been enwrapped into the micelles of a neutral surfactant TX-100 to form highly emissive nanomicelles <b>MXL@TX- 100</b>. The proposed sensor offered highly sensitive turn-off detection of Allura red (linear range = 0.66–224 µM) with the limit of detection (LOD) of 0.2 µM (<i>R</i><sup>2</sup> = 0.999) due to fluorescence resonance energy transfer from <b>MXL@TX- 100</b> to Allura red. To the best of our knowledge, no organic compound-based sensor has been reported for the determination of Allura red so far. Additionally, nanomicelles <b>MXL@TX- 100</b> were utilized for the sensing of picric acid (LOD = 0.92 µM, <i>R</i><sup>2</sup> = 0.998) in the linear range 3.03–928 µM. The binding constant between <b>MXL@TX- 100-</b>Allura red and <b>MXL@TX- 100-</b>picric were calculated to be 1.45 × 10<sup>5</sup> M<sup>−1</sup> and 1.97 × 10<sup>4</sup> M<sup>−1</sup>, respectively. Moreover, nanomicelles <b>MXL@TX- 100</b> conveniently detected Allura red in real samples, viz. candy, soft drink, gulkand, and pharmaceutical syrup.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A novel, low-toxicity Ru-Ni-BPY metal–organic framework (MOF) nanomaterial was developed and a simple and sensitive dual-mode "on–off" ferric ions (Fe3+) sensing platform based on this material constructed. The dual-mode detection method enabled cross-validation of the electrochemiluminescence (ECL) and fluorescent (FL) signals, enhancing accuracy and effectively minimizing errors. In linear ranges of 0.005–10 μM and 0.078–10 μM, Fe3+ can dramatically quench the ECL and FL signals of the Ru-Ni-BPY MOF, with quenching rates of 91.42% and 91.31%, respectively, and detection limits of 0.1 nM and 9.3 nM, respectively. This ECL method not only achieved efficient detection of Fe3+ in rat brain microdialysis fluid but also successfully enabled real-time FL detection of Fe3+ in nude mice. Therefore, this platform has great potential for applications in environmental protection, life sciences, and early disease diagnosis.
Graphical abstract
{"title":"A ruthenium-nickel metal–organic framework incorporating 2,2'-bipyridine- 5,5'-dicarboxylic acid for dual-mode electrochemiluminescence-fluorescence detection of ferric ions","authors":"Jing Zhang, Zhaojiang Yin, Wei Xiong, QiQi Fan, Fengyao Yu, Fusheng Liao, Hao Fan, Fei Qu, Qiangqiang Yu","doi":"10.1007/s00604-025-07168-z","DOIUrl":"10.1007/s00604-025-07168-z","url":null,"abstract":"<div><p>A novel, low-toxicity Ru-Ni-BPY metal–organic framework (MOF) nanomaterial was developed and a simple and sensitive dual-mode \"on–off\" ferric ions (Fe<sup>3+</sup>) sensing platform based on this material constructed. The dual-mode detection method enabled cross-validation of the electrochemiluminescence (ECL) and fluorescent (FL) signals, enhancing accuracy and effectively minimizing errors. In linear ranges of 0.005–10 μM and 0.078–10 μM, Fe<sup>3+</sup> can dramatically quench the ECL and FL signals of the Ru-Ni-BPY MOF, with quenching rates of 91.42% and 91.31%, respectively, and detection limits of 0.1 nM and 9.3 nM, respectively. This ECL method not only achieved efficient detection of Fe<sup>3+</sup> in rat brain microdialysis fluid but also successfully enabled real-time FL detection of Fe<sup>3+</sup> in nude mice. Therefore, this platform has great potential for applications in environmental protection, life sciences, and early disease diagnosis.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-17DOI: 10.1007/s00604-025-07157-2
Ling Shi, Anping Li, Yuying Xu, Hongping Yang, Guangming Yang
A novel thieno[3,2-b]thiophene (TT) was utilized for the first time to electrochemically fabricate an ionic liquid (IL)-supported polythieno[3,2-b]thiophene (PTT) coating. This innovative coating serves as a new headspace solid-phase microextraction (HS-SPME) material for the extraction of five phenolic compounds, which were subsequently determined via gas chromatography-mass spectrometry (GC–MS). The coating was characterized using Fourier-transform infrared spectroscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. Experimental results confirmed that the ILs were successfully embedded within the polymer matrix, resulting in a well-defined three-dimensional mesoporous architecture. This unique architecture not only exhibited high thermal stability but also maintained a consistent extraction performance when used for the solid-phase microextraction of phenolic compounds. To optimize the extraction and detection performance, various experimental parameters were investigated, including the coating type, pH, salt concentration, extraction time, extraction temperature, stirring rate, desorption time, and temperature. Under the optimal conditions identified, the method exhibited low limits of detection ranging from 0.001 to 0.007 μg mL−1 and wide linearity in the concentration range 0.030 to 10.000 μg mL−1. Noteworthily, the proposed method was successfully applied to the determination of five phenols in actual water samples, achieving satisfactory recoveries between 90.5% and 110.5%.
Graphical Abstract
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