Different patterns of bacterioplankton in response to inorganic and organic phosphorus inputs in freshwater lakes - a microcosmic study

Abstract

Phosphorus (P) is a limiting factor in fresh waters and is also the main cause of water eutrophication and deterioration, However, the practical effect of elevated P level on bacterioplankton is less evaluated. In this study, we investigated the bacterioplankton in a 96 hours microcosm experiment with P additions in two forms (organic/inorganic P, OP/IP) and three levels (final conc., 0.040, 0.065 and 0.125 g/L), aiming to find out the response pattern of bacterioplankton in coping with the increasing P levels. Results showed a more dramatic change of water properties and bacterioplankton between P forms (OP and IP) than among the addition levels, and a more remarkable effect of OP addition than the IP. Both OP and IP treatments significantly decreased the water pH, dissolved oxygen (DO), Electrical Conductivity (EC), Nitrate Nitrogen (NO₃^--N) and Total Organic Carbon (TOC), and reduced the α-diversity of bacterioplankton and relative abundance of Cyanobacteria, but increased the abundance of Proteobacteria. The IP addition decreased Actinobacteria abundance (especially for HgcI) and showed higher denitrification potentials, while the OP addition depressed the Bateroidota and exhibited lowed methylotrophic functions, but such trends decreased with increasing addition concentrations. The network analysis showed that both IP and OP additions increased the proportion of positively correlated edges and reduced the network complexity and stability, but the OP network was more stable than the IP network. The study clarifies the response pattern of bacterioplankton to the P input with different forms and levels, and deepens our understanding of the eutrophication process, which provides a scientific basis for the management and control of freshwater lakes facing eutrophication.