Periostin+ myeloid cells improved long bone regeneration in a mechanosensitive manner

IF 14.3 1区 医学 Q1 CELL & TISSUE ENGINEERING Bone Research Pub Date : 2024-10-15 DOI:10.1038/s41413-024-00361-5
Ziyan Wang, Minmin Lin, Yonghao Pan, Yang Liu, Chengyu Yang, Jianqun Wu, Yan Wang, Bingtong Yan, Jingjing Zhou, Rouxi Chen, Chao Liu
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Abstract

Myeloid cells are pivotal in the inflammatory and remodeling phases of fracture repair. Here, we investigate the effect of periostin expressed by myeloid cells on bone regeneration in a monocortical tibial defect (MTD) model. In this study, we show that periostin is expressed by periosteal myeloid cells, primarily the M2 macrophages during bone regeneration. Knockout of periostin in myeloid cells reduces cortical bone thickness, disrupts trabecular bone connectivity, impairs repair impairment, and hinders M2 macrophage polarization. Mechanical stimulation is a regulator of periostin in macrophages. By activating transforming growth factor-β (TGF-β), it increases periostin expression in macrophages and induces M2 polarization. This mechanosensitive effect also reverses the delayed bone repair induced by periostin deficiency in myeloid cells by strengthening the angiogenesis-osteogenesis coupling. In addition, transplantation of mechanically conditioned macrophages into the periosteum over a bone defect results in substantially enhanced repair, confirming the critical role of macrophage-secreted periostin in bone repair. In summary, our findings suggest that mechanical stimulation regulates periostin expression and promotes M2 macrophage polarization, highlighting the potential of mechanically conditioned macrophages as a therapeutic strategy for enhancing bone repair.

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髓磷脂+髓系细胞以机械敏感的方式改善长骨再生
髓系细胞在骨折修复的炎症和重塑阶段起着关键作用。在此,我们研究了在单皮质胫骨缺损(MTD)模型中,髓系细胞表达的骨粘连蛋白对骨再生的影响。在这项研究中,我们发现在骨再生过程中,骨膜髓系细胞,主要是 M2 巨噬细胞表达了骨粘连蛋白。敲除髓质细胞中的骨粘连蛋白可减少皮质骨厚度,破坏骨小梁的连接,损害修复功能,并阻碍 M2 巨噬细胞的极化。机械刺激是巨噬细胞中骨膜增生蛋白的调节器。通过激活转化生长因子-β(TGF-β),它能增加巨噬细胞中的骨膜增生蛋白表达并诱导 M2 极化。这种机械敏感效应还能通过加强血管生成-骨生成耦合,逆转髓系细胞因缺乏骨膜增生蛋白而导致的骨修复延迟。此外,将经机械调节的巨噬细胞移植到骨缺损处的骨膜中,可大大增强修复效果,这证实了巨噬细胞分泌的骨膜素在骨修复中的关键作用。总之,我们的研究结果表明,机械刺激可调控骨粘连蛋白的表达并促进 M2 巨噬细胞的极化,这凸显了机械调理巨噬细胞作为加强骨修复治疗策略的潜力。
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来源期刊
Bone Research
Bone Research CELL & TISSUE ENGINEERING-
CiteScore
20.00
自引率
4.70%
发文量
289
审稿时长
20 weeks
期刊介绍: Established in 2013, Bone Research is a newly-founded English-language periodical that centers on the basic and clinical facets of bone biology, pathophysiology, and regeneration. It is dedicated to championing key findings emerging from both basic investigations and clinical research concerning bone-related topics. The journal's objective is to globally disseminate research in bone-related physiology, pathology, diseases, and treatment, contributing to the advancement of knowledge in this field.
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