Identification of potential microRNAs involved in pathogenesis of venous thromboembolism (VTE): A meta analysis

IF 0.5 Q4 GENETICS & HEREDITY Human Gene Pub Date : 2024-10-11 DOI:10.1016/j.humgen.2024.201346

Sunanda Arya, Rashi Khare, Iti Garg, Swati Srivastava

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Abstract

Objective

MicroRNAs (MiRNAs) are master regulators of gene expression and have been suggested as potential diagnostic and therapeutic biomarkers in variety of complex diseases. Venous thromboembolism is a major cause of morbidity and mortality worldwide. Several miRNA expression studies have been conducted to identify miRNAs linked to VTE prognosis. These studies reveal that various miRNAs are significantly up-regulated and down-regulated in VTE patients in comparison to healthy controls. Present meta-analysis deliberate findings of such studies to identify most potential miRNA targets associated with VTE.

Methodology

Comprehensive literature review on Pubmed was conducted. Present analysis assessed 20 research article out of a total of 383 articles screened, based on inclusion and exclusion criteria. The up-regulated and down-regulated miRNAs obtained from selected research articles were subjected to meta-analysis. The differentially expressed miRNAs so obtained and were used for further bioinformatic analysis, including gene ontology and pathway analysis of their target genes, to study their potential involvement in VTE pathogenesis.

Results

Twenty articles selected for meta-analysis included a total of 808 patients. These included 26 up-regulated miRNAs and 11 down-regulated miRNAs. The meta-analysis based on Odds ratio suggested that one up-regulated miRNA (hsa-miR-1233-3p) and two down-regulated miRNAs (hsa-miR-103a-3p and hsa-miR-200c) returned significantly higher Odds compared to other miRNAs. Further bioinformatics analysis of their target genes revealed that these miRNAs target a large number of genes involved in cell adhesion, cell migration, endothelial activation and inflammation genes.

Conclusions

Three miRNAs, viz.; hsa-miR-1233-3p, hsa-miR-103a-3p and hsa-miR-200c may play a crucial role in VTE pathogenesis and has potential to serve as reliable diagnostic or therapeutic biomarkers for VTE.

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鉴定参与静脉血栓栓塞症（VTE）发病机制的潜在 microRNA：荟萃分析

目的微小核糖核酸（MiRNA）是基因表达的主要调控因子，被认为是各种复杂疾病的潜在诊断和治疗生物标志物。静脉血栓栓塞症是全球发病率和死亡率的主要原因。目前已开展了多项 miRNA 表达研究，以确定与 VTE 预后有关的 miRNA。这些研究显示，与健康对照组相比，VTE 患者体内的各种 miRNAs 有明显的上调和下调。本荟萃分析对这些研究结果进行了审议，以确定与 VTE 相关的大多数潜在 miRNA 靶点。根据纳入和排除标准，本分析从总共筛选出的 383 篇文章中评估了 20 篇研究文章。对所选研究文章中上调和下调的 miRNA 进行荟萃分析。获得的差异表达 miRNA 用于进一步的生物信息学分析，包括对其靶基因进行基因本体和通路分析，以研究它们在 VTE 发病机制中的潜在参与。其中包括 26 个上调的 miRNA 和 11 个下调的 miRNA。基于Odds比的荟萃分析表明，与其他miRNA相比，一个上调的miRNA（hsa-miR-1233-3p）和两个下调的miRNA（hsa-miR-103a-3p和hsa-miR-200c）的Odds比明显较高。结论三种 miRNA，即 hsa-miR-1233-3p、hsa-miR-103a-3p 和 hsa-miR-200c 可能在 VTE 发病机制中发挥关键作用，并有可能成为 VTE 的可靠诊断或治疗生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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