Dissemination and characterization of Escherichia coli resistant to extended-cephalosporins in feedlot lambs: A two-year two-population study

IF 2 3区农林科学 Q4 IMMUNOLOGY Comparative Immunology Microbiology and Infectious Diseases Pub Date : 2024-10-11 DOI:10.1016/j.cimid.2024.102258

Katia Suemi Gozi , Caroline Rodrigues da Silva , Marlon do Valle Barroso , Jessica Priscila Barboza , Juliana Regina Peiró , Jean-Yves Madec , Marisa Haenni , Luiz Claudio Nogueira Mendes , Mara Corrêa Lelles Nogueira , Tiago Casella

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Abstract

Dissemination of antimicrobial resistance in food animals is a One Health concern, but sheep production has been overlooked. This study aimed to explore the dissemination of Escherichia coli resistant to extended-spectrum cephalosporins (ESC) in feedlot lambs. Two pens were sampled on two occasions, and carcasses and other mammals living around were also tested. E. coli were recovered and antibiotic resistance determined. bla_CTX-M/CMY genes and their genetic localization were characterized. Whole genome sequencing (WGS) was performed to confirm clonal relationship. The most prevalent ESC-resistance genes in the 108 E. coli isolates were bla_CTX-M-55 (53.7 %), bla_CTX-M-2 (14.8 %) and bla_CMY-2 (13.9 %). Most bla_CTX-M-55 genes were found on the chromosome, but IncA/C, IncHI1, IncHI2 and IncF plasmids were also identified. Genetic diversity was observed even though ST6448 was by far the most frequent ST. WGS analysis showed high similarity among isolates recovered from feedlot lambs, animals in the surroundings and lambs’ carcasses, proving the clonal and plasmidic dissemination.

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饲养场羔羊中对扩展头孢菌素耐药的大肠埃希菌的传播和特征：一项为期两年的双群体研究

抗菌药耐药性在食用动物中的传播是 "一个健康 "关注的问题，但在绵羊生产中却被忽视了。本研究旨在探讨对广谱头孢菌素（ESC）耐药的大肠埃希菌在饲养场羔羊中的传播情况。研究人员对两个羊圈进行了两次采样，并对羔羊尸体和周围的其他哺乳动物进行了检测。对 blaCTX-M/CMY 基因及其基因定位进行了鉴定。进行了全基因组测序（WGS）以确认克隆关系。108 株大肠杆菌分离物中最常见的耐 ESC 基因是 blaCTX-M-55（53.7%）、blaCTX-M-2（14.8%）和 blaCMY-2（13.9%）。大多数 blaCTX-M-55 基因存在于染色体上，但也发现了 IncA/C、IncHI1、IncHI2 和 IncF 质粒。尽管 ST6448 是迄今为止最常见的 ST，但还是观察到了遗传多样性。WGS 分析表明，从饲养场羔羊、周围环境中的动物和羔羊尸体中分离出的分离物具有高度相似性，这证明了克隆和质粒传播。

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来源期刊

Comparative Immunology Microbiology and Infectious Diseases 医学-免疫学

CiteScore

4.60

自引率

0.00%

发文量

102

审稿时长

40 days

期刊介绍： Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine . The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.