Jiawei Wumei Wan alleviates renal fibrosis in diabetic nephropathy mice by regulating the PI3K/AKT/mTOR signaling pathway

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL Chinese Journal of Analytical Chemistry Pub Date : 2024-10-01 DOI:10.1016/j.cjac.2024.100446

Yijun PI , Xu HUANG , Chengji CUI , Fan LI , Wenjing ZHANG , Chunguang YI , Shoulin ZHANG

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Abstract

In this study, network pharmacological and experimental analyses were implemented to verify the pharmacodynamic effects of Jiawei Wumei Wan (JWWMW) in the treating renal fibrosis in diabetic nephropathy (DN) and investigate its mechanism of action. Spontaneous type II diabetic mice were used to simulate the clinical DN model. JWWMW improved renal fibrosis in DN mice. Additionally, analyses of the body mass, blood sugar, 24 h urine output of mice, kidney mass, pathological morphology of renal tissues, and biochemical indices showed that JWWMW exerted therapeutic effects. Bioinformatics analyses also predicted that JWWMW may play a role in the biosynthetic process and pathway of lipid and atherosclerosis, as well as a role in the AGE-RAGE signaling pathway in diabetic complications. Western blotting results verified the reduction in lipid production after JWWMW treatment. This was facilitated by regulating the PI3K/AKT/mTOR signal pathway, thus preventing and treating renal fibrosis in DN. This study describes a scientific and theoretical approach to the clinical application of JWWMW.

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加味乌梅丸通过调节PI3K/AKT/mTOR信号通路减轻糖尿病肾病小鼠的肾脏纤维化

本研究通过网络药理学和实验分析，验证了加味乌梅丸（JWWMW）治疗糖尿病肾病（DN）肾纤维化的药效学作用，并研究了其作用机制。实验采用自发Ⅱ型糖尿病小鼠模拟临床糖尿病肾病模型。JWWMW 可改善 DN 小鼠的肾脏纤维化。此外，对小鼠体重、血糖、24 小时尿量、肾脏质量、肾组织病理形态和生化指标的分析表明，JWWMW 具有治疗作用。生物信息学分析还预测，JWWMW可能在脂质和动脉粥样硬化的生物合成过程和途径中发挥作用，并在糖尿病并发症的AGE-RAGE信号通路中发挥作用。Western 印迹检测结果证实了 JWWMW 治疗后脂质生成的减少。通过调节 PI3K/AKT/mTOR 信号通路，从而预防和治疗 DN 肾纤维化。本研究描述了 JWWMW 临床应用的科学理论方法。

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来源期刊

Chinese Journal of Analytical Chemistry 化学-分析化学

CiteScore

3.60

自引率

25.00%

发文量

17223

审稿时长

35 days

期刊介绍： Chinese Journal of Analytical Chemistry(CJAC) is an academic journal of analytical chemistry established in 1972 and sponsored by the Chinese Chemical Society and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Its objectives are to report the original scientific research achievements and review the recent development of analytical chemistry in all areas. The journal sets up 5 columns including Research Papers, Research Notes, Experimental Technique and Instrument, Review and Progress and Summary Accounts. The journal published monthly in Chinese language. A detailed abstract, keywords and the titles of figures and tables are provided in English, except column of Summary Accounts. Prof. Wang Erkang, an outstanding analytical chemist, academician of Chinese Academy of Sciences & Third World Academy of Sciences, holds the post of the Editor-in-chief.