Electrochemical and optical biosensors for the detection of E. Coli

Abstract

E. coli is a common pathogenic microorganism responsible for numerous food and waterborne illnesses. Traditional detection methods often require long, multi-step processes and specialized equipment. Electrochemical and optical biosensors offer promising alternatives due to their high sensitivity, selectivity, and real-time monitoring capabilities. Recent advancements in sensor development focus on various techniques for detecting E. coli, including optical (fluorescence, colorimetric analysis, surface-enhanced Raman spectroscopy, surface plasmon resonance, localized surface plasmon resonance, chemiluminescence) and electrochemical (amperometric, voltammetry, impedance, potentiometric). Herein, the latest advancements in optical and electrochemical biosensors created for identifying E. coli with an emphasis on surface modifications employing nanomaterials and biomolecules are outlined in this review. Electrochemical biosensors exploit the unique electrochemical properties of E. coli or its specific biomolecules to generate a measurable signal. In contrast, optical biosensors rely on interactions between E. coli and optical elements to generate a detectable response. Moreover, optical detection has been exploited in portable devices such as smart phones and paper-based sensors. Different types of electrodes, nanoparticles, antibodies, aptamers, and fluorescence-based systems have been employed to enhance the sensitivity and specificity of these biosensors. Integrating nanotechnology and biorecognition (which bind to a specific region of the E. coli) elements has enabled the development of portable and miniaturized devices for on-site and point-of-care (POC) applications. These biosensors have demonstrated high sensitivity and offer low detection limits for E. coli detection. The convergence of electrochemical and optical technologies promises excellent opportunities to revolutionize E. coli detection, improving food safety and public health.