Antimicrobial activity of herbal biomass derived metabolites fabricated wound dressing material against microbial strains causing skin infection

Abstract

In the present study, anti-microbial activity of wound dressing material coated with Aloe vera and Azadirachta indica metabolites was investigated on pathogenic microbial strains associated with skin infection. Anti-microbial active metabolites extracted from the respective medicinal plant were fabricated on the wound dressing material by simple, in situ green science principles. Confirmation of phytochemicals fabrication on the fibre surface of the material was studied by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) Additionally, the impact of phyto-constituents fabrication on mechanical properties such as thickness and tensile strength was assessed. The anti-microbial efficacy of the fabricated dressing against pathogenic microorganisms (Candida albicans, Escherichia coli ATCC 25922, and Staphylococcus aureus ATCC 25923) was evaluated using agar diffusion assay. The release profile of phytochemical constituents from the fabricated wound dressing was investigated using ethanol and distilled water as solvents. The results showed that the phytochemical constituents were fabricated effectively on the wound dressing material and exhibiting notable growth inhibition potential against tested pathogenic strains. The release profile study revealed an initial burst release of phytochemical constituents followed by a controlled or sustained pattern. In conclusion, wound dressings infused with plant extracts present a promising approach for wound care applications and show potential for various healing-related purpose.