The role and mechanism of TgCWIN2-mediated changes of photo-assimilates in modulating early development of Torreya grandis seeds

IF 6.1 2区 生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2024-10-14 DOI:10.1016/j.plaphy.2024.109188
Jinwei Suo , Jiayue Zhong , Minmin Yang, Qianxi Li, Yuanyuan Hu, Weiwu Yu, Jingwei Yan, Jiasheng Wu
{"title":"The role and mechanism of TgCWIN2-mediated changes of photo-assimilates in modulating early development of Torreya grandis seeds","authors":"Jinwei Suo ,&nbsp;Jiayue Zhong ,&nbsp;Minmin Yang,&nbsp;Qianxi Li,&nbsp;Yuanyuan Hu,&nbsp;Weiwu Yu,&nbsp;Jingwei Yan,&nbsp;Jiasheng Wu","doi":"10.1016/j.plaphy.2024.109188","DOIUrl":null,"url":null,"abstract":"<div><div>Early seed development is vital for plant reproduction, but the processes behind this in gymnosperms like <em>Torreya grandis</em>, which has a low rate of normal early-developed seeds, are not well understood. To fill this knowledge gap, we embarked on a comprehensive investigation encompassing the morphology and phenology of seed development in <em>T. grandis</em>. Using the <sup>13</sup>C labelling analysis, coupled with leaf removal and seed thinning treatments, we observed a substantial increase in the content of photo-assimilate, an almost 10% increase in sucrose content under seed thinning treatments, thereby leading to an increase in the proportion of normal early-developed seeds, reaching 15%. Concurrently, through the integration of multi-omics analyses and transient overexpression validation, we identified cell wall invertase coding gene, <em>TgCWIN2</em>, which plays a pivotal role in sucrose cleavage during the early development of <em>T. grandis</em> seeds. Further gene co-expression, dual-luciferase assay, and yeast one-hybrid assay revealed that TgWRKY31 was a candidate regulator of <em>TgCWIN2</em>, positively influencing its expression by direct binding to the <em>TgCWIN2</em> promoter. Notably, <em>TgWRKY31</em> transient overexpression substantially enhances the expression of <em>TgCWIN2</em>, thereby contributing to a higher proportion of normal early-developed seeds. Our findings not only provide a comprehensive understanding of the underlying mechanisms governing the early development of <em>T. grandis</em> seeds, but are also essential for establishing strategies to enhance early seed development and improve yield.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942824008568","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Early seed development is vital for plant reproduction, but the processes behind this in gymnosperms like Torreya grandis, which has a low rate of normal early-developed seeds, are not well understood. To fill this knowledge gap, we embarked on a comprehensive investigation encompassing the morphology and phenology of seed development in T. grandis. Using the 13C labelling analysis, coupled with leaf removal and seed thinning treatments, we observed a substantial increase in the content of photo-assimilate, an almost 10% increase in sucrose content under seed thinning treatments, thereby leading to an increase in the proportion of normal early-developed seeds, reaching 15%. Concurrently, through the integration of multi-omics analyses and transient overexpression validation, we identified cell wall invertase coding gene, TgCWIN2, which plays a pivotal role in sucrose cleavage during the early development of T. grandis seeds. Further gene co-expression, dual-luciferase assay, and yeast one-hybrid assay revealed that TgWRKY31 was a candidate regulator of TgCWIN2, positively influencing its expression by direct binding to the TgCWIN2 promoter. Notably, TgWRKY31 transient overexpression substantially enhances the expression of TgCWIN2, thereby contributing to a higher proportion of normal early-developed seeds. Our findings not only provide a comprehensive understanding of the underlying mechanisms governing the early development of T. grandis seeds, but are also essential for establishing strategies to enhance early seed development and improve yield.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
TgCWIN2 介导的光同化物变化在调节香榧种子早期发育中的作用和机制
种子的早期发育对植物的繁殖至关重要,但对于像大香榧这样种子早期发育正常率较低的裸子植物来说,其背后的发育过程还不甚了解。为了填补这一知识空白,我们对大叶香榧种子发育的形态学和物候学进行了全面调查。利用 13C 标记分析,并结合摘叶和疏种处理,我们观察到光同化物的含量大幅增加,疏种处理下的蔗糖含量增加了近 10%,从而导致正常早期发育种子的比例增加,达到 15%。同时,通过整合多组学分析和瞬时过表达验证,我们发现了细胞壁转化酶编码基因 TgCWIN2,它在鹅掌楸种子早期发育过程中的蔗糖裂解中起着关键作用。进一步的基因共表达、双荧光素酶检测和酵母单杂交检测发现,TgWRKY31是TgCWIN2的候选调控因子,通过直接与TgCWIN2启动子结合对其表达产生积极影响。值得注意的是,TgWRKY31的瞬时过表达大大提高了TgCWIN2的表达,从而使早期发育正常的种子比例更高。我们的研究结果不仅让人们全面了解了鹅掌楸种子早期发育的基本机制,而且对于制定加强种子早期发育和提高产量的策略也至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
期刊最新文献
Riboflavin improves postharvest cold tolerance in zucchini fruit inducing non-enzymatic antioxidant response and phenolic metabolism Infection of tomato plants by tomato yellow leaf curl virus (TYLCV) potentiates the ethylene and salicylic acid pathways to fend off root-knot nematode (Meloidogyne incognita) parasitism Effects of water deficit on two cultivars of Hibiscus mutabilis: A comprehensive study on morphological, physiological, and metabolic responses Effect of green and UVA spectra, and pre-harvest treatments on biomass and metabolite yields of indoor cultivated stevia rebaudiana Combining transcriptome and metabolome analyses to reveal the response of maize roots to Pb stress
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1