Plant Physiology and Biochemistry最新文献

Endogenous salicylic acid contributes to cadmium tolerance in Monochoria korsakowii through upregulation of photosynthetic efficiency, antioxidant capacity, and chelators accumulation

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-04-22 DOI: 10.1016/j.plaphy.2025.109940

Qianqian Zheng , Lu Yang , Jianpan Xin, Chu Zhao, Yan Li, Runan Tian

Exogenous salicylic acid (SA) enhances plant tolerance to cadmium (Cd) stress by preserving chlorophyll, stabilizing osmoprotectants, and upregulating antioxidant activity alongside the ASA-GSH system. However, the role of endogenous SA in plant tolerance to Cd stress remains poorly understood. Therefore, we cultivated Monochoria korsakowii hydroponically and sprayed the SA biosynthesis inhibitors (2-aminoindane-2-phosphonic acid and 1-aminobenzotriazole) in an attempt to explore the correlation between endogenous SA and other Cd tolerance mechanisms. Compared with control, 0.3 mM Cd treatment induced reductions of net photosynthetic rate (Pn), total chlorophyll (T Chl), catalase (CAT), and soluble protein (SP), while malondialdehyde increased. To mitigate Cd toxicity, M. korsakowii upregulated peroxidase (POD), superoxide dismutase (SOD), glutathione reductase (GR), ascorbic acid (ASA), nonprotein thiols (NPT), phytochelatin (PC), and proline. High concentrations of SA inhibitors exacerbated Cd-induced oxidative damage and suppressed these tolerance mechanisms. Compared with T4, T6 plants exhibited marked reductions in Pn, T Chl, CAT, POD, SOD, GSH, GR, ASA, ascorbate peroxidase, NPT, PCs, SP, and translocation factors. Concurrently, T6 plants sprayed with SA inhibitors exhibited suppressed SA, methyl salicylate, and zeatin accumulation, contrasting with heightened jasmonic acid and abscisic acid concentrations. We propose that endogenous SA is crucial for preserving the photosynthetic apparatus, activating the antioxidant system, and promoting the accumulation of chelators and SP in M. korsakowii under Cd stress. Furthermore, endogenous SA may function synergistically with methyl salicylate and zeatin to regulate plant physiological responses to Cd. This study provides valuable insights into the Cd tolerance mechanisms in M. korsakowii.

外源水杨酸（SA）可通过保护叶绿素、稳定渗透保护剂和提高 ASA-GSH 系统的抗氧化活性来增强植物对镉（Cd）胁迫的耐受性。然而，人们对内源 SA 在植物耐受镉胁迫中的作用仍知之甚少。因此，我们水培并喷洒了SA生物合成抑制剂（2-氨基茚满-2-膦酸和1-氨基苯并三唑），试图探索内源SA与其他镉耐受机制之间的相关性。与对照组相比，0.3 mM Cd 处理导致净光合速率（Pn）、总叶绿素（T Chl）、过氧化氢酶（CAT）和可溶性蛋白（SP）降低，丙二醛增加。为了减轻镉的毒性，M. korsakowii 提高了过氧化物酶（POD）、超氧化物歧化酶（SOD）、谷胱甘肽还原酶（GR）、抗坏血酸（ASA）、非蛋白质硫醇（NPT）、植物螯合素（PC）和脯氨酸的浓度。高浓度的 SA 抑制剂加剧了 Cd 诱导的氧化损伤，并抑制了这些耐受机制。与 T4 相比，T6 植物的 Pn、T Chl、CAT、POD、SOD、GSH、GR、ASA、抗坏血酸过氧化物酶、NPT、PC、SP 和易位因子明显减少。同时，喷洒了 SA 抑制剂的 T6 植株表现出 SA、水杨酸甲酯和玉米素积累受到抑制，而茉莉酸和脱落酸浓度则有所提高。我们认为，在镉胁迫下，内源 SA 对保护光合装置、激活抗氧化系统以及促进螯合剂和 SP 的积累至关重要。此外，内源 SA 可能与水杨酸甲酯和玉米素协同调节植物对镉的生理反应。这项研究为了解 M. korsakowii 的镉耐受机制提供了宝贵的见解。

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引用次数: 0

Complex regulatory network of ZmbZIP54-mediated Pb tolerance in maize

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-04-21 DOI: 10.1016/j.plaphy.2025.109945

Fengxia Hou , Yuru Liang , Mengxiang Sang , Guixiang Zhao , Jing Song , Peng Liu , Chaoying Zou , Zhong Chen , Langlang Ma , Yaou Shen

Lead (Pb) is highly toxic and widely distributed in the soil, causing adverse effects on plant growth and yield formation. Herein, the combination of transmission electron microscope (TEM), energy dispersive X-ray Spectroscopy (EDS), and comparative transcriptome analyses was conducted to reveal the cytological mechanism and regulatory network of in the ZmbZIP54-mediated Pb tolerance in maize. As results, ZmbZIP54 helped in Pb²⁺ retention in the cell wall and intercellular space, inhibiting Pb²⁺ entering the cells and reducing its toxic effects on cell ultrastructure. Meanwhile, ZmbZIP54 was involved in the transition between the HCl-extracted and CH₃COOH-extracted Pb speciations. At the molecular level, ZmbZIP54 affected the macromolecule metabolism, thus decreasing Pb accumulation in the roots. Moreover, ZmZIFL1 and NRT1/PTR were the direct targets of ZmbZIP54, which participated in heavy metal binding, nitrogen uptaking, and IAA transport and thus mediated Pb transport, Pb speciation transition, and antioxidant enzyme activation. Collectively, we proposed a model to explain the complex regulatory network mediated by ZmbZIP54 and its target genes in maize tolerance to Pb stress.

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引用次数: 0

Comparative analysis of rapid alkalinization factor peptide-triggered plant immunity in citrus and closely related species

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-04-21 DOI: 10.1016/j.plaphy.2025.109941

Wenzhong Shen , Mengying Yuan , Lijuan Chen , Xinxin Zhang

Rapid alkalinization factors (RALFs) are plant-derived, cysteine-rich small peptides that play crucial roles in plant development and immunity. However, their function in citrus immunity remains unexplored. This study systematically identified RALF family members in sweet orange, a cultivated citrus species, and five closely related species known for their strong resistance to citrus diseases. Most species contained 13 RALF genes, and their corresponding RALF orthologs exhibited minimal variation in gene structure, sequence identity, conserved domains, and motifs. However, differences in cis-acting elements within promoter regions led to differences in their expression patterns under biotic stress. Compared with sweet orange, citrus-related species exhibited a stronger and more widespread induction of RALF genes. Additionally, RALF peptides, particularly RALF11, triggered more robust immune responses in these species, including ROS bursts, mitogen-activated protein kinase activation, and the upregulation of defense-related genes. Furthermore, the basal expression levels of CrRLK1Ls, the RALF receptor homologs, were higher in citrus-related species than in sweet orange. Amplifying the RALF-CrRLK1L pathway through CrRLK1L overexpression and RALF peptide application significantly enhanced sweet orange resistance to citrus canker. These findings suggest that citrus-related species have endured selective pressure, leading to greater promoter sequence diversity, which is conducive to fine-tune gene expression in response to environmental challenges.

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引用次数: 0

Competitive ion uptake and transcriptional regulation as a coordinated dual mechanism of NaCl-mediated cadmium detoxification in Suaeda salsa

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-04-20 DOI: 10.1016/j.plaphy.2025.109939

Tian Li , Xiangna Yang , Haotian Sun , Hao Jing , Sinuo Bao , Yanfeng Hu , Wei Shi , Honglei Jia , Jisheng Li

Cadmium (Cd), a highly toxic heavy metal, severely inhibits plant growth. Salt alleviates Cd stress in halophytes, however, the molecular mechanisms governing salt-mediated regulation of Cd toxicity remain poorly understood. This study elucidates the protective mechanism of NaCl in Cd-stressed Suaeda salsa seedlings. Cd exposure suppressed seedling growth and induced membrane lipid peroxidation. Conversely, NaCl application not only maintained normal growth but also effectively ameliorated Cd-induced phytotoxicity, potentially through osmotic adjustment mechanisms. Notably, using ion flux analysis, we found that NaCl attenuated Cd²⁺ influx into root epidermal cells, thereby enhancing Cd resistance. Pharmacological inhibition studies confirmed that Na ⁺ competitively inhibits Cd²⁺ uptake through shared channels/transporters. Furthermore, RT-qPCR gene expression profiling revealed that NaCl coordinately activated both ionic compartmentalization and efflux systems through upregulating plasma membrane-localized SsSOS1 and tonoplast-associated SsNHX1 for Na ⁺ extrusion and vacuolar sequestration, enhancing Cd²⁺ compartmentalization via SsCAX and SsVHA-B mediated transport and maintaining cellular homeostasis through SsHKT1 and SsPIP-mediated regulation of water and K⁺ balance, or indirectly inhibit Cd²⁺ influx. It reveals that salt weakens Cd²⁺ influx and enhances Cd tolerance by activating a coordinated gene regulatory network in Suaeda salsa. This finding offers valuable insights into phytoremediation strategies for enhancing crop resilience in Cd-contaminated saline soils.

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引用次数: 0

MYB transcription factor MdMYB44 positively regulates fruit crispness by directly activating the expression of pectin methylesterase MdMPE3 in apple

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-04-17 DOI: 10.1016/j.plaphy.2025.109936

Ling Yang , Jiali He , Sijun Qin , Xiaojing Li , Xiaodi Wang , Deguo Lyu

Crispness, a key attribute of fruit texture quality, is a critical determinant of apple commercial value. Pectin, a major component of the cell wall, plays a vital role in maintaining cell structure, turgor pressure, and mechanical support, with pectin methylesterase (PMEs, EC 3.1.1.11) mediating pectin modification during cell wall remodeling. In this study, we identified two genes, MdMYB44 and MdMPE3 (pectin methylesterase 3), that regulate apple fruit crispness. Through Y1H, EMSA, ChIP-qPCR, and transient expression assays, we demonstrated that the MYB transcription factor MdMYB44 directly enhances the expression of MdMPE3 by binding to its promoter. These results indicate that MdMYB44 acts as a positive regulator of fruit crispness by activating MdMPE3 transcription. Our findings provide new insights into the molecular mechanisms by which MYB transcription factors and pectin methylesterase influence apple fruit texture, enriching our understanding of the regulation of fruit crispness.

脆度是果实质地质量的一个关键属性，是决定苹果商业价值的关键因素。果胶是细胞壁的主要成分，在维持细胞结构、张力压力和机械支撑方面起着至关重要的作用，果胶甲基酯酶（PMEs，EC 3.1.1.11）在细胞壁重塑过程中介导果胶的修饰。在这项研究中，我们发现了两个调控苹果果实脆度的基因：MdMYB44 和 MdMPE3（果胶甲基酯酶 3）。通过 Y1H、EMSA、ChIP-qPCR 和瞬时表达测定，我们证明了 MYB 转录因子 MdMYB44 通过与其启动子结合直接增强了 MdMPE3 的表达。这些结果表明，MdMYB44 通过激活 MdMPE3 的转录而成为果实脆度的正向调节因子。我们的研究结果为了解 MYB 转录因子和果胶甲基酯酶影响苹果果实质地的分子机制提供了新的视角，丰富了我们对果实脆度调控的认识。

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引用次数: 0

Identification of PP2C gene family in moso bamboo (Phyllostachys edulis) and function analysis of PhePP2CA13

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-04-17 DOI: 10.1016/j.plaphy.2025.109929

Qihan Lin, Xin Shan, Qin Si, Yidong Liu, Min Wu

The protein phosphatase 2C (PP2C) gene family plays an important role in plant growth and development and resistance to abiotic stresses. In our research, the PP2C family containing 103 genes was comprised eleven distinct subfamilies in moso bamboo (Phyllostachys edulis). PP2C gene family mainly expand through segmental duplications and basically underwent purifying selection in moso bamboo. PP2CA subfamily, the most extensively studied subfamily, carried 19 members in moso bamboo PP2CA subfamily. The cis-acting elements analysis and expression patterns of PhePP2CA genes indicated their potential functions in response to abiotic stress. Next, we gained the lines of transgenic PhePP2CA13-overexpressing plants. Overexpression of PhePP2CA13 significantly decreased tolerance to drought stress, along with a lower germination rate and suppressed root length under drought treatment. Moreover, OE lines also reduced the sensitivity to exogenous ABA in OE lines. More importantly, yeast one-hybrid assays demonstrated that PhePP2CA13 acted downstream of PhebZIP47, and Dual-luciferase assays further showed that the expression of PhePP2CA13 was inhibited by PhebZIP47. This study enriched the knowledge of PP2CA genes and provided genetic resources for stress resistant breeding in moso bamboo.

{"title":"Identification of PP2C gene family in moso bamboo (Phyllostachys edulis) and function analysis of PhePP2CA13","authors":"Qihan Lin, Xin Shan, Qin Si, Yidong Liu, Min Wu","doi":"10.1016/j.plaphy.2025.109929","DOIUrl":"10.1016/j.plaphy.2025.109929","url":null,"abstract":"<div><div>The protein phosphatase 2C (PP2C) gene family plays an important role in plant growth and development and resistance to abiotic stresses. In our research, the PP2C family containing 103 genes was comprised eleven distinct subfamilies in moso bamboo (<em>Phyllostachys edulis</em>). <em>PP2C</em> gene family mainly expand through segmental duplications and basically underwent purifying selection in moso bamboo. PP2CA subfamily, the most extensively studied subfamily, carried 19 members in moso bamboo PP2CA subfamily. The <em>cis</em>-acting elements analysis and expression patterns of <em>PhePP2CA</em> genes indicated their potential functions in response to abiotic stress. Next, we gained the lines of transgenic PhePP2CA13-overexpressing plants. Overexpression of <em>PhePP2CA13</em> significantly decreased tolerance to drought stress, along with a lower germination rate and suppressed root length under drought treatment. Moreover, OE lines also reduced the sensitivity to exogenous ABA in OE lines. More importantly, yeast one-hybrid assays demonstrated that PhePP2CA13 acted downstream of PhebZIP47, and Dual-luciferase assays further showed that the expression of <em>PhePP2CA13</em> was inhibited by PhebZIP47. This study enriched the knowledge of <em>PP2CA</em> genes and provided genetic resources for stress resistant breeding in moso bamboo.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109929"},"PeriodicalIF":6.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CpHSFA2 isolated from a wild native Carica papaya genotype, with potential to confer tolerance to the combined effect of drought stress and heat shock

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-04-17 DOI: 10.1016/j.plaphy.2025.109925

Yessica Bautista-Bautista , Gabriela Fuentes , Sergio García-Laynes , Felipe Alonso Barredo-Pool , Santy Peraza-Echeverria , Jorge M. Santamaría

Many papaya producing regions are repeatedly affected by drought and high temperatures. In the present study, we investigated the individual effect of heat shock (HS), water deficit stress (WDS) and the combined effect of both types of stress (WD + HS), on the physiological performance of two contrasting papaya genotypes (Maradol and Wild). In all cases, water relations, membrane integrity, gas exchange, photochemical state of PSII and RELs of three Carica papaya transcription factors (CpHsfA1d, CpHsfA2 and CpHsfB3, in both a Wild-native genotype collected from an undisturbed site in its center of origin (Yucatán, Mexico; Wild), as well as in a commercial cultivar (Maradol). Results showed that both papaya genotypes have different physiological and molecular mechanisms to cope with individual stress and combined stresses. Wild (W) genotype exhibited greater tolerance to the three types of stresses than the commercial genotype (M), which correlates with the fact that W also showed higher relative expression levels (REL) in the three CpHsf studied: CpHsfA1d, CpHsfA2 and CpHsfB3 than M. REL of CpHsfA2 was particularly high in the HS and in the combined WD + HS treatment, as well as during the recovery phase of the WDS treatment. CpHSFA2 was then selected for further analysis of subcellular localization, finding that it accumulates in the membrane and nucleus. Taken together, it seems that CpHsfA2 plays an important role in the response to HS and WD + HS stress. Therefore, CpHsfA2 gene from the W genotype could be important to eventually improve tolerance to high temperatures and drought in commercial papaya cultivars.

许多木瓜产区反复受到干旱和高温的影响。在本研究中，我们研究了热休克（HS）、水分亏缺胁迫（WDS）和两种胁迫（WD + HS）对两种不同木瓜基因型（Maradol 和 Wild）生理表现的单独影响。在所有情况下，从原产地中心（墨西哥尤卡坦州；Wild）未受干扰地点采集的野生原生基因型和商业栽培品种（Maradol）的水分关系、膜完整性、气体交换、PSII的光化学状态和三种木瓜转录因子（CpHsfA1d、CpHsfA2和CpHsfB3）的RELs都受到了影响。结果表明，这两种木瓜基因型具有不同的生理和分子机制来应对单独的压力和综合压力。野生基因型（W）比商业基因型（M）对三种胁迫表现出更大的耐受性，这与野生基因型在所研究的三种 CpHsf 中也表现出更高的相对表达水平（REL）有关：在 HS 处理、WD + HS 组合处理以及 WDS 处理的恢复阶段，CpHsfA2 的相对表达水平尤其高。然后选择 CpHSFA2 进一步分析其亚细胞定位，发现它在膜和细胞核中积累。综上所述，CpHsfA2似乎在对HS和WD + HS胁迫的响应中起着重要作用。因此，来自 W 基因型的 CpHsfA2 基因可能对最终提高商品番木瓜栽培品种对高温和干旱的耐受性具有重要意义。

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引用次数: 0

Light-induced CsCV triggers chloroplast degradation by destabilizing photosystem proteins in tea plant

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-04-17 DOI: 10.1016/j.plaphy.2025.109926

Xin Zhang , Xiaobei Huang , Zhijun Wu

Excess light induces chloroplast degradation in plants, leading to decreased photosynthetic efficiency and an albino leaf phenotype. However, the molecular mechanism underlying this process remains unclear, especially in perennial crops like tea plant. This study investigated the effects of relatively strong light (SL, 240 μmol m⁻²·s⁻¹) on chloroplast ultrastructure and metabolites in the light-sensitive tea germplasm Nanchuan Dachashu (Camellia nanchuanica). Continuous exposure to SL resulted in abnormal chloroplast structure characterized by extensive vacuolation. SL also significantly decreased the levels of chlorophyll (−60.30 %), carotenoids (−88.29 %), free amino acids (−23.97 %), and caffeine (−41.15 %) compared to relatively weak light (WL, 15 μmol m⁻²·s⁻¹). Transcriptome analysis and RT-qPCR revealed that the chloroplast vesiculation gene CsCV was significantly up-regulated under SL, with promoter analysis showing more light-responsive elements in CsCV compared to another light-responsive gene, CsNBR1. Overexpression of CsCV in Arabidopsis caused stunted growth and accelerated leaf senescence, with the most affected line showing decreases in chlorophyll and carotenoid contents of 24.97 % and 17.39 %, respectively. Conversely, silencing CsCV in tea plants using antisense oligodeoxynucleotides (asODNs) for 3 days increased chlorophyll and carotenoid levels by 15.98 % and 18.35 %, respectively. Bimolecular fluorescence complementation (BiFC) assays and in protein-protein docking simulations demonstrated that CsCV interacts with the photosystem proteins CsLhca1, CsLhcb4, and CsPsaL through its conserved C-terminal region, suggesting CsCV may trigger chloroplast degradation by destabilizing the photosynthetic apparatus under SL. These findings provide mechanistic insights into light-induced chloroplast degradation in tea plants and highlight CsCV as a potential target for improving crop stress tolerance.

{"title":"Light-induced CsCV triggers chloroplast degradation by destabilizing photosystem proteins in tea plant","authors":"Xin Zhang , Xiaobei Huang , Zhijun Wu","doi":"10.1016/j.plaphy.2025.109926","DOIUrl":"10.1016/j.plaphy.2025.109926","url":null,"abstract":"<div><div>Excess light induces chloroplast degradation in plants, leading to decreased photosynthetic efficiency and an albino leaf phenotype. However, the molecular mechanism underlying this process remains unclear, especially in perennial crops like tea plant. This study investigated the effects of relatively strong light (SL, 240 μmol m<sup>−2</sup>·s<sup>−1</sup>) on chloroplast ultrastructure and metabolites in the light-sensitive tea germplasm Nanchuan Dachashu (<em>Camellia nanchuanica</em>). Continuous exposure to SL resulted in abnormal chloroplast structure characterized by extensive vacuolation. SL also significantly decreased the levels of chlorophyll (−60.30 %), carotenoids (−88.29 %), free amino acids (−23.97 %), and caffeine (−41.15 %) compared to relatively weak light (WL, 15 μmol m<sup>−2</sup>·s<sup>−1</sup>). Transcriptome analysis and RT-qPCR revealed that the chloroplast vesiculation gene <em>CsCV</em> was significantly up-regulated under SL, with promoter analysis showing more light-responsive elements in <em>CsCV</em> compared to another light-responsive gene, <em>CsNBR1</em>. Overexpression of <em>CsCV</em> in <em>Arabidopsis</em> caused stunted growth and accelerated leaf senescence, with the most affected line showing decreases in chlorophyll and carotenoid contents of 24.97 % and 17.39 %, respectively. Conversely, silencing <em>CsCV</em> in tea plants using antisense oligodeoxynucleotides (asODNs) for 3 days increased chlorophyll and carotenoid levels by 15.98 % and 18.35 %, respectively. Bimolecular fluorescence complementation (BiFC) assays and in protein-protein docking simulations demonstrated that CsCV interacts with the photosystem proteins CsLhca1, CsLhcb4, and CsPsaL through its conserved C-terminal region, suggesting CsCV may trigger chloroplast degradation by destabilizing the photosynthetic apparatus under SL. These findings provide mechanistic insights into light-induced chloroplast degradation in tea plants and highlight <em>CsCV</em> as a potential target for improving crop stress tolerance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109926"},"PeriodicalIF":6.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated transcriptomics and metabolomics analyses reveal jasmonic acid metabolic pathways for improving the chilling tolerance in cotton seedlings

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-04-17 DOI: 10.1016/j.plaphy.2025.109935

Luyao Wang , Yaping Jiang , Yupeng Hao , Li Yu , Shengjun Zhao , Hongyu Wu , Xuan Long , Zhiyuan Zhang , Ting Zhao , Shiwei Geng , Xueying Guan

Cotton (Gossypium spp.) originated in tropical and subtropical regions, spreading to higher latitudes through domestication while retaining thermophilic characteristics. Xinjiang, a major cotton-producing area in China, frequently experiences ‘late spring cold snaps’ due to its location, causing chilling injury during critical sowing periods. Current research on cotton chilling stress primarily focuses on physiological studies such as evaluations of chilling stress and biochemical indices but lacks systematic investigation into the difference among varieties. Phenotypic screening across seed germination, cotyledon, and seedling stages identified upland cotton (Gossypium hirsutum) cultivar, Junmian1 exhibits superior cold tolerance relative to the sensitive genotype C1470. Under chilling stress, Junmian1 protects chloroplasts and other cellular structures in its first true leaf to survive the chilling stress. Weighted gene co-expression network analysis (WGCNA) analysis pinpointed Module Brown as a chilling-tolerance responsive hub, with subsequent validation via virus-induced gene silencing (VIGS) confirming the regulatory roles of GhRBL (Ribulose-bisphosphate carboxylase), GhGI (GIGANTEA), and lncRNA MSTR.1631 in cold tolerance. Additionally, integrated metabolomic and transcriptomic analyses demonstrated that jasmonic acid plays a crucial role in enhancing cotton's chilling tolerance at seedling stage. The primary difference in chilling tolerance between Junmian1 and C1470 is attributed to the signaling efficiency of the jasmonic acid synthesis and metabolism pathways. These findings establish JA metabolic engineering as a viable approach for enhancing cold resilience in early-stage cotton seedlings.

{"title":"Integrated transcriptomics and metabolomics analyses reveal jasmonic acid metabolic pathways for improving the chilling tolerance in cotton seedlings","authors":"Luyao Wang , Yaping Jiang , Yupeng Hao , Li Yu , Shengjun Zhao , Hongyu Wu , Xuan Long , Zhiyuan Zhang , Ting Zhao , Shiwei Geng , Xueying Guan","doi":"10.1016/j.plaphy.2025.109935","DOIUrl":"10.1016/j.plaphy.2025.109935","url":null,"abstract":"<div><div>Cotton (<em>Gossypium</em> spp.) originated in tropical and subtropical regions, spreading to higher latitudes through domestication while retaining thermophilic characteristics. Xinjiang, a major cotton-producing area in China, frequently experiences ‘late spring cold snaps’ due to its location, causing chilling injury during critical sowing periods. Current research on cotton chilling stress primarily focuses on physiological studies such as evaluations of chilling stress and biochemical indices but lacks systematic investigation into the difference among varieties. Phenotypic screening across seed germination, cotyledon, and seedling stages identified upland cotton (<em>Gossypium hirsutum</em>) cultivar, Junmian1 exhibits superior cold tolerance relative to the sensitive genotype C1470. Under chilling stress, Junmian1 protects chloroplasts and other cellular structures in its first true leaf to survive the chilling stress. Weighted gene co-expression network analysis (WGCNA) analysis pinpointed Module Brown as a chilling-tolerance responsive hub, with subsequent validation via virus-induced gene silencing (VIGS) confirming the regulatory roles of <em>GhRBL</em> (Ribulose-bisphosphate carboxylase), <em>GhGI</em> (GIGANTEA), and lncRNA <em>MSTR.1631</em> in cold tolerance. Additionally, integrated metabolomic and transcriptomic analyses demonstrated that jasmonic acid plays a crucial role in enhancing cotton's chilling tolerance at seedling stage. The primary difference in chilling tolerance between Junmian1 and C1470 is attributed to the signaling efficiency of the jasmonic acid synthesis and metabolism pathways. These findings establish JA metabolic engineering as a viable approach for enhancing cold resilience in early-stage cotton seedlings.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109935"},"PeriodicalIF":6.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription factor WUSCHEL-related homeobox (WOX) underground revelations: Insights into plant root development 转录因子 WUSCHEL 相关同源框（WOX）的地下启示：植物根系发育的启示

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-04-17 DOI: 10.1016/j.plaphy.2025.109928

Yuzheng Deng , Yongjie Zhu , Wanyi Su , Meiling Zhang , Weibiao Liao

Plant roots are essential for nutrient and water uptake and play a crucial role in plant growth and development. The development of roots is a complex process regulated by numerous factors, among which transcription factors (TFs) like WUSCHEL-related homeobox (WOX) have an essential function. The importance of WOXs in root development cannot be overstated. They act as key regulators in maintaining the balance between cell proliferation and differentiation and ensure the proper formation and function of root tissues. This review comprehensively presents the roles of WOXs in various root development aspects across multiple plant species, including primary, lateral, adventitious, and crown root development, as well as root hair, rhizoid formation, de novo root regeneration, and root apical meristem maintenance. We also discuss how WOXs regulate root development through various mechanisms in different plant species. Overall, this review provides comprehensive insights into the complex regulatory networks governing plant root growth and the importance of WOXs therein. Understanding WOXs in root development can help improve crop root architecture and stress tolerance and provide insights into the regulatory networks of plant root growth, contributing to plant breeding and agricultural productivity.

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引用次数: 0