Wenhan Kang, Jiukai Zhang, Ning Yu, Liyang Wei, Ying Chen
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引用次数: 0
Abstract
Pru p 7 (also named Peamaclein) is a member of the gibberellin-regulated protein family, which is the latest foodborne allergenic protein identified in peach. In this paper, the prokaryotic expression and identification of Pru p 7 were performed, and the protein properties, structure, and homology were analyzed. In addition, a preliminary screening of B-cell linear epitopes of Pru p 7 was performed by the bioinformatics software prediction method, and three epitopes were identified using slot-blot immune microarray assay combined with an immune score matrix (P-1, AA16-21, AGYQER; P-2, AA40-46, TYGNKDE; P-3, AA52-59, DLKNSKGN). Moreover, the electrostatic potential of these epitopes was analyzed, and the stability after ultrahigh pressure treatment was also verified. Finally, the amino acids that play key immune roles in the epitopes were obtained by amino acid mutations. These results may contribute to the further understanding of Pru p 7 and the prevention of peach allergy.
Pru p 7（又名 Peamaclein）是赤霉素调控蛋白家族的一员，是最新发现的桃子食源性过敏原蛋白。本文对 Pru p 7 进行了原核表达和鉴定，并对其蛋白质性质、结构和同源性进行了分析。此外，还利用生物信息学软件预测方法对 Pru p 7 的 B 细胞线性表位进行了初步筛选，并通过槽斑免疫芯片检测结合免疫评分矩阵确定了三个表位（P-1，AA16-21，AGYQER；P-2，AA40-46，TYGNKDE；P-3，AA52-59，DLKNSKGN）。此外，还分析了这些表位的静电位势，并验证了超高压处理后的稳定性。最后，通过氨基酸突变获得了在表位中起关键免疫作用的氨基酸。这些结果可能有助于进一步了解 Pru p 7 和预防桃过敏。
期刊介绍：
The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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