Maternal Broccoli Powder Intake Ameliorates Insulin Resistance and Inflammation via AMPK/mTOR Pathway in the Livers of High-Fructose-Fed Male Rat Offspring Exposed to Maternal Protein Restriction

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Molecular Nutrition & Food Research Pub Date : 2024-10-17 DOI:10.1002/mnfr.202400472

Anishma Karmacharya, Shiho Kasai, Yuuka Mukai, Shin Sato

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Abstract

Scope

Sub-optimal prenatal conditions such as maternal undernutrition during pregnancy and lactation posit high risks of adult metabolic diseases. High fructose intake causes insulin resistance and liver inflammation contributing to metabolic diseases. However, food-based preventive measure for these metabolic diseases in the offspring is under-researched. This study aims to investigate the effect of maternal broccoli powder (BP) intake during lactation on insulin resistance and liver inflammation in high-fructose-diet-fed adult male offspring exposed to maternal protein restriction.

Methods and results

Pregnant Wistar rats are provided normal protein (NP) or low protein (LP) diets and 0% or 0.74% BP-containing NP diets and 0% or 0.74% BP-containing LP diet during lactation. At weaning, offspring receiving water (W) or 10% fructose solution (Fr) are assigned into six groups: NP/NP/W, NP/NP/Fr, NP/NPBP/Fr, LP/LP/W, LP/LP/Fr, and LP/LPBP/Fr. At week 13, plasma insulin, macrophage infiltration, activated protein kinase (AMPK) and mechanistic target of rapamycin (mTOR) phosphorylation, and autophagy flux markers are examined. LP/LPBP/Fr shows lower insulin levels and Homeostatic model assessment for insulin resistance (HOMA-IR) values than LP/LP/Fr. Liver macrophage infiltration are decreased in LP/LPBP/Fr. LP/LPBP/Fr exhibits upregulated AMPK phosphorylation, downregulated mTOR phosphorylation, and increased Microtubule-associated protein1A/1B-light chain 3B-II (LC3B-II) levels.

Conclusion

Maternal BP intake during lactation ameliorates insulin resistance and inflammation in the livers of adult offspring on a high-fructose diet from LP mothers.

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母体摄入西兰花粉能通过AMPK/mTOR通路改善暴露于母体蛋白质限制的高果糖雄性大鼠后代肝脏中的胰岛素抵抗和炎症。

SCOPES 产前条件欠佳，如孕期和哺乳期母体营养不良，会导致成人代谢性疾病的高风险。高果糖摄入会导致胰岛素抵抗和肝脏炎症，从而引发代谢性疾病。然而，以食物为基础预防后代患上这些代谢性疾病的措施还未得到充分研究。本研究旨在探讨哺乳期母体摄入西兰花粉（BP）对暴露于母体蛋白质限制的高果糖膳食成年雄性后代的胰岛素抵抗和肝脏炎症的影响。断奶时，接受水（W）或 10%果糖溶液（Fr）的后代被分为六组：第13周时，检测血浆胰岛素、巨噬细胞浸润、活化蛋白激酶（AMPK）和雷帕霉素机制靶标（mTOR）磷酸化以及自噬通量标记物。LP/LPBP/Fr的胰岛素水平和胰岛素抵抗自律模型评估（HOMA-IR）值均低于LP/LP/Fr。LP/LPBP/Fr的AMPK磷酸化上调，mTOR磷酸化下调，微管相关蛋白1A/1B-轻链3B-II（LC3B-II）水平升高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Nutrition & Food Research 工程技术-食品科技

CiteScore

8.70

自引率

1.90%

发文量

250

审稿时长

1.7 months

期刊介绍： Molecular Nutrition & Food Research is a primary research journal devoted to health, safety and all aspects of molecular nutrition such as nutritional biochemistry, nutrigenomics and metabolomics aiming to link the information arising from related disciplines: Bioactivity: Nutritional and medical effects of food constituents including bioavailability and kinetics. Immunology: Understanding the interactions of food and the immune system. Microbiology: Food spoilage, food pathogens, chemical and physical approaches of fermented foods and novel microbial processes. Chemistry: Isolation and analysis of bioactive food ingredients while considering environmental aspects.