Julia Pimentel Arantes, Izadora Dillis Faccin, Luana Rossato, Eduardo João Coutinho, Rafael Carlos Guadagnin, Shaline Sefara Lopes Fernandes, Euclésio Simionatto, Simone Simionatto
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引用次数: 0
Abstract
Essential oils (EOs) are a complex mix of chemical compounds and can have antibacterial activity. Thus, they can improve the delivery and effectiveness of antimicrobials and are promising candidates for the development of antimicrobial alternatives against resistant microorganisms. In this study, we investigated the chemical composition of Cinnamomum triplinerve essential oil (CtEO), as well as the in vitro and in vivo antimicrobial activity alone and in combination with amikacin (AMK). The results of gas chromatography and mass spectrometry (GCMS) analyses showed that the main components of CtEO were (E) – nerolidol (28.0 %) (the major compound), β–selinene (13.6 %), β–elemene (12.0 %), α–selinene (10.9 %), viridiflorol (6.4 %), (E)-caryophyllene (6.3 %), and spathulenol (4.8 %). In vitro, CtEO along with amikacin (AMK) showed synergistic activity against a polymyxin-resistant K. pneumoniae strain. CtEO + AMK can extravasate proteins and nucleic acids within 3 h of treatment. Effective concentrations showed hemocompatibility, no signs of toxicity in vivo in Caenorhabditis elegans and ensured the survival of infected nematodes. Our results suggested that the association of CtEO and AMK exhibited antimicrobial activity against K. pneumoniae; thus, it is a promising therapeutic alternative as an antimicrobial agent.
期刊介绍:
Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.