Effects of Mono- and Multicomponent Nonaqueous-Phase Liquid on the Migration and Retention of Pollutant-degrading Bacteria in Porous Media

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL Water Research Pub Date : 2024-10-20 DOI:10.1016/j.watres.2024.122673

Lei He, Ouyang Li, Jianmei Qin, Chunmao Chen, Zhenshan Li, Meiping Tong

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Abstract

The successful implementation of in-situ bioremediation of nonaqueous-phase liquid (NAPL) contamination in soil-groundwater systems is greatly influenced by the migration performance of NAPL-degrading bacteria. However, the impact and mechanisms of NAPL on the migration/retention of pollutant-degrading bacteria remain unclear. This study investigated the migration/retention performance of A. lwoffii U1091, a strain capable of degrading diesel while producing surfactants, in porous media without and with the presence of mono- and multicomponent NAPL (n-dodecane and diesel) under environmentally relevant conditions. The results showed that under all examined conditions (5 and 50 mM NaCl solution at flow rates of 4 and 8 m/d), the presence of n-dodecane/diesel in porous media could reduce the migration and enhance retention of A. lwoffii in quartz sand columns. Moreover, comparing with mutlicomponent NAPLs of n-dodecane, the monocomponent NAPLs (diesel) exhibited a greater reduction effect on the retention of A. lwoffii in porous media. Through systemically investigating the potential mechanisms via tracer experiment, visible chamber experiment, and theoretical calculation, we found that the reduction in porosity, repulsive forces and movement speeds, the presence of stagnant flow zones in porous media, particularly the biosurfactants generated by A. lwoffii contributed to the enhanced retention of bacteria in NAPL-contaminated porous media. Moreover, owing to presence of the greater amount of hydrophilic components in diesel than in n-dodecane, the available binding sites for the adsorption of bacteria were lower in diesel, resulting in the slightly decreased retention of A. lwoffii in porous media containing diesel than n-dodecane. This study demonstrated that comparing with porous media without NAPL contamination, the retention of strain capable of degrading NAPL in porous media with NAPL contamination was enhanced, beneficial for the subsequent biodegradation of NAPL.

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单组分和多组分非水相液体对多孔介质中污染物降解细菌迁移和滞留的影响

对土壤-地下水系统中的非水相液体（NAPL）污染成功实施原位生物修复，在很大程度上受到 NAPL 降解细菌迁移性能的影响。然而，NAPL 对污染物降解细菌迁移/滞留的影响和机制仍不清楚。本研究调查了 A. lwoffii U1091（一种能够降解柴油并产生表面活性剂的菌株）在没有和存在单组分和多组分 NAPL（正十二烷和柴油）的环境相关条件下，在多孔介质中的迁移/滞留性能。结果表明，在所有考察条件下（5 毫摩尔和 50 毫摩尔氯化钠溶液，流速分别为 4 米/天和 8 米/天），多孔介质中存在正十二烷/柴油可减少 A. lwoffii 在石英砂柱中的迁移并提高其保留率。此外，与正十二烷的多组分 NAPLs 相比，单组分 NAPLs（柴油）对 A. lwoffii 在多孔介质中的滞留有更大的降低作用。通过示踪实验、可见光室实验和理论计算系统地研究了潜在的机理，我们发现孔隙率的降低、排斥力和运动速度的降低、多孔介质中停滞流区的存在，特别是 A. lwoffii 产生的生物表面活性剂促进了细菌在被 NAPL 污染的多孔介质中的滞留。此外，由于柴油中亲水成分的含量高于正十二烷，因此柴油中可用于吸附细菌的结合位点较少，导致 A. lwoffii 在含柴油的多孔介质中的滞留率略低于正十二烷。这项研究表明，与没有受到 NAPL 污染的多孔介质相比，能够降解 NAPL 的菌株在受到 NAPL 污染的多孔介质中的保留率有所提高，有利于随后对 NAPL 的生物降解。

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.