Improving the Stability, Water Solubility, and Antioxidant Activity of α-Tocopherol by Encapsulating It into Niosomes Modified with Cationic Carbamate-Containing Surfactants
Rushana Kushnazarova, Alla Mirgorodskaya, Kseniya Bushmeleva, Alexandra Vyshtakalyuk, Oksana Lenina, Konstantin Petrov, Lucia Zakharova
{"title":"Improving the Stability, Water Solubility, and Antioxidant Activity of α-Tocopherol by Encapsulating It into Niosomes Modified with Cationic Carbamate-Containing Surfactants","authors":"Rushana Kushnazarova, Alla Mirgorodskaya, Kseniya Bushmeleva, Alexandra Vyshtakalyuk, Oksana Lenina, Konstantin Petrov, Lucia Zakharova","doi":"10.1021/acs.langmuir.4c02507","DOIUrl":null,"url":null,"abstract":"The low solubility of α-tocopherol in water and its susceptibility to photodegradation make it difficult for biological systems to absorb this natural antioxidant. To overcome these limitations, α-tocopherol was encapsulated in low-toxicity nanocontainers, namely, niosomes based on Tween 80 and cholesterol. The niosomes were modified with cationic surfactants containing a carbamate fragment. The size and charge of the particles were determined and their stability was assessed using dynamic and electrophoretic light scattering methods. It was found that the introduction of cationic surfactants to niosome formulations significantly improved their physicochemical properties and increased stability due to a positive charge of up to +40 mV being generated. Modified niosomes loaded with α-tocopherol were characterized by a hydrodynamic diameter of 100–120 nm, a narrow particle size distribution, and a high encapsulation efficiency of more than 90%. Testing the photochemical stability of α-tocopherol using a spectrophotometric method demonstrated that niosomes were able to protect this substance from UV irradiation. Luminescence analysis showed that the inclusion of α-tocopherol in niosomes increased their antioxidant activity by 30%. An acute toxicity study has demonstrated the safety of the systems. The LD<sub>50</sub> value for niosomes modified with carbamate-containing surfactants and loaded with α-tocopherol exceeded 10,000 mg·kg<sup>–1</sup> (mice, intraperitoneal and oral administration).","PeriodicalId":50,"journal":{"name":"Langmuir","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Langmuir","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.langmuir.4c02507","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
The low solubility of α-tocopherol in water and its susceptibility to photodegradation make it difficult for biological systems to absorb this natural antioxidant. To overcome these limitations, α-tocopherol was encapsulated in low-toxicity nanocontainers, namely, niosomes based on Tween 80 and cholesterol. The niosomes were modified with cationic surfactants containing a carbamate fragment. The size and charge of the particles were determined and their stability was assessed using dynamic and electrophoretic light scattering methods. It was found that the introduction of cationic surfactants to niosome formulations significantly improved their physicochemical properties and increased stability due to a positive charge of up to +40 mV being generated. Modified niosomes loaded with α-tocopherol were characterized by a hydrodynamic diameter of 100–120 nm, a narrow particle size distribution, and a high encapsulation efficiency of more than 90%. Testing the photochemical stability of α-tocopherol using a spectrophotometric method demonstrated that niosomes were able to protect this substance from UV irradiation. Luminescence analysis showed that the inclusion of α-tocopherol in niosomes increased their antioxidant activity by 30%. An acute toxicity study has demonstrated the safety of the systems. The LD50 value for niosomes modified with carbamate-containing surfactants and loaded with α-tocopherol exceeded 10,000 mg·kg–1 (mice, intraperitoneal and oral administration).
期刊介绍:
Langmuir is an interdisciplinary journal publishing articles in the following subject categories:
Colloids: surfactants and self-assembly, dispersions, emulsions, foams
Interfaces: adsorption, reactions, films, forces
Biological Interfaces: biocolloids, biomolecular and biomimetic materials
Materials: nano- and mesostructured materials, polymers, gels, liquid crystals
Electrochemistry: interfacial charge transfer, charge transport, electrocatalysis, electrokinetic phenomena, bioelectrochemistry
Devices and Applications: sensors, fluidics, patterning, catalysis, photonic crystals
However, when high-impact, original work is submitted that does not fit within the above categories, decisions to accept or decline such papers will be based on one criteria: What Would Irving Do?
Langmuir ranks #2 in citations out of 136 journals in the category of Physical Chemistry with 113,157 total citations. The journal received an Impact Factor of 4.384*.
This journal is also indexed in the categories of Materials Science (ranked #1) and Multidisciplinary Chemistry (ranked #5).