Wenqian Liao, Xuewei Huang, Zhi-Long Ye, Tianyi Zhang, Jiasheng Cai, Yahui Huang, Yanlin Li
{"title":"Insights into the migration mechanism of extracellular antibiotic resistance genes during struvite recovery using synthetic wastewater","authors":"Wenqian Liao, Xuewei Huang, Zhi-Long Ye, Tianyi Zhang, Jiasheng Cai, Yahui Huang, Yanlin Li","doi":"10.1016/j.watres.2024.122681","DOIUrl":null,"url":null,"abstract":"In recent, the complexation of extracellular antibiotic resistance genes (eARGs) with environmental particles has been getting significant concerns, since eARGs can consequently disseminate, propagate and pose ecological risks to the environment. This study focused on eARGs complexing with struvite (MgNH<sub>4</sub>PO<sub>4</sub>·6H<sub>2</sub>O) particles in struvite recovery by using synthetic wastewater. The adsorption capacities of eARGs by struvite crystals with different morphologies were firstly examined. Results revealed that struvite crystals possessed the maximum eARGs adsorption capacity of 7.95 × 10<sup>12</sup>-1.76 × 10<sup>13</sup> copies/g. The evolution of struvite morphologies from regular polyhedron to needle-like, coupled with larger BET surface area, resulted in a matching increase relationship of eARGs adsorption. Electrostatic interaction and covalent binding were the predominant forces between eARGs and struvite crystals, attributed to the Mg[H<sub>2</sub>O]<sub>6</sub><sup>2+</sup> octahedra in the struvite crystallite and the phosphate backbone with its external position in eARGs molecule. The eARGs adsorption in struvite crystallization displayed a “U” curve with the minimum values of 3.57 × 10<sup>12</sup>-7.28 × 10<sup>12</sup> copies/g at pH 8.8, which was ascribed to the excessive existence of Mg<sup>2+</sup> ions in the liquid. Despite the gradual increase in the Mg:P molar ratio from 1.0 to 2.5 during crystallization, the abundance of eARGs on recovered solids displayed twice dramatic declines with two or three orders of magnitude lower, which was attributed to the formation and binding saturation of eARGs-Mg chelate, as well as the non-negligible evolution of magnesium species under different pH values. These outcomes provide new insights into the migration behavior of eARGs during struvite recovery from wastewater.","PeriodicalId":443,"journal":{"name":"Water Research","volume":null,"pages":null},"PeriodicalIF":11.4000,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Water Research","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.watres.2024.122681","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
引用次数: 0
Abstract
In recent, the complexation of extracellular antibiotic resistance genes (eARGs) with environmental particles has been getting significant concerns, since eARGs can consequently disseminate, propagate and pose ecological risks to the environment. This study focused on eARGs complexing with struvite (MgNH4PO4·6H2O) particles in struvite recovery by using synthetic wastewater. The adsorption capacities of eARGs by struvite crystals with different morphologies were firstly examined. Results revealed that struvite crystals possessed the maximum eARGs adsorption capacity of 7.95 × 1012-1.76 × 1013 copies/g. The evolution of struvite morphologies from regular polyhedron to needle-like, coupled with larger BET surface area, resulted in a matching increase relationship of eARGs adsorption. Electrostatic interaction and covalent binding were the predominant forces between eARGs and struvite crystals, attributed to the Mg[H2O]62+ octahedra in the struvite crystallite and the phosphate backbone with its external position in eARGs molecule. The eARGs adsorption in struvite crystallization displayed a “U” curve with the minimum values of 3.57 × 1012-7.28 × 1012 copies/g at pH 8.8, which was ascribed to the excessive existence of Mg2+ ions in the liquid. Despite the gradual increase in the Mg:P molar ratio from 1.0 to 2.5 during crystallization, the abundance of eARGs on recovered solids displayed twice dramatic declines with two or three orders of magnitude lower, which was attributed to the formation and binding saturation of eARGs-Mg chelate, as well as the non-negligible evolution of magnesium species under different pH values. These outcomes provide new insights into the migration behavior of eARGs during struvite recovery from wastewater.
期刊介绍:
Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include:
•Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management;
•Urban hydrology including sewer systems, stormwater management, and green infrastructure;
•Drinking water treatment and distribution;
•Potable and non-potable water reuse;
•Sanitation, public health, and risk assessment;
•Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions;
•Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment;
•Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution;
•Environmental restoration, linked to surface water, groundwater and groundwater remediation;
•Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts;
•Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle;
•Socio-economic, policy, and regulations studies.