Identification and Characterization of Innate Immunity in Actinidia melanandra in Response to Pseudomonas syringae pv. actinidiae.

IF 6 1区 生物学 Q1 PLANT SCIENCES Plant, Cell & Environment Pub Date : 2024-10-13 DOI:10.1111/pce.15189
Lauren M Hemara, Abhishek Chatterjee, Shin-Mei Yeh, Ronan K Y Chen, Elena Hilario, Liam Le Lievre, Ross N Crowhurst, Deborah Bohne, Saadiah Arshed, Haileigh R Patterson, Kelvina Barrett-Manako, Susan Thomson, Andrew C Allan, Cyril Brendolise, David Chagné, Matthew D Templeton, Jibran Tahir, Jay Jayaraman
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Abstract

Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) has decimated kiwifruit orchards growing susceptible kiwifruit Actinidia chinensis varieties. Effector loss has occurred recently in Psa3 isolates from resistant kiwifruit germplasm, resulting in strains capable of partially overcoming resistance present in kiwiberry vines (Actinidia arguta, Actinidia polygama, and Actinidia melanandra). Diploid male A. melanandra recognises several effectors, sharing recognition of at least one avirulence effector (HopAW1a) with previously studied tetraploid kiwiberry vines. Sequencing and assembly of the A. melanandra genome enabled the characterisation of the transcriptomic response of this non-host to wild-type and genetic mutants of Psa3. A. melanandra appears to mount a classic effector-triggered immunity (ETI) response to wildtype Psa3 V-13, as expected. Surprisingly, the type III secretion (T3SS) system-lacking Psa3 V-13 ∆hrcC strain did not appear to trigger pattern-triggered immunity (PTI) despite lacking the ability to deliver immunity-suppressing effectors. Contrasting the A. melanandra responses to an effectorless Psa3 V-13 ∆33E strain and to Psa3 V-13 ∆hrcC suggested that PTI triggered by Psa3 V-13 was based on the recognition of the T3SS itself. The characterisation of both ETI and PTI branches of innate immunity responses within A. melanandra further enables breeding for durable resistance in future kiwifruit cultivars.

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鉴定和描述黑色放线菌对 Pseudomonas syringae pv. actinidiae 的先天免疫反应。
Pseudomonas syringae pv. actinidiae biovar 3(Psa3)已使种植易感猕猴桃 Actinidia chinensis 品种的猕猴桃果园遭受灭顶之灾。最近,从抗性猕猴桃种质中分离出的 Psa3 株系中出现了效应器缺失,从而产生了能够部分克服猕猴桃藤蔓(Actinidia arguta、Actinidia polygama 和 Actinidia melanandra)抗性的株系。二倍体雄性 A. melanandra 能识别多种效应物,与之前研究过的四倍体猕猴桃藤共同识别至少一种抗性效应物(HopAW1a)。通过对 A. melanandra 基因组的测序和组装,可以确定这种非宿主对 Psa3 野生型和遗传突变体的转录组反应的特征。正如预期的那样,A. melanandra似乎对野生型Psa3 V-13做出了典型的效应触发免疫(ETI)反应。令人惊讶的是,缺乏 III 型分泌(T3SS)系统的 Psa3 V-13 ∆hrcC 菌株尽管缺乏传递免疫抑制效应物的能力,但似乎并没有触发模式触发免疫(PTI)。对比黑腹角雉对无效应物的 Psa3 V-13 ∆33E 株系和 Psa3 V-13 ∆hrcC 株系的反应,发现 Psa3 V-13 触发的 PTI 是基于对 T3SS 本身的识别。对 A. melanandra 体内先天性免疫反应的 ETI 和 PTI 两个分支进行鉴定,有助于进一步培育未来猕猴桃栽培品种的持久抗性。
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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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