Enhanced SERS detection of the colorectal cancer biomarker utilizing a two-dimensional silver substrate.

IF 5.4 2区医学 Q1 BIOPHYSICS Colloids and Surfaces B: Biointerfaces Pub Date : 2024-10-14 DOI:10.1016/j.colsurfb.2024.114317

Qingling Nie, Peipei Xu, Jiajia Liu, Yanhanzhuo Wang, Qian Huang, Ruiyun You, Yunzhen Liu, Lizhi Li, Yudong Lu

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Abstract

To improve the sensitivity, accuracy and specificity of the assay, a two-dimensional silver substrate with EF=5.85×10⁸ was first synthesized as a SERS substrate, on the surface of which DSP molecules were modified to form a DSP-antibody coupling through the activation of two N-hydroxy succinimide (NHS) esters to capture TNF-α. Subsequently, aptamerized silver-coated gold nanospheres (Au@TFMBA@Ag) were synthesized as Surface-Enhanced Raman Scattering (SERS) recognition probes. These probes were employed to create a sandwich structure for the quantitative detection of Tumor Necrosis Factor-alpha (TNF-α), utilizing the SERS signal intensity at 1374 cm^-1. Quantitative detection of TNF-α was successfully accomplished within the concentration range of 10^-4 to 10^-10 mg·mL^-1. Clinical serum samples were collected and subjected to testing. Significance analysis, conducted through the T-test (p < 0.0001), unequivocally showed the method's ability to differentiate between sera from normal individuals and those diagnosed with colon cancer.

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利用二维银基底增强对结直肠癌生物标记物的 SERS 检测。

为了提高检测的灵敏度、准确性和特异性，首先合成了EF=5.85×108的二维银基底作为SERS基底，在其表面修饰DSP分子，通过活化两个N-羟基琥珀酰亚胺（NHS）酯形成DSP-抗体偶联，以捕获TNF-α。随后，合成了银涂层金纳米球（Au@TFMBA@Ag）作为表面增强拉曼散射（SERS）识别探针。利用这些探针在 1374 cm-1 处的 SERS 信号强度创建了一种夹层结构，用于定量检测肿瘤坏死因子-α（TNF-α）。在 10-4 至 10-10 mg-mL-1 的浓度范围内，成功实现了 TNF-α 的定量检测。采集临床血清样本并进行检测。通过 T 检验（P < 0.0001）进行的显著性分析明确显示，该方法能够区分正常人和结肠癌患者的血清。

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.