Red blood cell (RBC)-based carriers have emerged as promising vehicles for drug delivery due to their inherent biocompatibility and biodegradability. Traditional methods for loading nanoparticles (NPs) onto RBC surfaces often involve labor-intensive processes like incubation and multiple centrifugation steps, limiting their practicality and controllability. In this study, we introduce a fully integrated acoustofluidic platform that enables one-step preparation of NP-loaded RBC carriers with controlled modification and on-site purification. By incorporating a high-frequency bulk acoustic wave (BAW) resonator into a microfluidic chip, we utilize acoustic streaming effects to manipulate the movement and interaction of RBCs and NPs within the microchannel. This design allows for precise control over NP loading efficiency by adjusting the input power to the resonator. Experimental results using 200 nm positively charged fluorescent NPs demonstrate that our platform significantly enhances the interaction between RBCs and NPs, achieving efficient and controllable surface loading of NPs onto RBCs. Furthermore, the platform simplifies post-processing by directing excess NPs to waste outlets, eliminating the need for repetitive washing and centrifugation. This acoustofluidics approach not only automates the loading process but also offers high controllability, highlighting its potential for various applications in particle and cell surface modification.
{"title":"One-step on-chip preparation of nanoparticle-conjugated red blood cell carriers","authors":"Huihui Xu , Rui You , Huijing Zhang, Wei Wei, Tiechuan Li, Xuexin Duan","doi":"10.1016/j.colsurfb.2024.114373","DOIUrl":"10.1016/j.colsurfb.2024.114373","url":null,"abstract":"<div><div>Red blood cell (RBC)-based carriers have emerged as promising vehicles for drug delivery due to their inherent biocompatibility and biodegradability. Traditional methods for loading nanoparticles (NPs) onto RBC surfaces often involve labor-intensive processes like incubation and multiple centrifugation steps, limiting their practicality and controllability. In this study, we introduce a fully integrated acoustofluidic platform that enables one-step preparation of NP-loaded RBC carriers with controlled modification and on-site purification. By incorporating a high-frequency bulk acoustic wave (BAW) resonator into a microfluidic chip, we utilize acoustic streaming effects to manipulate the movement and interaction of RBCs and NPs within the microchannel. This design allows for precise control over NP loading efficiency by adjusting the input power to the resonator. Experimental results using 200 nm positively charged fluorescent NPs demonstrate that our platform significantly enhances the interaction between RBCs and NPs, achieving efficient and controllable surface loading of NPs onto RBCs. Furthermore, the platform simplifies post-processing by directing excess NPs to waste outlets, eliminating the need for repetitive washing and centrifugation. This acoustofluidics approach not only automates the loading process but also offers high controllability, highlighting its potential for various applications in particle and cell surface modification.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"246 ","pages":"Article 114373"},"PeriodicalIF":5.4,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142651000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-13DOI: 10.1016/j.colsurfb.2024.114374
Junjie Zhang , Li Lu , Wenqing Zhang , Yuchen Miao , Hengda Du , Hui Xia , Zhiyong Tao , Zhaofeng Du , Yulong Tang , Qiang Fang
Triple-negative breast cancer (TNBC) is a highly aggressive subtype with limited therapeutic options, often exhibiting resistance to standard radiotherapy (RT) and chemotherapy. Recent advancements in nanomedicine provide an opportunity to enhance treatment efficacy through innovative drug delivery systems and radiosensitizers. In this study, we present a novel nanotheranostic platform, MOs-G@DOX, engineered to enhance the therapeutic efficacy of RT in the treatment of TNBC. This platform consists of gadolinium-containing mesoporous organosilica nanoparticles (MOs-G) that serve a dual function as a drug carrier and a radiosensitizer. The MOs-G were synthesized via a surfactant-mediated sol-gel process, followed by gadolinium incorporation through nanoprecipitation. The antitumor drug doxorubicin (DOX) was subsequently loaded into the mesoporous structure, forming the MOs-G@DOX nanoplatform. Comprehensive in vitro and in vivo studies demonstrated that MOs-G@DOX exhibits excellent biocompatibility and significantly enhances the radiosensitivity of TNBC cells, leading to superior tumor growth inhibition compared to conventional treatments. The stability of MOs-G, with minimal gadolinium ion leakage, further underscores its potential as a safe and effective nanomedicine. Additionally, the combination of MOs-G@DOX with RT showed a marked increase in reactive oxygen species (ROS) generation and tumor cell apoptosis, which were confirmed through histological analyses. These findings suggest that MOs-G@DOX is a promising candidate for advancing cancer therapy, particularly in the context of RT for TNBC.
{"title":"Gadolinium ion-loaded mesoporous organosilica nanoplatform for enhanced radiotherapy in breast tumor treatment","authors":"Junjie Zhang , Li Lu , Wenqing Zhang , Yuchen Miao , Hengda Du , Hui Xia , Zhiyong Tao , Zhaofeng Du , Yulong Tang , Qiang Fang","doi":"10.1016/j.colsurfb.2024.114374","DOIUrl":"10.1016/j.colsurfb.2024.114374","url":null,"abstract":"<div><div>Triple-negative breast cancer (TNBC) is a highly aggressive subtype with limited therapeutic options, often exhibiting resistance to standard radiotherapy (RT) and chemotherapy. Recent advancements in nanomedicine provide an opportunity to enhance treatment efficacy through innovative drug delivery systems and radiosensitizers. In this study, we present a novel nanotheranostic platform, MOs-G@DOX, engineered to enhance the therapeutic efficacy of RT in the treatment of TNBC. This platform consists of gadolinium-containing mesoporous organosilica nanoparticles (MOs-G) that serve a dual function as a drug carrier and a radiosensitizer. The MOs-G were synthesized <em>via</em> a surfactant-mediated sol-gel process, followed by gadolinium incorporation through nanoprecipitation. The antitumor drug doxorubicin (DOX) was subsequently loaded into the mesoporous structure, forming the MOs-G@DOX nanoplatform. Comprehensive <em>in vitro</em> and <em>in vivo</em> studies demonstrated that MOs-G@DOX exhibits excellent biocompatibility and significantly enhances the radiosensitivity of TNBC cells, leading to superior tumor growth inhibition compared to conventional treatments. The stability of MOs-G, with minimal gadolinium ion leakage, further underscores its potential as a safe and effective nanomedicine. Additionally, the combination of MOs-G@DOX with RT showed a marked increase in reactive oxygen species (ROS) generation and tumor cell apoptosis, which were confirmed through histological analyses. These findings suggest that MOs-G@DOX is a promising candidate for advancing cancer therapy, particularly in the context of RT for TNBC.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"246 ","pages":"Article 114374"},"PeriodicalIF":5.4,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-13DOI: 10.1016/j.colsurfb.2024.114376
Xiaodan Su , Huashuai Zhong , Yongzhu Zeng , Yuyan Zhang , Bo Zhang , Wei Guo , Qiujie Huang , Yong Ye
Background
Liver fibrosis is a common stage of various chronic liver diseases, often developing into liver cirrhosis, and even liver cancer. Activated hepatic stellate cells (aHSCs) have been shown to promote the development of liver fibrosis. Therefore, dual-targeted combination therapy for liver may be an effective strategy for the treatment of liver fibrosis.
Purpose
In this study, the novel nanostructured lipid carriers (GA&GalNH2-DC-NLCs) were prepared for Dehydrocavidine (DC), glycyrrhetinic acid (GA) and galactose-PEG2000-NH2 (GalNH2) were selected as targeted ligand-modified nanostructured lipid carriers (NLCs), which enables dual-targeting to the liver for the treatment of liver fibrosis.
Study design
To study the targeting effect of GA&GalNH2-DC-NLCs on liver and its therapeutic effect on liver fibrosis, we established aHSC-T6 cell model and rat model of liver fibrosis for study.
Results
GA&GalNH2-DC-NLCs promoted drug liver targeting efficiency and apoptosis rate by upregulating the expression of Bax. It showed that compared with no and/or GA-modified NLCs and GalNH2-modified NLCs, GA&GalNH2-DC-NLCs exhibited less extracellular matrix (ECM) deposition, induced apoptosis of aHSCs, and stronger anti-fibrosis effects in vivo. This may be due the fact that GA or GalNH2-modifified NLCs simultaneously block HSCs activation and inhibit the IL-6/STAT3 pathway.
Conclusion
GA&GalNH2-DC-NLCs is thus a potential strategy for liver fibrosis treatment.
{"title":"Dual-ligand-functionalized nanostructured lipid carriers as a novel dehydrocavidine delivery system for liver fibrosis therapy","authors":"Xiaodan Su , Huashuai Zhong , Yongzhu Zeng , Yuyan Zhang , Bo Zhang , Wei Guo , Qiujie Huang , Yong Ye","doi":"10.1016/j.colsurfb.2024.114376","DOIUrl":"10.1016/j.colsurfb.2024.114376","url":null,"abstract":"<div><h3>Background</h3><div>Liver fibrosis is a common stage of various chronic liver diseases, often developing into liver cirrhosis, and even liver cancer. Activated hepatic stellate cells (aHSCs) have been shown to promote the development of liver fibrosis. Therefore, dual-targeted combination therapy for liver may be an effective strategy for the treatment of liver fibrosis.</div></div><div><h3>Purpose</h3><div>In this study, the novel nanostructured lipid carriers (GA&GalNH<sub>2</sub>-DC-NLCs) were prepared for Dehydrocavidine (DC), glycyrrhetinic acid (GA) and galactose-PEG<sub>2000</sub>-NH<sub>2</sub> (GalNH<sub>2</sub>) were selected as targeted ligand-modified nanostructured lipid carriers (NLCs), which enables dual-targeting to the liver for the treatment of liver fibrosis.</div></div><div><h3>Study design</h3><div>To study the targeting effect of GA&GalNH<sub>2</sub>-DC-NLCs on liver and its therapeutic effect on liver fibrosis, we established aHSC-T6 cell model and rat model of liver fibrosis for study.</div></div><div><h3>Results</h3><div>GA&GalNH<sub>2</sub>-DC-NLCs promoted drug liver targeting efficiency and apoptosis rate by upregulating the expression of Bax. It showed that compared with no and/or GA-modified NLCs and GalNH<sub>2</sub>-modified NLCs, GA&GalNH<sub>2</sub>-DC-NLCs exhibited less extracellular matrix (ECM) deposition, induced apoptosis of aHSCs, and stronger anti-fibrosis effects in vivo. This may be due the fact that GA or GalNH<sub>2</sub>-modifified NLCs simultaneously block HSCs activation and inhibit the IL-6/STAT3 pathway.</div></div><div><h3>Conclusion</h3><div>GA&GalNH<sub>2</sub>-DC-NLCs is thus a potential strategy for liver fibrosis treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"246 ","pages":"Article 114376"},"PeriodicalIF":5.4,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-12DOI: 10.1016/j.colsurfb.2024.114375
Chang Li , Xiaocong Li , Xinping Liu , Li Yuan , Xiao Duan , Wei Guo
Hydrogels have been widely used for in situ postoperative chemotherapy due to fewer side effects and longer duration of drug action compared to systemic chemotherapy. This paper reviews the application of natural materials-based hydrogels after tumor resection to explore them as an option for postoperative chemotherapy. Different material-based hydrogels, different response-based hydrogels, and the clinical applications of implantable, injectable, and sprayed hydrogels were investigated and summarized. Based on the main content, we report the possible clinical application prospects and typical functions of hydrogel-based local drug delivery systems.
{"title":"Recent progress of natural materials-based hydrogel for postoperative chemotherapy","authors":"Chang Li , Xiaocong Li , Xinping Liu , Li Yuan , Xiao Duan , Wei Guo","doi":"10.1016/j.colsurfb.2024.114375","DOIUrl":"10.1016/j.colsurfb.2024.114375","url":null,"abstract":"<div><div>Hydrogels have been widely used for <em>in situ</em> postoperative chemotherapy due to fewer side effects and longer duration of drug action compared to systemic chemotherapy. This paper reviews the application of natural materials-based hydrogels after tumor resection to explore them as an option for postoperative chemotherapy. Different material-based hydrogels, different response-based hydrogels, and the clinical applications of implantable, injectable, and sprayed hydrogels were investigated and summarized. Based on the main content, we report the possible clinical application prospects and typical functions of hydrogel-based local drug delivery systems.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"246 ","pages":"Article 114375"},"PeriodicalIF":5.4,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-12DOI: 10.1016/j.colsurfb.2024.114380
Xu Shao , Muqiong Li , Chaoren Yan , Chao Wang , Xin Wang , Ping Guan , Xiaoling Hu , Li Fan
Alzheimer’s disease (AD) is associated with amyloid production and buildup in the brain, leading to neurodegeneration. In this study, we used a solvent-thermal technique to produce light-sensitive carbon nanodots (L-CNDs). L-CNDs exhibit outstanding photocatalytic properties, producing singlet oxygen (1O2) under 630 nm irradiation. L-CNDs have a high photothermal conversion efficiency of 68.25 % under 808 nm irradiation, allowing for localized heating and regulation of Aβ aggregation. L-CNDs bind Aβ through hydrophobic interaction and π-π stacking. L-CNDs inhibit Aβ aggregation with efficiencies of 61.08 %, 75.09 %, and 91.72 % at 10 μg·mL−1 in photothermal therapy (PTT), photodynamic therapy (PDT), and PTT/PDT synergistic therapy, respectively. L-CNDs efficiently suppress Aβ misfolding, inhibit fibrillation, and promote disaggregation of mature fibrils. L-CNDs mitigate Aβ-induced cytotoxicity in PC12 and HT22 cells. Interestingly, the data showed that 84.6 % of the L-CNDs could penetrate bEnd.3 cells after 8 h of treatment, demonstrating that they have the capacity to cross the blood-brain barrier (BBB) because of their small size. In vitro investigations have shown that L-CNDs can pass through the BBB. Collectively, our findings reveal a unique technique for treating amyloid disorders using carbon nanodots with significant potential for future studies in this area.
{"title":"Photocatalytic, photothermal, and blood-brain barrier-permeable carbon nanodots: A potent multifunctional scavenger for β-amyloid plaque","authors":"Xu Shao , Muqiong Li , Chaoren Yan , Chao Wang , Xin Wang , Ping Guan , Xiaoling Hu , Li Fan","doi":"10.1016/j.colsurfb.2024.114380","DOIUrl":"10.1016/j.colsurfb.2024.114380","url":null,"abstract":"<div><div>Alzheimer’s disease (AD) is associated with amyloid production and buildup in the brain, leading to neurodegeneration. In this study, we used a solvent-thermal technique to produce light-sensitive carbon nanodots (L-CNDs). L-CNDs exhibit outstanding photocatalytic properties, producing singlet oxygen (<sup>1</sup>O<sub>2</sub>) under 630 nm irradiation. L-CNDs have a high photothermal conversion efficiency of 68.25 % under 808 nm irradiation, allowing for localized heating and regulation of A<em>β</em> aggregation. L-CNDs bind A<em>β</em> through hydrophobic interaction and π-π stacking. L-CNDs inhibit A<em>β</em> aggregation with efficiencies of 61.08 %, 75.09 %, and 91.72 % at 10 μg·mL<sup>−1</sup> in photothermal therapy (PTT), photodynamic therapy (PDT), and PTT/PDT synergistic therapy, respectively. L-CNDs efficiently suppress A<em>β</em> misfolding, inhibit fibrillation, and promote disaggregation of mature fibrils. L-CNDs mitigate A<em>β</em>-induced cytotoxicity in PC12 and HT22 cells. Interestingly, the data showed that 84.6 % of the L-CNDs could penetrate bEnd.3 cells after 8 h of treatment, demonstrating that they have the capacity to cross the blood-brain barrier (BBB) because of their small size. <em>In vitro</em> investigations have shown that L-CNDs can pass through the BBB. Collectively, our findings reveal a unique technique for treating amyloid disorders using carbon nanodots with significant potential for future studies in this area.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"246 ","pages":"Article 114380"},"PeriodicalIF":5.4,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-12DOI: 10.1016/j.colsurfb.2024.114378
Meng Zhong , Hongwei He , Liaochuan Wang , Linyun Pu , Zhirong Liu , Jiangtian Wang , Yanjun Zhang , Shiliang Wang , Bingbing Sun , Xiaojing Li , Lidan Xiong , Jiabo Mi , Mowei Song , Panxianzhi Ni , Can Huang , Tun Yuan , Jie Liang , Yujiang Fan , Xingdong Zhang
Skin injection filling products must undergo a rigorous evaluation before entering the market, and intradermal reactions are crucial for biocompatibility. However, current evaluation methods for skin injection fillers often lack precision. This study aims to explore a comprehensive evaluation method by incorporating additional indicators: dermoscopy, transepidermal water loss, moisture content, ultrasound, and histological observation. Experimental findings show sodium hyaluronate gel groups exhibited no noticeable erythema or abnormalities, except for skin swelling. The dynamic and quantitative assessment of water content and transdermal water loss provided insights into the injection site skin's edema status. Ultrasound imaging observed subcutaneous material occupation and measured skin protrusion height. Histopathological observations revealed edema and inflammation in positive groups but no significant reactions in any gel samples. The comprehensive use of these methods confirms that the intradermal response of injection fillers in this study is within an acceptable range. However, if solely evaluated based on existing standards, it may exceed the qualified range. Consequently, the study suggests that for sodium hyaluronate gel, a macromolecular injection filler for long-term subcutaneous space occupancy, the traditional intradermal reaction scoring method may be inadequate. The proposed comprehensive evaluation scheme in this study is considered a more objective approach.
{"title":"Comprehensive assessment of intradermal responses to hyaluronic acid-based skin injection fillers through multi-pathway dynamic synergies","authors":"Meng Zhong , Hongwei He , Liaochuan Wang , Linyun Pu , Zhirong Liu , Jiangtian Wang , Yanjun Zhang , Shiliang Wang , Bingbing Sun , Xiaojing Li , Lidan Xiong , Jiabo Mi , Mowei Song , Panxianzhi Ni , Can Huang , Tun Yuan , Jie Liang , Yujiang Fan , Xingdong Zhang","doi":"10.1016/j.colsurfb.2024.114378","DOIUrl":"10.1016/j.colsurfb.2024.114378","url":null,"abstract":"<div><div>Skin injection filling products must undergo a rigorous evaluation before entering the market, and intradermal reactions are crucial for biocompatibility. However, current evaluation methods for skin injection fillers often lack precision. This study aims to explore a comprehensive evaluation method by incorporating additional indicators: dermoscopy, transepidermal water loss, moisture content, ultrasound, and histological observation. Experimental findings show sodium hyaluronate gel groups exhibited no noticeable erythema or abnormalities, except for skin swelling. The dynamic and quantitative assessment of water content and transdermal water loss provided insights into the injection site skin's edema status. Ultrasound imaging observed subcutaneous material occupation and measured skin protrusion height. Histopathological observations revealed edema and inflammation in positive groups but no significant reactions in any gel samples. The comprehensive use of these methods confirms that the intradermal response of injection fillers in this study is within an acceptable range. However, if solely evaluated based on existing standards, it may exceed the qualified range. Consequently, the study suggests that for sodium hyaluronate gel, a macromolecular injection filler for long-term subcutaneous space occupancy, the traditional intradermal reaction scoring method may be inadequate. The proposed comprehensive evaluation scheme in this study is considered a more objective approach.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"246 ","pages":"Article 114378"},"PeriodicalIF":5.4,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-09DOI: 10.1016/j.colsurfb.2024.114372
Yuxin Guo , Peipei Wan , Yue Xu , Siqin Zhang , Chenhui Li , Yueying Wang , Weili Heng , Wenjun Miao
Photodynamic therapy (PDT) is an emerging cancer therapy known for its non-invasive approach and minimal side effects. However, the clinical effectiveness of PDT is limited by the poor distribution and penetration of photosensitizers (PS) in tumors. In this research, we developed a novel delivery system for PS, termed EWC, using the facultative probiotic Escherichia coli Nissle 1917 (EcN) as a carrier. Chlorin e6 (Ce6) was electrostatically adsorbed onto the surface of EcN with the assistance of water-soluble chitosan (WCS). EWC demonstrated effective photodynamic activity and was readily internalized by human lung cancer cells (A549). In vitro assays confirmed its low toxicity to mammalian cells and potent photodynamic cytotoxicity against A549 cells. Additionally, EWC penetrated tumor spheroids and inhibited their growth, as shown by 3D fluorescence imaging. In vivo tests revealed that EWC enhanced the distribution and accumulation of Ce6 at the tumor site, effectively inhibiting tumor growth under light stimulation. Moreover, EWC exhibited excellent biocompatibility in mice. This facultative probiotics-based delivery system significantly improves the efficiency of PDT, offering a promising approach for low-toxicity and high-efficiency tumor therapy.
{"title":"Facultative probiotics enable improved tumor distribution and deep penetration of photosensitizer for enhanced photodynamic therapy","authors":"Yuxin Guo , Peipei Wan , Yue Xu , Siqin Zhang , Chenhui Li , Yueying Wang , Weili Heng , Wenjun Miao","doi":"10.1016/j.colsurfb.2024.114372","DOIUrl":"10.1016/j.colsurfb.2024.114372","url":null,"abstract":"<div><div>Photodynamic therapy (PDT) is an emerging cancer therapy known for its non-invasive approach and minimal side effects. However, the clinical effectiveness of PDT is limited by the poor distribution and penetration of photosensitizers (PS) in tumors. In this research, we developed a novel delivery system for PS, termed EWC, using the facultative probiotic <em>Escherichia coli Nissle</em> 1917 (EcN) as a carrier. Chlorin e6 (Ce6) was electrostatically adsorbed onto the surface of EcN with the assistance of water-soluble chitosan (WCS). EWC demonstrated effective photodynamic activity and was readily internalized by human lung cancer cells (A549). <em>In vitro</em> assays confirmed its low toxicity to mammalian cells and potent photodynamic cytotoxicity against A549 cells. Additionally, EWC penetrated tumor spheroids and inhibited their growth, as shown by 3D fluorescence imaging. <em>In vivo</em> tests revealed that EWC enhanced the distribution and accumulation of Ce6 at the tumor site, effectively inhibiting tumor growth under light stimulation. Moreover, EWC exhibited excellent biocompatibility in mice. This facultative probiotics-based delivery system significantly improves the efficiency of PDT, offering a promising approach for low-toxicity and high-efficiency tumor therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"246 ","pages":"Article 114372"},"PeriodicalIF":5.4,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-08DOI: 10.1016/j.colsurfb.2024.114371
Li Qin , Jianfei Tu , Jiawei Zhao , Yuanke Zhang , Tiancheng Li , Yuqi Zhang , Peng Zhang , Guixia Ling , Jiansong Ji
Conventional chemotherapy drugs are difficult to effectively target tumor tissue, leading to poor treatment outcomes and side effects. Actively targeted and stimuli-responsive nanomedicine greatly improves this situation, allowing for more precise drug accumulation at tumor sites. Herein, carboxymethyl-β-cyclodextrin (CMCD) - based host-guest nanocomposites (NPs) encapsulating hydroxycamptothecin (HCPT) were fabricated, which responded to esterase and had the function of targeting CD 44 receptors and the nucleus. PS-CMCD was firstly synthesized through an amide reaction of protamine (PS) and CMCD to enhance the function of penetrating membrane and nuclear localization. PS-CMCD/HCPT/HA NPs were then prepared by the host-guest complexation of PS-CMCD and HCPT and followed by surface modification of hyaluronic acid (HA) with CD44 receptor-targeting properties. The successful inclusion was also validated through computer simulation. The obtained nanocomposites displayed the esterase-responsive release behaviors of HCPT. Moreover, the synthesized PS-CMCD/HCPT/HA NPs enhanced the intracellular drug uptake due to the tumor cell- and nuclear-mediated targeting. In addition, in vivo application exhibited that PS-CMCD/HCPT/HA NPs realized good antitumor effects. These findings suggested its potential for targeted delivery and more effective tumor therapy.
{"title":"Dual-targeted and esterase-responsive cyclodextrin-based host-guest nanocomposites for enhanced antitumor therapy","authors":"Li Qin , Jianfei Tu , Jiawei Zhao , Yuanke Zhang , Tiancheng Li , Yuqi Zhang , Peng Zhang , Guixia Ling , Jiansong Ji","doi":"10.1016/j.colsurfb.2024.114371","DOIUrl":"10.1016/j.colsurfb.2024.114371","url":null,"abstract":"<div><div>Conventional chemotherapy drugs are difficult to effectively target tumor tissue, leading to poor treatment outcomes and side effects. Actively targeted and stimuli-responsive nanomedicine greatly improves this situation, allowing for more precise drug accumulation at tumor sites. Herein, carboxymethyl-β-cyclodextrin (CMCD) - based host-guest nanocomposites (NPs) encapsulating hydroxycamptothecin (HCPT) were fabricated, which responded to esterase and had the function of targeting CD 44 receptors and the nucleus. PS-CMCD was firstly synthesized through an amide reaction of protamine (PS) and CMCD to enhance the function of penetrating membrane and nuclear localization. PS-CMCD/HCPT/HA NPs were then prepared by the host-guest complexation of PS-CMCD and HCPT and followed by surface modification of hyaluronic acid (HA) with CD44 receptor-targeting properties. The successful inclusion was also validated through computer simulation. The obtained nanocomposites displayed the esterase-responsive release behaviors of HCPT. Moreover, the synthesized PS-CMCD/HCPT/HA NPs enhanced the intracellular drug uptake due to the tumor cell- and nuclear-mediated targeting. In addition, in vivo application exhibited that PS-CMCD/HCPT/HA NPs realized good antitumor effects. These findings suggested its potential for targeted delivery and more effective tumor therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"246 ","pages":"Article 114371"},"PeriodicalIF":5.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-07DOI: 10.1016/j.colsurfb.2024.114351
Asma Tahir , Sidra Aslam , Saba Sohail , Fakhar ud Din , Ali H. Alamri , Ahmed A. Lahiq , Shaker T. Alsharif , Abdullah Asiri
The aim of this study was to develop paroxetine (PXT) loaded nanotransferosomal gel (PXT-NTFG) for intranasal brain delivery. The process involved fabricating PXT-NTFs (paroxetine-loaded nanotransferosomes) through a thin film hydration method and optimizing them based on parameters such as particle size (PS), zeta potential (ZP), polydispersity index (PDI), and entrapment efficiency (EE). The optimized PXT-NTFs exhibited uniform morphology with a PS of 158.30 ± 2.73 nm, low PDI (0.142 ± 0.072), high ZP (21.00 ± 0.75 mV), and excellent EE (88.09 ± 3.40 %). Characterization through various techniques confirmed the incorporation of PXT into the nanotransferosomes and its conversion to amorphous state. Moreover, PXT-NTFG was formulated with suitable viscosity and mucoadhesive properties. In vitro release studies demonstrated sustained drug release from PXT-NTFG at different pH levels as compared to PXT-NTFs and NTF dispersion. Similarly, ex vivo experiments showed 4 folds enhanced drug permeation from PXT-NTFG when compared with PXT conventional gel. Stability studies indicated that the optimized PXT-NTFs remained stable for four months at 4°C and 25°C. Additionally, improved behavioral outcomes, increased neuronal survival rates, and upregulated brain-derived neurotrophic factor (BDNF) expression was observed in lipopolysaccharide (LPS) induced depressed Sprague-Dawley rats after treatment with PXT-NTFG as compared to PXT-dispersion treated and untreated LPS-control groups. Notably, the formulation led to a significant reduction in brain and plasma TNF-α levels. In conclusion, intranasal PXT-NTFG is a promising formulation with sustained drug release, improved brain targeting and enhanced antidepressant activity.
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