Ginsenoside 20(S)-Rg3 Hinders Esophageal Squamous Cell Carcinoma Cells Malignant Behaviors by miR-210-3p/B4GALT5 Axis.

IF 1.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Cell Biochemistry and Biophysics Pub Date : 2024-10-18 DOI:10.1007/s12013-024-01566-5

Min Jiang, Hong Yu

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Abstract

Ginsenoside 20(S)-Rg3 (20(S)-Rg3) belongs to a natural chemical with an anti-tumor function, but its potential function and underlying mechanism in esophageal squamous cell carcinoma (ESCC) are unknown. Several reports have manifested that microRNA (miRNA) miR-210-3p functions as a tumor repressor in tumors, but its biofunction in ESCC remains obscure. Herein, the role and interaction of 20(S)-Rg3 and miR-210-3p in ESCC cells were investigated. We performed a series of functional experiments to validate that 20(S)-Rg3 notably restrained ESCC cell proliferation and migration while promoting cell apoptosis. Besides, miR-210-3p was found to be lowly expressed in ESCC cells. Overexpressing miR-210-3p suppressed the malignant behaviors of ESCC cells. More importantly, 20(S)-Rg3 could upregulate miR-210-3p expression in ESCC cells. MiR-210-3p knockdown offset the inhibitive impacts of 20(S)-Rg3 treatment on ESCC cell growth and migration. Furthermore, through luciferase reporter assay, beta-1,4-galactosyltransferase 5 (B4GALT5) was certified to be targeted by miR-210-3p. B4GALT5 upregulation neutralized the suppressive function of 20(S)-Rg3 on ESCC progression. Overall, 20(S)-Rg3 attenuated malignant behaviors of ESCC cells by modulating miR-210-3p/B4GALT5 axis, indicating 20(S)-Rg3 has therapeutic potential for ESCC.

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人参皂苷 20(S)-Rg3 通过 miR-210-3p/B4GALT5 轴抑制食管鳞状细胞癌细胞的恶性行为

人参皂苷20(S)-Rg3（20(S)-Rg3）是一种具有抗肿瘤功能的天然化学物质，但它在食管鳞状细胞癌（ESCC）中的潜在功能和内在机制尚不清楚。一些报道表明，微RNA（miRNA）miR-210-3p在肿瘤中具有肿瘤抑制因子的功能，但其在ESCC中的生物功能仍不明确。本文研究了20(S)-Rg3和miR-210-3p在ESCC细胞中的作用和相互作用。我们进行了一系列功能实验，验证了20（S）-Rg3能显著抑制ESCC细胞的增殖和迁移，同时促进细胞凋亡。此外，我们还发现 miR-210-3p 在 ESCC 细胞中低表达。过表达 miR-210-3p 可抑制 ESCC 细胞的恶性行为。更重要的是，20(S)-Rg3 能上调 ESCC 细胞中 miR-210-3p 的表达。MiR-210-3p的敲除抵消了20（S）-Rg3对ESCC细胞生长和迁移的抑制作用。此外，通过荧光素酶报告实验，β-1,4-半乳糖基转移酶 5（B4GALT5）被证实是 miR-210-3p 的靶标。B4GALT5 的上调中和了 20(S)-Rg3 对 ESCC 进展的抑制作用。总之，20(S)-Rg3通过调节miR-210-3p/B4GALT5轴减弱了ESCC细胞的恶性行为，表明20(S)-Rg3具有治疗ESCC的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

7.5 months

期刊介绍： Cell Biochemistry and Biophysics (CBB) aims to publish papers on the nature of the biochemical and biophysical mechanisms underlying the structure, control and function of cellular systems The reports should be within the framework of modern biochemistry and chemistry, biophysics and cell physiology, physics and engineering, molecular and structural biology. The relationship between molecular structure and function under investigation is emphasized. Examples of subject areas that CBB publishes are: · biochemical and biophysical aspects of cell structure and function; · interactions of cells and their molecular/macromolecular constituents; · innovative developments in genetic and biomolecular engineering; · computer-based analysis of tissues, cells, cell networks, organelles, and molecular/macromolecular assemblies; · photometric, spectroscopic, microscopic, mechanical, and electrical methodologies/techniques in analytical cytology, cytometry and innovative instrument design For articles that focus on computational aspects, authors should be clear about which docking and molecular dynamics algorithms or software packages are being used as well as details on the system parameterization, simulations conditions etc. In addition, docking calculations (virtual screening, QSAR, etc.) should be validated either by experimental studies or one or more reliable theoretical cross-validation methods.