A New Bacterial Strain Producing Both of the Surfactin and Fengycin Lipopeptide Biosurfactant with Strong Emulsifications on Crude Oil.

IF 3.1 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Applied Biochemistry and Biotechnology Pub Date : 2024-10-15 DOI:10.1007/s12010-024-05076-1
Gui-Na Qi, Wan-Qi Qin, Guo-Jun Li, Ting-Ting Ma, Yi-Fan Liu, Lei Zhou, Jin-Feng Liu, Hong-Ze Gang, Shi-Zhong Yang, Bo-Zhong Mu
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Abstract

A new lipopeptide-producing strain Cytobacillus sp. R3-1 was isolated from the production water of the Daqing oilfield in China and identified based on 16S rRNA gene sequence analyses. The strain R3-1 is capable of simultaneously producing both of the surfactin and fengycin, the two major families of the lipopeptide biosurfactant. The chemical structures of the surfactin and fengycin were confirmed by a combination of the ESI-MS, FT-IR, and amino acid analyses, and the impact of various temperatures, pH, and NaCl concentrations on the emulsifying activity (E24) was investigated. The lipopeptide biosurfactant produced by the strain R3-1 exhibited strong emulsifying activity with E24 value over 60% on crude oil and different hydrocarbons, including the cyclohexane, hexadecane, benzene, toluene, kerosene, diesel oil, and liquid paraffin. Meanwhile, it showed excellent emulsifying activity across a broad range of conditions of the temperature up to 60 °C, NaCl tolerance up to 100 g/L, and pH values between 5 and 9, which suggests that the strain R3-1 is a valuable microbial candidate for the simultaneous production of the surfactin and fengycin lipopeptide biosurfactant with strong emulsifying properties and stability under diverse environmental conditions and is a potential application in environmental bioremediation and enhanced oil recovery.

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同时产生表面活性剂和凤仙脂肽生物表面活性剂并对原油具有强乳化作用的新菌株。
根据 16S rRNA 基因序列分析,从中国大庆油田采出水中分离出一株新的产脂肽菌株 Cytobacillus sp.菌株 R3-1 能够同时产生表面活性剂和芬吉星这两种脂肽类生物表面活性剂。通过 ESI-MS、FT-IR 和氨基酸分析,确认了表面活性剂和芬吉星的化学结构,并研究了不同温度、pH 值和 NaCl 浓度对乳化活性(E24)的影响。R3-1菌株产生的脂肽生物表面活性剂对原油和不同烃类(包括环己烷、十六烷、苯、甲苯、煤油、柴油和液体石蜡)具有很强的乳化活性,E24值超过60%。这表明菌株 R3-1 是同时生产表面活性剂和芬吉素脂肽生物表面活性剂的重要微生物候选菌株,具有很强的乳化性能和在不同环境条件下的稳定性,有望应用于环境生物修复和提高石油采收率。
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来源期刊
Applied Biochemistry and Biotechnology
Applied Biochemistry and Biotechnology 工程技术-生化与分子生物学
CiteScore
5.70
自引率
6.70%
发文量
460
审稿时长
5.3 months
期刊介绍: This journal is devoted to publishing the highest quality innovative papers in the fields of biochemistry and biotechnology. The typical focus of the journal is to report applications of novel scientific and technological breakthroughs, as well as technological subjects that are still in the proof-of-concept stage. Applied Biochemistry and Biotechnology provides a forum for case studies and practical concepts of biotechnology, utilization, including controls, statistical data analysis, problem descriptions unique to a particular application, and bioprocess economic analyses. The journal publishes reviews deemed of interest to readers, as well as book reviews, meeting and symposia notices, and news items relating to biotechnology in both the industrial and academic communities. In addition, Applied Biochemistry and Biotechnology often publishes lists of patents and publications of special interest to readers.
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