Light-adapted flicker-optoretinography based on raster-scan optical coherence tomography towards clinical translation.

IF 2.9 2区 医学 Q2 BIOCHEMICAL RESEARCH METHODS Biomedical optics express Pub Date : 2024-09-26 eCollection Date: 2024-10-01 DOI:10.1364/BOE.538481
Zhaoyu Gong, Yaping Shi, Jian Liu, Ramkumar Sabesan, Ruikang K Wang
{"title":"Light-adapted flicker-optoretinography based on raster-scan optical coherence tomography towards clinical translation.","authors":"Zhaoyu Gong, Yaping Shi, Jian Liu, Ramkumar Sabesan, Ruikang K Wang","doi":"10.1364/BOE.538481","DOIUrl":null,"url":null,"abstract":"<p><p>Optoretinography (ORG) is a promising non-invasive and objective technique for assessing retinal function by measuring its response to light stimulation. Optical coherence tomography (OCT) has emerged as a promising tool for implementing ORG due to its three-dimensional imaging capabilities, high sensitivity to nanometer-scale changes induced by light stimulation, and clinical availability. Although ORG has proven feasible in laboratory settings, research-grade OCT systems lack satisfactory usability and cost-effectiveness to be clinically viable. Standard clinical raster-scan OCT systems, with their limited imaging speed, fall short of the requirements for measuring rapid ORG responses. To bridge this gap, we introduce a flicker-ORG modality based on a raster-scan OCT system that resembles standard clinical OCT. This system overcomes speed limitations through an innovative two-stage scanning protocol coupled with a 600 kHz swept source, enabling repeated volume imaging and precise retinal activity measurements over a finite area. Additionally, the light-adapted ORG strategy eliminates the need for dark adaptation, allowing examinations under photopic conditions and thus improving patient compliance. We tested this new ORG method by measuring flicker-induced photoreceptor responses in five healthy subjects. The results demonstrated high repeatability and revealed dependencies of the ORG response on flicker frequency and retinal eccentricity. These findings, combined with the system's utility, cost-effectiveness, and ease of integration into existing technologies, underscore its substantial potential for clinical application.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11482172/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical optics express","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1364/BOE.538481","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

Optoretinography (ORG) is a promising non-invasive and objective technique for assessing retinal function by measuring its response to light stimulation. Optical coherence tomography (OCT) has emerged as a promising tool for implementing ORG due to its three-dimensional imaging capabilities, high sensitivity to nanometer-scale changes induced by light stimulation, and clinical availability. Although ORG has proven feasible in laboratory settings, research-grade OCT systems lack satisfactory usability and cost-effectiveness to be clinically viable. Standard clinical raster-scan OCT systems, with their limited imaging speed, fall short of the requirements for measuring rapid ORG responses. To bridge this gap, we introduce a flicker-ORG modality based on a raster-scan OCT system that resembles standard clinical OCT. This system overcomes speed limitations through an innovative two-stage scanning protocol coupled with a 600 kHz swept source, enabling repeated volume imaging and precise retinal activity measurements over a finite area. Additionally, the light-adapted ORG strategy eliminates the need for dark adaptation, allowing examinations under photopic conditions and thus improving patient compliance. We tested this new ORG method by measuring flicker-induced photoreceptor responses in five healthy subjects. The results demonstrated high repeatability and revealed dependencies of the ORG response on flicker frequency and retinal eccentricity. These findings, combined with the system's utility, cost-effectiveness, and ease of integration into existing technologies, underscore its substantial potential for clinical application.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
基于光栅扫描光学相干断层扫描的光适配闪烁视网膜成像技术走向临床转化。
光学视网膜造影术(ORG)是通过测量视网膜对光刺激的反应来评估视网膜功能的一种很有前途的非侵入性客观技术。光学相干断层扫描(OCT)因其三维成像能力、对光刺激引起的纳米级变化的高灵敏度和临床可用性,已成为实施视网膜视网膜造影术的理想工具。尽管 ORG 在实验室环境中已被证明是可行的,但研究级 OCT 系统缺乏令人满意的可用性和成本效益,因此在临床上并不可行。标准的临床光栅扫描 OCT 系统成像速度有限,无法满足测量快速 ORG 反应的要求。为了弥补这一差距,我们在光栅扫描 OCT 系统的基础上引入了闪烁 ORG 模式,该模式与标准临床 OCT 相似。该系统通过创新的两阶段扫描协议和 600 kHz 扫频光源克服了速度限制,实现了有限区域内的重复体积成像和精确视网膜活动测量。此外,光适应 ORG 策略无需暗适应,允许在光照条件下进行检查,从而提高了患者的依从性。我们通过测量五名健康受试者闪烁诱导的感光器反应,测试了这种新的 ORG 方法。结果表明,ORG 反应具有很高的可重复性,并且与闪烁频率和视网膜偏心率有关。这些发现,加上该系统的实用性、成本效益以及与现有技术集成的便捷性,凸显了其在临床应用方面的巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Biomedical optics express
Biomedical optics express BIOCHEMICAL RESEARCH METHODS-OPTICS
CiteScore
6.80
自引率
11.80%
发文量
633
审稿时长
1 months
期刊介绍: The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including: Tissue optics and spectroscopy Novel microscopies Optical coherence tomography Diffuse and fluorescence tomography Photoacoustic and multimodal imaging Molecular imaging and therapies Nanophotonic biosensing Optical biophysics/photobiology Microfluidic optical devices Vision research.
期刊最新文献
Correlating stroke risk with non-invasive cerebrovascular perfusion dynamics using a portable speckle contrast optical spectroscopy laser device. Improvements on speed, stability and field of view in adaptive optics OCT for anterior retinal imaging using a pyramid wavefront sensor. Light-adapted flicker-optoretinography based on raster-scan optical coherence tomography towards clinical translation. Photodynamic opening of the blood-brain barrier affects meningeal lymphatics and the brain's drainage in healthy male mice. Stand-alone segmentation of blood flow pulsatility measured with diffuse correlation spectroscopy.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1