Toxicological screening of zinc oxide nanoparticles in mongrel dogs after seven days of repeated subcutaneous injections.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES BMC Veterinary Research Pub Date : 2024-10-18 DOI:10.1186/s12917-024-04268-5
Marwa H Hassan, Ibrahim A Emam, Haitham Farghali, Marwa A Ibrahim, Neven H Hassan, Khaled Y Farroh, Eman I Hassanen
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Abstract

Zinc oxide nanoparticles (ZnO NPs) have recently been applied in various veterinary and medical fields, however, the toxicological evaluations of these NPs in dogs are lacking. Therefore, the current study is designed to assess the impact of exposure to daily subcutaneous (SC) injections of ZnO NPs at different concentrations on various organs of mongrel dogs. Nine dogs were randomly divided into three groups (n = 3 for each) as follows: group (1) served as the control group, whereas groups (2&3) received SC injections of 50 and 100 ppm ZnO NPs (8 and 16 μg/kg bwt), respectively, once/day for 7 days. Our results revealed that ZnO NPs disrupted the oxidant/antioxidant balance in the lungs, liver, and kidneys of dogs in a dose-dependent manner. ZnO NPs induced dose-dependent radiological, ultrasonographical, and histopathological alterations in various organs especially lungs, spleen, liver, and kidneys along with disturbance in both liver and kidney biomarkers levels. Most organs of both ZnO NPs receiving groups displayed strong caspase-3 protein expression. Additionally, it upregulates the transcriptase levels of TNF-α and VEGF, as well as downregulates the antiapoptotic gene IL-10 in lung, kidney, and liver tissue homogenates. It was concluded that the daily SC injections of dogs with ZnO NPs at concentrations of 50 and 100 ppm caused extensive oxidative stress damage in various organs which provoked serious pathological processes such as apoptosis and inflammation.

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经过七天反复皮下注射后,对杂种狗体内氧化锌纳米颗粒的毒理学筛选。
氧化锌纳米粒子(ZnO NPs)最近已被应用于各种兽医和医疗领域,但对这些纳米粒子在狗体内的毒理学评估还很缺乏。因此,本研究旨在评估每天皮下注射不同浓度的氧化锌纳米粒子对杂种狗各器官的影响。九只狗被随机分为以下三组(每组 n = 3):第(1)组为对照组,第(2)组和第(3)组每天分别皮下注射一次 50 ppm 和 100 ppm 的氧化锌氮氧化物(8 微克和 16 微克/千克体重),连续注射 7 天。我们的研究结果表明,氧化锌氮氧化物以剂量依赖的方式破坏了狗肺、肝脏和肾脏中的氧化剂/抗氧化剂平衡。氧化锌氮氧化物会诱导各器官(尤其是肺、脾、肝和肾)发生剂量依赖性的放射学、超声波和组织病理学改变,同时还会扰乱肝脏和肾脏的生物标志物水平。两组 ZnO NPs 受体的大多数器官都显示出较强的 caspase-3 蛋白表达。此外,它还能上调肺、肾和肝组织匀浆中 TNF-α 和血管内皮生长因子的转录酶水平,并下调抗凋亡基因 IL-10。研究得出结论,每天给狗注射浓度为 50 和 100 ppm 的氧化锌氮氧化物会对各器官造成广泛的氧化应激损伤,引发严重的病理过程,如细胞凋亡和炎症。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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