Genetic dissection of Meloidogyne incognita resistance genes based on VIGS functional analysis in Cucumis metuliferus.

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL ACS Applied Energy Materials Pub Date : 2024-10-15 DOI:10.1186/s12870-024-05681-6

Xiaoxiao Xie, Jian Ling, Junru Lu, Zhenchuan Mao, Jianlong Zhao, Shijie Zheng, Qihong Yang, Yan Li, Richard G F Visser, Yuling Bai, Bingyan Xie

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Abstract

The southern root-knot nematode, Meloidogyne incognita, is a highly serious plant parasitic nematode species that causes significant economic losses in various crops, including cucumber (Cucumis sativus L.). Currently, there are no commercial cultivars available with resistance to M. incognita in cucumber. However, the African horned melon (Cucumis metuliferus Naud.), a semi-wild relative of cucumber, has shown high resistance to M. incognita. In this study, we constructed an ultrahigh-density genetic linkage bin-map using low-coverage sequences from an F₂ population generated through the cross between C. metuliferus inbred lines CM3 and CM27. Finally, we identified a QTL (quantitative trait locus, QTL3.1) with a LOD (logarithm of the odds) score of 3.84, explaining 8.4% of the resistance variation. Subsequently, by combining the results of qPCR (quantitative PCR) and VIGS (virus-induced gene silencing), we identified two genes, EVM0025394 and EVM0006042, that are potentially involved in the resistance to M. incognita in CM3. The identification of QTLs and candidate genes in this study serve as a basis for further functional analysis and lay the groundwork for harnessing this resistance trait.

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基于 VIGS 功能分析的 Cucumis metuliferus 抗 Meloidogyne incognita 基因的遗传剖析。

南方根结线虫（Meloidogyne incognita）是一种非常严重的植物寄生线虫，对包括黄瓜（Cucumis sativus L.）在内的多种作物造成重大经济损失。目前，还没有对黄瓜中的 M. incognita 具有抗性的商业栽培品种。然而，黄瓜的半野生近缘植物非洲角瓜（Cucumis metuliferus Naud.）却表现出了对 M. incognita 的高度抗性。在本研究中，我们利用C. metuliferus近交系CM3和CM27杂交产生的F2群体中的低覆盖率序列构建了超高密度遗传连锁基因组图谱。最后，我们确定了一个 QTL（数量性状位点，QTL3.1），其 LOD（几率对数）得分为 3.84，解释了 8.4% 的抗性变异。随后，结合 qPCR（定量 PCR）和 VIGS（病毒诱导基因沉默）的结果，我们确定了 EVM0025394 和 EVM0006042 这两个基因可能参与了 CM3 对 M. incognita 的抗性。本研究对 QTL 和候选基因的鉴定为进一步的功能分析奠定了基础，并为利用这一抗性性状奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.