PirA- or PirB-binding nanobodies can protect whiteleg shrimp from the acute hepatopancreatic necrosis disease toxin.

IF 1.1 4区 农林科学 Q3 FISHERIES Diseases of aquatic organisms Pub Date : 2024-10-10 DOI:10.3354/dao03817
Sylvia Cheung, Slade A Loutet, Sarah Zaytsoff, Filip Van Petegem, Loc H Tran, Hamlet Abnousi
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Abstract

Acute hepatopancreatic necrosis disease (AHPND) is a devastating shrimp disease caused by a binary toxin, PirAB, produced by Vibrio parahaemolyticus and other closely related bacteria. To address AHPND, over 300 unique single-domain antibodies (also known as nanobodies) derived from the VHH domains of Lama glama heavy-chain-only antibodies were raised against either PirA or PirB and characterized. Nanobodies were shortlisted based on their affinities for either PirA or PirB, their relative stability in intestinal fluids, and their ability to reduce PirAB-induced death in brine shrimp Artemia salina. From these data, a subset of nanobodies was tested for their ability to reduce AHPND in whiteleg shrimp Penaeus vannamei, and nanobodies targeting either PirA or PirB provided significant disease protection to whiteleg shrimp. These results show that nanobodies can be a new option for shrimp farmers to reduce or eliminate the impact of AHPND on their operations.

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与 PirA 或 PirB 结合的纳米抗体可保护南美白对虾免受急性肝胰腺坏死病毒素的侵害。
急性肝胰腺坏死病(AHPND)是一种毁灭性虾病,由副溶血性弧菌和其他密切相关的细菌产生的二元毒素 PirAB 引起。为了应对 AHPND,研究人员针对 PirA 或 PirB 培育了 300 多种独特的单域抗体(也称为纳米抗体),这些抗体来自喇嘛釉重链抗体的 VHH 结构域,并对其进行了表征。根据纳米抗体对 PirA 或 PirB 的亲和力、在肠液中的相对稳定性以及减少 PirAB 诱导的盐水虾死亡的能力,筛选出了几种纳米抗体。根据这些数据,测试了一部分纳米抗体减少白对虾AHPND的能力,靶向PirA或PirB的纳米抗体为白对虾提供了显著的疾病保护。这些结果表明,纳米抗体可以成为对虾养殖者减少或消除AHPND对其经营影响的一种新选择。
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来源期刊
Diseases of aquatic organisms
Diseases of aquatic organisms 农林科学-兽医学
CiteScore
3.10
自引率
0.00%
发文量
53
审稿时长
8-16 weeks
期刊介绍: DAO publishes Research Articles, Reviews, and Notes, as well as Comments/Reply Comments (for details see DAO 48:161), Theme Sections and Opinion Pieces. For details consult the Guidelines for Authors. Papers may cover all forms of life - animals, plants and microorganisms - in marine, limnetic and brackish habitats. DAO''s scope includes any research focusing on diseases in aquatic organisms, specifically: -Diseases caused by coexisting organisms, e.g. viruses, bacteria, fungi, protistans, metazoans; characterization of pathogens -Diseases caused by abiotic factors (critical intensities of environmental properties, including pollution)- Diseases due to internal circumstances (innate, idiopathic, genetic)- Diseases due to proliferative disorders (neoplasms)- Disease diagnosis, treatment and prevention- Molecular aspects of diseases- Nutritional disorders- Stress and physical injuries- Epidemiology/epizootiology- Parasitology- Toxicology- Diseases of aquatic organisms affecting human health and well-being (with the focus on the aquatic organism)- Diseases as indicators of humanity''s detrimental impact on nature- Genomics, proteomics and metabolomics of disease- Immunology and disease prevention- Animal welfare- Zoonosis
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