Genomic characterization of MRSA recovered from people with cystic fibrosis during two Spanish multicentre studies (2013 and 2021).

IF 3.7 Q2 INFECTIOUS DISEASES JAC-Antimicrobial Resistance Pub Date : 2024-10-17 eCollection Date: 2024-10-01 DOI:10.1093/jacamr/dlae160
Ainhize Maruri-Aransolo, Marta Hernandez-García, Raquel Barbero, Malkoa Michelena, María Dolores Pastor-Vivero, Pedro Mondejar-Lopez, Amparo Solé, Rafael Cantón, Juan de Dios Caballero-Pérez
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引用次数: 0

Abstract

Background: Chronic bronchopulmonary infection due to MRSA in people with cystic fibrosis (pwCF) has been associated with accelerated decline in lung function, increased hospitalizations and increased mortality.

Material and methods: We studied microbiological and genomic characteristics of MRSA isolates recovered from pwCF in two Spanish multicentre studies (2013, 2021). Antimicrobial susceptibility was performed. WGS was carried out to determine population structure [MLST, spa-typing, staphylococcal cassette chromosome mec (SCCmec)], resistome and virulome. Clinical charts of MRSA-infected and MRSA-non-infected pwCF were also reviewed.

Results: MRSA infection prevalence decreased between 2013 (29/341, 8.5%) and 2021 (21/326, 6.4%) (P = 0.378). Differences in lung function were observed between infected and non-infected patients (P < 0.005). A higher prevalence of hospital-acquired (HA) clones was found compared with community-acquired (CA) clones (2013: 67% versus 33%; and 2021: 71% versus 29%). Overall, we noted clustering of isolates based on year of sampling, type of acquisition and clonal complex (CC). HA-MRSA population was dominated by CC5, with ST125-MRSA-IVc-t067 the most prevalent lineage (37%). A higher clonal diversity was detected among CA-MRSA. One Panton-Valentine leucocidin (PVL)-positive strain (ST8-MRSA-IV) and three strains of porcine origin (two ST398-MRSA-V-t011, one ST398-MRSA-V-t8567) were found. Additionally, acquired resistance genes (n = 24) were detected, including the cfr gene conferring linezolid resistance. A higher gentamicin resistance was found in 2021 (42%) compared with 2013 (7%) (P = 0.046), associated with the aac(6')-aph(2″) gene.

Conclusions: Despite a decrease in MRSA prevalence, we showed its potential impact on CF severity and progression. Moreover, we observed great genotypic and phenotypic diversity in MRSA isolates from pwCF as well as an MDR trait.

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在两项西班牙多中心研究(2013 年和 2021 年)中,从囊性纤维化患者身上发现的 MRSA 的基因组特征。
背景:囊性纤维化患者(pwCF)因 MRSA 引起的慢性支气管肺部感染与肺功能加速衰退、住院率增加和死亡率上升有关:我们研究了两项西班牙多中心研究(2013 年和 2021 年)中从囊性纤维化患者中分离出的 MRSA 的微生物学和基因组特征。进行了抗菌药物药敏试验。通过 WGS 确定了种群结构 [MLST、spa-typing、葡萄球菌盒式染色体 mec (SCCmec)]、抗性组和毒力组。此外,还查阅了感染 MRSA 和未感染 MRSA 的儿童基金会的临床病历:MRSA 感染率在 2013 年(29/341,8.5%)和 2021 年(21/326,6.4%)之间有所下降(P = 0.378)。发现感染和非感染患者的肺功能存在差异(P n = 24),包括赋予利奈唑胺耐药性的 cfr 基因。与2013年(7%)相比,2021年(42%)发现的庆大霉素耐药性更高(P = 0.046),与aac(6')-aph(2″)基因有关:结论:尽管MRSA的流行率有所下降,但我们发现它对CF的严重性和进展有潜在影响。此外,我们还观察到来自 pwCF 的 MRSA 分离物具有极大的基因型和表型多样性以及 MDR 特性。
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CiteScore
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16 weeks
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