Facile and Sustainable Electrostatic Integration of Eosin Y Dye for the Assay of the Atomoxetine Drug Through the Enhancement of the RRS Phenomenon.

Abstract

The suggested study follows specific protocols to guarantee that the atomoxetine drug analysis approach is environmentally friendly and sustainable. A number of recently created methods were used as prospective evidence for environmental sustainability and applicability, which is an essential point to emphasize. The current study introduces a new and very unique technology using ultrasensitive spectrofluorimetry to identify the atomoxetine (AXT) medication. A one-step, one-pot, direct spectrofluorimetric technique was used in this study's methodology, which was determined to be effective and environmentally sustainable for the drug's evaluation and validation. When AXT and EY reagent were mixed in an acidic setting, a highly resonant Rayleigh scattering product was promptly generated. The application of fluorimetric analysis was built on a novel theory that the augmentation in dye response subsequent to the addition of AXT was directly correlated to the resultant complex's molecular mass, as determined at a wavelength of 365 nm. The complexation progression caused a considerable rise in the molecular mass, from 292.82 (AXT⁺) to 983.68 (AXT-EY) g mol^-1. The quantum yield for the coupled product was measured. The linearity was determined to be between 30 and 1500 ng mL^-1, while the sensitivity values were found to be between 9.2 and 28.1 ng mL^-1. Analyzing AXT-EY complexes allowed us to determine the best values for all of the system's adjustable parameters. The system showed adherence to International Council for Harmonization (ICH) criteria without difficulties. The recommended procedure was then evaluated for environmental friendliness using many current environmental safety metrics. Fortunately, the current analytical technique is also recognized as a white one by the WAC standards, which integrate functional and ecological features using the Green/Red/Blue (RGB 12) design. A novel instrument called BAGI was used to assess the feasibility of the proposed approach in the field of analytical chemistry.