Fluorescent Determination of Uric Acid Based on Porphyrin and ZnCo₂O₄ Nanocomposite.

IF 2.6 4区化学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Fluorescence Pub Date : 2024-10-19 DOI:10.1007/s10895-024-03986-1

Shijo Francis, Shamna Salim, Leena Rajith

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引用次数: 0

Abstract

Advances in porphyrin chemistry have provided exciting technologies in the field of optical biosensing. Herein, we have synthesized 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin (TCPP) and porous Zn_0.1Co₂O₄ nanorods using a simple one-pot hydrothermal method. The obtained TCPP- Zn_0.1Co₂O₄ composite was then used for the development of a novel optical sensor for the determination of uric acid (UA), which is an important biomarker in human urine, serum or saliva for the clinical diagnosis of hyperuricemia and hypouricemia, etc. TCPP-Zn_0.1Co₂O₄ composite was characterized using SEM, TEM, EDAX, PXRD, FT-IR, UV-Visible, and NMR spectroscopic techniques. The fluorescence emission spectral analysis of TCPP-Zn_0.1Co₂O₄ was then investigated for potential applications in the detection of uric acid via the fluorescence quenching mechanism. The designed sensor showed a linear response towards the uric acid in the concentration range of 0.99 to 5.2 nM. The optical sensor exhibits a sensitive response to uric acid with a detection limit of 0.015 nM. The sensor was employed to quantify UA in spiked human urine samples and artificial urine with satisfactory results.

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基于卟啉和 ZnCo2O4 纳米复合材料的尿酸荧光测定。

卟啉化学的进步为光学生物传感领域提供了令人兴奋的技术。在此，我们采用简单的一锅水热法合成了 5,10,15,20-四（4-羧基苯基）卟啉（TCPP）和多孔 Zn0.1Co2O4 纳米棒。尿酸是人体尿液、血清或唾液中的重要生物标志物，可用于临床诊断高尿酸血症和低尿酸血症等。利用 SEM、TEM、EDAX、PXRD、FT-IR、UV-Visible 和 NMR 光谱技术对 TCPP-Zn0.1Co2O4 复合材料进行了表征。然后对 TCPP-Zn0.1Co2O4 的荧光发射光谱进行了分析，研究其通过荧光淬灭机制检测尿酸的潜在应用。所设计的传感器在 0.99 至 5.2 nM 的浓度范围内对尿酸呈线性响应。该光学传感器对尿酸反应灵敏，检测限为 0.015 nM。该传感器被用于定量检测添加了尿酸的人体尿液样本和人工尿液，结果令人满意。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Fluorescence 化学-分析化学

CiteScore

4.60

自引率

7.40%

发文量

203

审稿时长

5.4 months

期刊介绍： Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.