Green Protocol for the Synthesis of Luminescent Carbon Nanodots Derived from Murraya Koenigii Leaves and Urea: Enhancing Photoluminescence for Advanced Bioimaging.

IF 2.6 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Fluorescence Pub Date : 2024-10-16 DOI:10.1007/s10895-024-03953-w
Urvi M Lad, Dhruti J Dave, Bhumi N Desai, Devesh H Suthar, Chetan K Modi
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Abstract

We have synthesized Murraya Koenigii leaves powder-derived carbon nanodots (CNDs) by hydrothermal method. A tribute to our commitment to environmental sustainability is the unique composition of our CNDs, which are made entirely of natural carbon sources and a green solvent, water. Our further efforts to improve performance led us to start making nitrogen-doped CNDs. By using urea as a non-toxic source of nitrogen, we observed a substantial increase in fluorescence intensity, extending the usefulness and potential of these nanomaterials. We investigated the optical properties using UV-Vis and fluorescence spectroscopy. The other parameters, like structural and size-shape morphology, were analyzed using FTIR, XRD, and HR-TEM, respectively. The fluorescence spectroscopy demonstrated their capability to exhibit wavelength-dependent photoluminescence (PL), highlighting the potential of these CNDs for cell bioimaging applications. The fluorescence properties affirm their suitability for biomedical applications, as they do not involve any inherent risk to cells.

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从鹅掌楸叶和尿素中合成发光碳纳米点的绿色方法:增强先进生物成像的光致发光。
我们通过水热法合成了源自墨累利叶粉末的碳纳米点(CNDs)。我们的 CND 具有独特的成分,完全由天然碳源和绿色溶剂(水)制成,这体现了我们对环境可持续发展的承诺。为了进一步提高性能,我们开始生产掺氮 CND。通过使用尿素作为无毒的氮源,我们观察到荧光强度大幅提高,从而拓展了这些纳米材料的用途和潜力。我们使用紫外可见光谱和荧光光谱对其光学特性进行了研究。傅立叶变换红外光谱、X 射线衍射和 HR-TEM 分别分析了结构和尺寸-形状形态等其他参数。荧光光谱分析表明,它们具有随波长变化的光致发光(PL)能力,凸显了这些 CNDs 在细胞生物成像应用方面的潜力。荧光特性证实了它们适合生物医学应用,因为它们不会对细胞造成任何固有风险。
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来源期刊
Journal of Fluorescence
Journal of Fluorescence 化学-分析化学
CiteScore
4.60
自引率
7.40%
发文量
203
审稿时长
5.4 months
期刊介绍: Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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