Renal denervation ameliorates atrial remodeling in type 2 diabetic rats by regulating mitochondrial dynamics.

IF 3.7 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of physiology and biochemistry Pub Date : 2024-10-22 DOI:10.1007/s13105-024-01054-7
Jun-Yu Huo, Can Hou, Xiao-Long Li, Ling Yang, Wan-Ying Jiang
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Abstract

There is no effective treatment for diabetes-related atrial remodeling currently. This study aimed to investigate the effects of renal denervation (RDN) on diabetes-related atrial remodeling and explore the related mechanisms. A type 2 diabetes mellitus model was established by high-fat diet feeding and low-dose streptozotocin injection in Sprague‒Dawley rats. After successful modeling, the diabetic rats were randomly assigned to two groups according to whether they were subjected to RDN or sham RDN surgery. At the end of the experiment, cardiac function and structure were evaluated by echocardiography and histology, respectively. Mitochondrial morphology, function and mitochondrial dynamics were assessed by multiple methods. Mdivi1 was used to verify the mechanism by which RDN improves atrial remodeling. In the 10th week, diabetic rats exhibited obvious atrial remodeling, including atrial enlargement and diastolic dysfunction. Pathological staining showed that diabetic rats had cardiomyocyte hypertrophy and interstitial fibrosis in atrial tissues. In terms of mitochondrial morphology and function, diabetic rats exhibited fragmented mitochondria, reduced adenosine triphosphate production and decreased mitochondrial membrane potential levels. Abnormal mitochondrial dynamics in diabetic rats were characterized by the inhibition of mitochondrial fusion, excessive mitochondrial fission, and the suppression of mitophagy. However, RDN effectively ameliorated diabetes-induced pathological atrial remodeling. In addition, RDN significantly improved mitochondrial morphological and functional abnormalities and corrected the disorders of mitochondrial dynamics. Furthermore, the protective effects of RDN against atrial remodeling were related to the regulation of mitochondrial dynamics. RDN prevented diabetes-induced atrial remodeling. These protective effects might be related to improvements in mitochondrial dynamics.

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通过调节线粒体动力学,肾脏去神经化可改善 2 型糖尿病大鼠的心房重塑。
目前还没有治疗糖尿病相关心房重塑的有效方法。本研究旨在探讨肾脏去神经(RDN)对糖尿病相关心房重塑的影响及相关机制。通过高脂饮食喂养和小剂量链脲佐菌素注射,建立了 Sprague-Dawley 大鼠 2 型糖尿病模型。建模成功后,根据糖尿病大鼠是否接受 RDN 或假 RDN 手术将其随机分为两组。实验结束后,分别用超声心动图和组织学方法评估心脏功能和结构。线粒体形态、功能和线粒体动力学通过多种方法进行评估。Mdivi1 被用来验证 RDN 改善心房重塑的机制。在第 10 周,糖尿病大鼠表现出明显的心房重塑,包括心房扩大和舒张功能障碍。病理染色显示,糖尿病大鼠心肌细胞肥大,心房组织间质纤维化。在线粒体形态和功能方面,糖尿病大鼠表现出线粒体破碎、三磷酸腺苷生成减少和线粒体膜电位水平降低。糖尿病大鼠线粒体动力学异常的特点是线粒体融合受抑制、线粒体裂变过多和有丝分裂受抑制。然而,RDN 能有效改善糖尿病引起的心房病理性重塑。此外,RDN 还能明显改善线粒体形态和功能异常,纠正线粒体动力学紊乱。此外,RDN 对心房重塑的保护作用与线粒体动力学的调节有关。RDN 可预防糖尿病诱发的心房重塑。这些保护作用可能与线粒体动力学的改善有关。
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来源期刊
Journal of physiology and biochemistry
Journal of physiology and biochemistry 生物-生化与分子生物学
CiteScore
6.60
自引率
0.00%
发文量
86
审稿时长
6-12 weeks
期刊介绍: The Journal of Physiology and Biochemistry publishes original research articles and reviews describing relevant new observations on molecular, biochemical and cellular mechanisms involved in human physiology. All areas of the physiology are covered. Special emphasis is placed on the integration of those levels in the whole-organism. The Journal of Physiology and Biochemistry also welcomes articles on molecular nutrition and metabolism studies, and works related to the genomic or proteomic bases of the physiological functions. Descriptive manuscripts about physiological/biochemical processes or clinical manuscripts will not be considered. The journal will not accept manuscripts testing effects of animal or plant extracts.
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