High-throughput, low-cost FLASH: irradiation of Drosophila melanogaster with low-energy X-rays using time structures spanning conventional and ultrahigh dose rates.

IF 1.9 4区 医学 Q2 BIOLOGY Journal of Radiation Research Pub Date : 2024-10-18 DOI:10.1093/jrr/rrae079
Alexander Hart, Jan P Dudzic, Jameson W Clarke, Jonathan Eby, Steve J Perlman, Magdalena Bazalova-Carter
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Abstract

FLASH radiotherapy is an emerging technique in radiation oncology that may improve clinical outcomes by reducing normal tissue toxicities. The physical radiation characteristics needed to induce the radiobiological benefits of FLASH are still an active area of investigation. To determine the dose rate, range of doses and delivery time structure necessary to trigger the FLASH effect, Drosophila melanogaster were exposed to ultrahigh dose rate (UHDR) or conventional radiotherapy dose rate (CONV) 120-kVp X-rays. A conventional X-ray tube outfitted with a shutter system was used to deliver 17- to 44-Gy doses to third-instar D. melanogaster larvae at both UHDR (210 Gy/s) and CONV (0.2-0.4 Gy/s) dose rates. The larvae were then tracked through development to adulthood and scored for eclosion and lifespan. Larvae exposed to UHDR eclosed at higher rates and had longer median survival as adults compared to those treated with CONV at the same doses. Eclosion rates at 24 Gy were 68% higher for the UHDR group (P < 0.05). Median survival from 22 Gy was >22 days for UHDR and 17 days for CONV (P < 0.01). Two normal tissue-sparing effects were observed for D. melanogaster irradiated with UHDR 120-kVp X-rays. The effects appeared only at intermediate doses and may be useful in establishing the dose range over which the benefits of FLASH can be obtained. This work also demonstrates the usefulness of a high-throughput fruit fly model and a low-cost X-ray tube system for radiobiological FLASH research.

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高通量、低成本 FLASH:利用跨越常规和超高剂量率的时间结构,用低能量 X 射线辐照黑腹果蝇。
FLASH 放射治疗是放射肿瘤学中的一种新兴技术,可通过减少正常组织毒性来改善临床疗效。诱导 FLASH 放射生物学效益所需的物理辐射特性仍是一个活跃的研究领域。为了确定引发FLASH效应所需的剂量率、剂量范围和传输时间结构,我们将黑腹果蝇暴露在超高剂量率(UHDR)或常规放射治疗剂量率(CONV)120-kVp的X射线下。在超高剂量率(210 Gy/s)和常规放射治疗剂量率(0.2-0.4 Gy/s)下,使用装有快门系统的常规 X 射线管向黑腹果蝇三龄幼虫照射 17-Gy 至 44-Gy 的剂量。然后跟踪幼虫从发育到成年的整个过程,并对其羽化和寿命进行评分。与相同剂量的CONV相比,暴露于UHDR的幼虫羽化率更高,成虫存活中位数更长。暴露于 UHDR 的幼虫在 24 Gy 时的羽化率比暴露于 CONV 的幼虫高 68%(UHDR 为 22 天,CONV 为 17 天(P
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来源期刊
CiteScore
3.60
自引率
5.00%
发文量
86
审稿时长
4-8 weeks
期刊介绍: The Journal of Radiation Research (JRR) is an official journal of The Japanese Radiation Research Society (JRRS), and the Japanese Society for Radiation Oncology (JASTRO). Since its launch in 1960 as the official journal of the JRRS, the journal has published scientific articles in radiation science in biology, chemistry, physics, epidemiology, and environmental sciences. JRR broadened its scope to include oncology in 2009, when JASTRO partnered with the JRRS to publish the journal. Articles considered fall into two broad categories: Oncology & Medicine - including all aspects of research with patients that impacts on the treatment of cancer using radiation. Papers which cover related radiation therapies, radiation dosimetry, and those describing the basis for treatment methods including techniques, are also welcomed. Clinical case reports are not acceptable. Radiation Research - basic science studies of radiation effects on livings in the area of physics, chemistry, biology, epidemiology and environmental sciences. Please be advised that JRR does not accept any papers of pure physics or chemistry. The journal is bimonthly, and is edited and published by the JRR Editorial Committee.
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