Rapid On-Site Histology of Lung and Pleural Biopsies Using Higher Harmonic Generation Microscopy and Artificial Intelligence Analysis

IF 7.1 1区 医学 Q1 PATHOLOGY Modern Pathology Pub Date : 2024-10-16 DOI:10.1016/j.modpat.2024.100633
Laura M.G. van Huizen , Max Blokker , Johannes M.A. Daniels , Teodora Radonic , Jan H. von der Thüsen , Mitko Veta , Jouke T. Annema , Marie Louise Groot
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Abstract

Lung cancer is one of the most prevalent and lethal cancers. To improve health outcomes while reducing health care burden, it becomes crucial to move toward early detection and cost-effective workflows. Currently, there is no method for the on-site rapid histologic feedback on biopsies taken in diagnostic, endoscopic, or surgical procedures. Higher harmonic generation (HHG) microscopy is a laser-based technique that provides images of unprocessed tissue. In this study, we report the feasibility of an HHG portable microscope in the clinical workflow in terms of acquisition time, image quality, and diagnostic accuracy in suspected pulmonary and pleural malignancy. One hundred nine biopsies of 47 patients were imaged and a biopsy overview image was provided within a median acquisition time of 6 minutes after excision. The assessment by pathologists and an artificial intelligence algorithm showed that image quality was sufficient for a malignancy or nonmalignancy diagnosis in 97% of the biopsies, and 87% of the HHG images were correctly scored by the pathologists. HHG is therefore an excellent candidate to provide a rapid pathology outcome on biopsy samples, enabling immediate diagnosis and (local) treatment.
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利用高次谐波显微镜和人工智能分析对肺和胸膜活检组织进行快速现场组织学分析。
肺癌是发病率和致死率最高的癌症之一。为了改善健康状况,同时减轻医疗负担,实现早期检测和具有成本效益的工作流程至关重要。目前,还没有一种方法能对内窥镜诊断或外科手术中的活组织切片进行现场快速组织学反馈。高次谐波发生(HHG)显微镜是一种基于激光的技术,可提供未经处理的组织图像。在此,我们报告了便携式高次谐波显微镜在临床工作流程中的可行性,包括采集时间、图像质量以及对疑似肺部和胸膜恶性肿瘤的诊断准确性。我们对 47 名患者的 109 例活检组织进行了成像,并在切除后 6 分钟内提供了活检组织概览图像。病理学家和人工智能(AI)算法的评估结果显示,97%的活检图像质量足以做出恶性或非恶性诊断,87%的 HHG 图像得到了病理学家的正确评分。因此,HHG 是为活检样本提供快速病理结果的绝佳候选方案,可实现即时诊断和(局部)治疗。
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来源期刊
Modern Pathology
Modern Pathology 医学-病理学
CiteScore
14.30
自引率
2.70%
发文量
174
审稿时长
18 days
期刊介绍: Modern Pathology, an international journal under the ownership of The United States & Canadian Academy of Pathology (USCAP), serves as an authoritative platform for publishing top-tier clinical and translational research studies in pathology. Original manuscripts are the primary focus of Modern Pathology, complemented by impactful editorials, reviews, and practice guidelines covering all facets of precision diagnostics in human pathology. The journal's scope includes advancements in molecular diagnostics and genomic classifications of diseases, breakthroughs in immune-oncology, computational science, applied bioinformatics, and digital pathology.
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